Sago pith residue as alternative cheap substrate for fermentable sugars production
Sago pith residue is one of the most abundant lignocellulosic biomass which can serve as an alternative cheap substrate for fermentable sugars production. This residue is the fibrous waste left behind after the starch extraction process and contains significant amounts of starch (58%), cellulose (23...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer-Verlag
2012
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/22427/1/Sago%20pith%20residue%20as%20alternative%20cheap%20substrate%20for%20fermentable%20sugars%20production.pdf |
| _version_ | 1825946869086289920 |
|---|---|
| author | Linggang, Siren Phang, Lai Yee Wasoh@Mohamad Isa, Helmi Abd. Aziz, Suraini |
| author_facet | Linggang, Siren Phang, Lai Yee Wasoh@Mohamad Isa, Helmi Abd. Aziz, Suraini |
| author_sort | Linggang, Siren |
| collection | UPM |
| description | Sago pith residue is one of the most abundant lignocellulosic biomass which can serve as an alternative cheap substrate for fermentable sugars production. This residue is the fibrous waste left behind after the starch extraction process and contains significant amounts of starch (58%), cellulose (23%), hemicellulose (9.2%) and lignin (3.9%). The conversion of sago pith residue into fermentable sugars is commonly performed using cellulolytic enzymes or known as cellulases. In this study, crude cellulases were produced by two local isolates, Trichoderma asperellum UPM1 and Aspergillus fumigatus, UPM2 using sago pith residue as substrate. A. fumigatus UPM2 gave the highest FPase, CMCase and β-glucosidase activities of 0.39, 23.99 and 0.78 U/ml, respectively, on day 5. The highest activity of FPase, CMCase and β-glucosidase by T. asperellum UPM1 was 0.27, 12.03 and 0.42 U/ml, respectively, on day 7. The crude enzyme obtained from A. fumigatus UPM2 using β-glucosidase as the rate-limiting enzyme (3.9, 11.7 and 23.4 IU) was used for the saccharification process to convert 5% (w/v) sago pith residue into reducing sugars. Hydrolysis of sago pith residue using crude enzyme containing β-glucosidase with 23.4 IU, produced by A. fumigatus UPM2 gave higher reducing sugars production of 20.77 g/l with overall hydrolysis percentage of 73%. |
| first_indexed | 2024-03-06T07:53:54Z |
| format | Article |
| id | upm.eprints-22427 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:53:54Z |
| publishDate | 2012 |
| publisher | Springer-Verlag |
| record_format | dspace |
| spelling | upm.eprints-224272015-10-02T00:12:41Z http://psasir.upm.edu.my/id/eprint/22427/ Sago pith residue as alternative cheap substrate for fermentable sugars production Linggang, Siren Phang, Lai Yee Wasoh@Mohamad Isa, Helmi Abd. Aziz, Suraini Sago pith residue is one of the most abundant lignocellulosic biomass which can serve as an alternative cheap substrate for fermentable sugars production. This residue is the fibrous waste left behind after the starch extraction process and contains significant amounts of starch (58%), cellulose (23%), hemicellulose (9.2%) and lignin (3.9%). The conversion of sago pith residue into fermentable sugars is commonly performed using cellulolytic enzymes or known as cellulases. In this study, crude cellulases were produced by two local isolates, Trichoderma asperellum UPM1 and Aspergillus fumigatus, UPM2 using sago pith residue as substrate. A. fumigatus UPM2 gave the highest FPase, CMCase and β-glucosidase activities of 0.39, 23.99 and 0.78 U/ml, respectively, on day 5. The highest activity of FPase, CMCase and β-glucosidase by T. asperellum UPM1 was 0.27, 12.03 and 0.42 U/ml, respectively, on day 7. The crude enzyme obtained from A. fumigatus UPM2 using β-glucosidase as the rate-limiting enzyme (3.9, 11.7 and 23.4 IU) was used for the saccharification process to convert 5% (w/v) sago pith residue into reducing sugars. Hydrolysis of sago pith residue using crude enzyme containing β-glucosidase with 23.4 IU, produced by A. fumigatus UPM2 gave higher reducing sugars production of 20.77 g/l with overall hydrolysis percentage of 73%. Springer-Verlag 2012-05 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22427/1/Sago%20pith%20residue%20as%20alternative%20cheap%20substrate%20for%20fermentable%20sugars%20production.pdf Linggang, Siren and Phang, Lai Yee and Wasoh@Mohamad Isa, Helmi and Abd. Aziz, Suraini (2012) Sago pith residue as alternative cheap substrate for fermentable sugars production. Applied Biochemistry and Biotechnology, 167 (1). pp. 122-131. ISSN 0273-2289; ESSN: 1559-0291 http://link.springer.com/article/10.1007%2Fs12010-012-9592-0 10.1007/s12010-012-9592-0 |
| spellingShingle | Linggang, Siren Phang, Lai Yee Wasoh@Mohamad Isa, Helmi Abd. Aziz, Suraini Sago pith residue as alternative cheap substrate for fermentable sugars production |
| title | Sago pith residue as alternative cheap substrate for fermentable sugars production |
| title_full | Sago pith residue as alternative cheap substrate for fermentable sugars production |
| title_fullStr | Sago pith residue as alternative cheap substrate for fermentable sugars production |
| title_full_unstemmed | Sago pith residue as alternative cheap substrate for fermentable sugars production |
| title_short | Sago pith residue as alternative cheap substrate for fermentable sugars production |
| title_sort | sago pith residue as alternative cheap substrate for fermentable sugars production |
| url | http://psasir.upm.edu.my/id/eprint/22427/1/Sago%20pith%20residue%20as%20alternative%20cheap%20substrate%20for%20fermentable%20sugars%20production.pdf |
| work_keys_str_mv | AT linggangsiren sagopithresidueasalternativecheapsubstrateforfermentablesugarsproduction AT phanglaiyee sagopithresidueasalternativecheapsubstrateforfermentablesugarsproduction AT wasohmohamadisahelmi sagopithresidueasalternativecheapsubstrateforfermentablesugarsproduction AT abdazizsuraini sagopithresidueasalternativecheapsubstrateforfermentablesugarsproduction |