Bioactive compounds and antioxidant activity of different extracts from Vitex negundo leaf
The study was conducted to assess the antioxidant activity of methanol and hexane extract and essential oil from Vitex negundo leaf using different in vitro antioxidant assays. Antioxidant property was tested using 2,2-diphenyl-1-picrylhydrozyl (DPPH) free radical scavenging capacity, ferric ion red...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Academic Journals
2011
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/22434/1/Bioactive%20compounds%20and%20antioxidant%20activity%20of%20different%20extracts%20from%20Vitex%20negundo%20leaf.pdf |
| _version_ | 1796970160032055296 |
|---|---|
| author | Zargar, Mohsen Abdul Hamid, Azizah Mustafa, Roheeyati Ashikin Abu Bakar, Fatimah Jahanshiri, Fatemeh Pak Dek, Mohd Sabri |
| author_facet | Zargar, Mohsen Abdul Hamid, Azizah Mustafa, Roheeyati Ashikin Abu Bakar, Fatimah Jahanshiri, Fatemeh Pak Dek, Mohd Sabri |
| author_sort | Zargar, Mohsen |
| collection | UPM |
| description | The study was conducted to assess the antioxidant activity of methanol and hexane extract and essential oil from Vitex negundo leaf using different in vitro antioxidant assays. Antioxidant property was tested using 2,2-diphenyl-1-picrylhydrozyl (DPPH) free radical scavenging capacity, ferric ion reducing antioxidant power (FRAP) and β-carotene-linoleic acid assays. Total phenolic contents (TPC) were measured using Folin-Ciocalteu method. Flavonoids, tocopherols, β-carotene and lycopene were analyzed using high performance liquid chromatography (HPLC). Results of the present study showed that methanol extract of V. negundo leaf exhibited significantly (p < 0.05) higher antioxidant activity in terms of measurements of DPPH free radical (IC50), FRAP and β-carotene-linoleic assays than those of hexane extract and essential oil. Methanol extract of V. negundo leaf also contained high amounts of bioactive compounds including total phenolic compounds (363 mg GAE/g), epicatechin (16.98 mg/g), quercetin (13.45 mg/g), catechin (8.95 mg/g) and myricetin (3.32 mg/g) while the concentrations of tocopherol, β-carotene and lycopene were found to be lower. |
| first_indexed | 2024-03-06T07:53:56Z |
| format | Article |
| id | upm.eprints-22434 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:53:56Z |
| publishDate | 2011 |
| publisher | Academic Journals |
| record_format | dspace |
| spelling | upm.eprints-224342016-06-08T01:00:58Z http://psasir.upm.edu.my/id/eprint/22434/ Bioactive compounds and antioxidant activity of different extracts from Vitex negundo leaf Zargar, Mohsen Abdul Hamid, Azizah Mustafa, Roheeyati Ashikin Abu Bakar, Fatimah Jahanshiri, Fatemeh Pak Dek, Mohd Sabri The study was conducted to assess the antioxidant activity of methanol and hexane extract and essential oil from Vitex negundo leaf using different in vitro antioxidant assays. Antioxidant property was tested using 2,2-diphenyl-1-picrylhydrozyl (DPPH) free radical scavenging capacity, ferric ion reducing antioxidant power (FRAP) and β-carotene-linoleic acid assays. Total phenolic contents (TPC) were measured using Folin-Ciocalteu method. Flavonoids, tocopherols, β-carotene and lycopene were analyzed using high performance liquid chromatography (HPLC). Results of the present study showed that methanol extract of V. negundo leaf exhibited significantly (p < 0.05) higher antioxidant activity in terms of measurements of DPPH free radical (IC50), FRAP and β-carotene-linoleic assays than those of hexane extract and essential oil. Methanol extract of V. negundo leaf also contained high amounts of bioactive compounds including total phenolic compounds (363 mg GAE/g), epicatechin (16.98 mg/g), quercetin (13.45 mg/g), catechin (8.95 mg/g) and myricetin (3.32 mg/g) while the concentrations of tocopherol, β-carotene and lycopene were found to be lower. Academic Journals 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22434/1/Bioactive%20compounds%20and%20antioxidant%20activity%20of%20different%20extracts%20from%20Vitex%20negundo%20leaf.pdf Zargar, Mohsen and Abdul Hamid, Azizah and Mustafa, Roheeyati Ashikin and Abu Bakar, Fatimah and Jahanshiri, Fatemeh and Pak Dek, Mohd Sabri (2011) Bioactive compounds and antioxidant activity of different extracts from Vitex negundo leaf. Journal of Medicinal Plants Research, 5 (12). art. no. 6A593BF23500. pp. 2525-2532. ISSN 1996-0875 http://www.academicjournals.org/journal/JMPR/article-abstract/6A593BF23500 |
| spellingShingle | Zargar, Mohsen Abdul Hamid, Azizah Mustafa, Roheeyati Ashikin Abu Bakar, Fatimah Jahanshiri, Fatemeh Pak Dek, Mohd Sabri Bioactive compounds and antioxidant activity of different extracts from Vitex negundo leaf |
| title | Bioactive compounds and antioxidant activity of different extracts from Vitex negundo leaf |
| title_full | Bioactive compounds and antioxidant activity of different extracts from Vitex negundo leaf |
| title_fullStr | Bioactive compounds and antioxidant activity of different extracts from Vitex negundo leaf |
| title_full_unstemmed | Bioactive compounds and antioxidant activity of different extracts from Vitex negundo leaf |
| title_short | Bioactive compounds and antioxidant activity of different extracts from Vitex negundo leaf |
| title_sort | bioactive compounds and antioxidant activity of different extracts from vitex negundo leaf |
| url | http://psasir.upm.edu.my/id/eprint/22434/1/Bioactive%20compounds%20and%20antioxidant%20activity%20of%20different%20extracts%20from%20Vitex%20negundo%20leaf.pdf |
| work_keys_str_mv | AT zargarmohsen bioactivecompoundsandantioxidantactivityofdifferentextractsfromvitexnegundoleaf AT abdulhamidazizah bioactivecompoundsandantioxidantactivityofdifferentextractsfromvitexnegundoleaf AT mustafaroheeyatiashikin bioactivecompoundsandantioxidantactivityofdifferentextractsfromvitexnegundoleaf AT abubakarfatimah bioactivecompoundsandantioxidantactivityofdifferentextractsfromvitexnegundoleaf AT jahanshirifatemeh bioactivecompoundsandantioxidantactivityofdifferentextractsfromvitexnegundoleaf AT pakdekmohdsabri bioactivecompoundsandantioxidantactivityofdifferentextractsfromvitexnegundoleaf |