Optimum conditions for the production of lipase by alginate-immobilized bacteria
A lipolytic Pseudomonas sp. has been successfully immobilized in strontium alginate gel bead for use in the production of lipase. This paper reports on the various conditions required for its optimum production. A 3% alginate gel concentration with a 20% (w/v) cell loading gives the highest producti...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
1992
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22565/1/Optimum%20conditions%20for%20the%20production%20of%20lipase%20by%20alginate-immobilized%20bacteria.pdf http://psasir.upm.edu.my/id/eprint/22565/7/38_429.pdf |
| _version_ | 1811137391297036288 |
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| author | Zakaria, Zainoha Abdul Razak, Che Nyonya Ampon, Kamaruzaman Basri, Mahiran Wan Yunus, Wan Md. Zin Shirai, Yoshihito Salleh, Abu Bakar Hashimoto, Kenji |
| author_facet | Zakaria, Zainoha Abdul Razak, Che Nyonya Ampon, Kamaruzaman Basri, Mahiran Wan Yunus, Wan Md. Zin Shirai, Yoshihito Salleh, Abu Bakar Hashimoto, Kenji |
| author_sort | Zakaria, Zainoha |
| collection | UPM |
| description | A lipolytic Pseudomonas sp. has been successfully immobilized in strontium alginate gel bead for use in the production of lipase. This paper reports on the various conditions required for its optimum production. A 3% alginate gel concentration with a 20% (w/v) cell loading gives the highest production of lipase. The production of lipase can be enhanced by aeration and shaking but mass transfer effect may be dependent on the ratio of cell mass and bead size. Prolonged recycling with aeration accelerates the rupturing of the beads compared to non-aerated system. A maximum production of lipase is given by 1.5g of immobilized bacteria in 50ml of culture broth. The immobilized bacteria can withstand recycling up to 24 days, (72h cycle), after which time the beads ruptured. The production, however, remains at 70%. |
| first_indexed | 2024-03-06T07:54:19Z |
| format | Article |
| id | upm.eprints-22565 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-09-25T03:33:33Z |
| publishDate | 1992 |
| publisher | Applied Microbiology, Molecular and Cellular Biosciences Research Foundation |
| record_format | dspace |
| spelling | upm.eprints-225652024-08-07T23:54:25Z http://psasir.upm.edu.my/id/eprint/22565/ Optimum conditions for the production of lipase by alginate-immobilized bacteria Zakaria, Zainoha Abdul Razak, Che Nyonya Ampon, Kamaruzaman Basri, Mahiran Wan Yunus, Wan Md. Zin Shirai, Yoshihito Salleh, Abu Bakar Hashimoto, Kenji A lipolytic Pseudomonas sp. has been successfully immobilized in strontium alginate gel bead for use in the production of lipase. This paper reports on the various conditions required for its optimum production. A 3% alginate gel concentration with a 20% (w/v) cell loading gives the highest production of lipase. The production of lipase can be enhanced by aeration and shaking but mass transfer effect may be dependent on the ratio of cell mass and bead size. Prolonged recycling with aeration accelerates the rupturing of the beads compared to non-aerated system. A maximum production of lipase is given by 1.5g of immobilized bacteria in 50ml of culture broth. The immobilized bacteria can withstand recycling up to 24 days, (72h cycle), after which time the beads ruptured. The production, however, remains at 70%. Applied Microbiology, Molecular and Cellular Biosciences Research Foundation 1992 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/22565/1/Optimum%20conditions%20for%20the%20production%20of%20lipase%20by%20alginate-immobilized%20bacteria.pdf text en http://psasir.upm.edu.my/id/eprint/22565/7/38_429.pdf Zakaria, Zainoha and Abdul Razak, Che Nyonya and Ampon, Kamaruzaman and Basri, Mahiran and Wan Yunus, Wan Md. Zin and Shirai, Yoshihito and Salleh, Abu Bakar and Hashimoto, Kenji (1992) Optimum conditions for the production of lipase by alginate-immobilized bacteria. Journal of General and Applied Microbiology, 38 (5). pp. 429-438. ISSN 0022-1260; ESSN: 1349-8037 https://www.jstage.jst.go.jp/article/jgam1955/38/5/38_5_429/_article 10.2323/jgam.38.429 |
| spellingShingle | Zakaria, Zainoha Abdul Razak, Che Nyonya Ampon, Kamaruzaman Basri, Mahiran Wan Yunus, Wan Md. Zin Shirai, Yoshihito Salleh, Abu Bakar Hashimoto, Kenji Optimum conditions for the production of lipase by alginate-immobilized bacteria |
| title | Optimum conditions for the production of lipase by alginate-immobilized bacteria |
| title_full | Optimum conditions for the production of lipase by alginate-immobilized bacteria |
| title_fullStr | Optimum conditions for the production of lipase by alginate-immobilized bacteria |
| title_full_unstemmed | Optimum conditions for the production of lipase by alginate-immobilized bacteria |
| title_short | Optimum conditions for the production of lipase by alginate-immobilized bacteria |
| title_sort | optimum conditions for the production of lipase by alginate immobilized bacteria |
| url | http://psasir.upm.edu.my/id/eprint/22565/1/Optimum%20conditions%20for%20the%20production%20of%20lipase%20by%20alginate-immobilized%20bacteria.pdf http://psasir.upm.edu.my/id/eprint/22565/7/38_429.pdf |
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