Utilization of oil palm decanter cake for cellulase and polyoses production
The abundance of oil palm decanter cake (OPDC) is a problem in oil palm mills. However, this lignocellulosic biomass can be utilized for cellulase and polyoses production. The effectiveness of chemical and physical pretreatment in reducing the lignin content was studied by saccharification using a C...
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| Format: | Article |
| Language: | English |
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The Korean Society for Biotechnology and Bioengineering
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22428/1/Utilization%20of%20oil%20palm%20decanter%20cake%20for%20cellulase%20and%20polyoses%20production.pdf |
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| author | Abdul Razak, Mohamad Nafis Ibrahim, Mohamad Faizal Phang, Lai Yee Hassan, Mohd Ali Abd. Aziz, Suraini |
| author_facet | Abdul Razak, Mohamad Nafis Ibrahim, Mohamad Faizal Phang, Lai Yee Hassan, Mohd Ali Abd. Aziz, Suraini |
| author_sort | Abdul Razak, Mohamad Nafis |
| collection | UPM |
| description | The abundance of oil palm decanter cake (OPDC) is a problem in oil palm mills. However, this lignocellulosic biomass can be utilized for cellulase and polyoses production. The effectiveness of chemical and physical pretreatment in reducing the lignin content was studied by saccharification using a Celluclast 1.5 L and scanning electron microscope. Physicochemical pretreatment of OPDC with 1% (w/v) NaOH and autoclaving at 121°C for 20 min increased potential polyoses produced to 52.5% and removed 28.7% of the lignin content. The optimized conditions for cellulase production by a locally isolated fungus were a time of 120 h, a substrate of untreated OPDC, a spore concentration of 1 × 107 spore/mL, a temperature of 30°C, and a pH between 7.0 and 7.5. Trichoderma asperellum UPM1 produced carboxymethylcellulase (CMCase), β-glucosidase and filter paper activity (FPase) in the following concentrations: 17.35, 0.53, and 0.28 U/mL, respectively. Aspergillus fumigatus UPM2 produced the CMCase, β-glucosidase and FPase in the following amounts: 10.93, 0.76, and 0.24 U/mL. The cellulases from T. asperellum UPM1 produced 2.33 g/L of polyoses and the cellulases from A. fumigatus UPM2 produced 4.37 g/L of polyoses. |
| first_indexed | 2024-03-06T07:53:54Z |
| format | Article |
| id | upm.eprints-22428 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:53:54Z |
| publishDate | 2012 |
| publisher | The Korean Society for Biotechnology and Bioengineering |
| record_format | dspace |
| spelling | upm.eprints-224282016-06-07T08:36:49Z http://psasir.upm.edu.my/id/eprint/22428/ Utilization of oil palm decanter cake for cellulase and polyoses production Abdul Razak, Mohamad Nafis Ibrahim, Mohamad Faizal Phang, Lai Yee Hassan, Mohd Ali Abd. Aziz, Suraini The abundance of oil palm decanter cake (OPDC) is a problem in oil palm mills. However, this lignocellulosic biomass can be utilized for cellulase and polyoses production. The effectiveness of chemical and physical pretreatment in reducing the lignin content was studied by saccharification using a Celluclast 1.5 L and scanning electron microscope. Physicochemical pretreatment of OPDC with 1% (w/v) NaOH and autoclaving at 121°C for 20 min increased potential polyoses produced to 52.5% and removed 28.7% of the lignin content. The optimized conditions for cellulase production by a locally isolated fungus were a time of 120 h, a substrate of untreated OPDC, a spore concentration of 1 × 107 spore/mL, a temperature of 30°C, and a pH between 7.0 and 7.5. Trichoderma asperellum UPM1 produced carboxymethylcellulase (CMCase), β-glucosidase and filter paper activity (FPase) in the following concentrations: 17.35, 0.53, and 0.28 U/mL, respectively. Aspergillus fumigatus UPM2 produced the CMCase, β-glucosidase and FPase in the following amounts: 10.93, 0.76, and 0.24 U/mL. The cellulases from T. asperellum UPM1 produced 2.33 g/L of polyoses and the cellulases from A. fumigatus UPM2 produced 4.37 g/L of polyoses. The Korean Society for Biotechnology and Bioengineering 2012 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22428/1/Utilization%20of%20oil%20palm%20decanter%20cake%20for%20cellulase%20and%20polyoses%20production.pdf Abdul Razak, Mohamad Nafis and Ibrahim, Mohamad Faizal and Phang, Lai Yee and Hassan, Mohd Ali and Abd. Aziz, Suraini (2012) Utilization of oil palm decanter cake for cellulase and polyoses production. Biotechnology and Bioprocess Engineering, 17 (3). pp. 547-555. ISSN 1226-8372; ESSN: 1976-3816 http://link.springer.com/article/10.1007%2Fs12257-011-0590-9 10.1007/s12257-011-0590-9 |
| spellingShingle | Abdul Razak, Mohamad Nafis Ibrahim, Mohamad Faizal Phang, Lai Yee Hassan, Mohd Ali Abd. Aziz, Suraini Utilization of oil palm decanter cake for cellulase and polyoses production |
| title | Utilization of oil palm decanter cake for cellulase and polyoses production |
| title_full | Utilization of oil palm decanter cake for cellulase and polyoses production |
| title_fullStr | Utilization of oil palm decanter cake for cellulase and polyoses production |
| title_full_unstemmed | Utilization of oil palm decanter cake for cellulase and polyoses production |
| title_short | Utilization of oil palm decanter cake for cellulase and polyoses production |
| title_sort | utilization of oil palm decanter cake for cellulase and polyoses production |
| url | http://psasir.upm.edu.my/id/eprint/22428/1/Utilization%20of%20oil%20palm%20decanter%20cake%20for%20cellulase%20and%20polyoses%20production.pdf |
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