Some characteristics of lipases from thermophilic fungi isolated from palm oil mill effluent
Rhizopus oryzae and Rhizopus rhizopodiformis are thermophilic fungi isolated from palm oil mill effluent (POME). They are able to grow up to 50°C and produce remarkable amounts of extracellular lipases. The extracellular lipases from both fungi displayed quite similar characteristics. The optimum pH...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
1997
|
Online Access: | http://psasir.upm.edu.my/id/eprint/22567/1/Some%20characteristics%20of%20lipases%20from%20thermophilic%20fungi%20isolated%20from%20palm%20oil%20mill%20effluent.pdf |
_version_ | 1796970186501259264 |
---|---|
author | Abdul Razak, Che Nyonya Salleh, Abu Bakar Musani, Rosiah Abd. Samad, Mohd. Yusoff Basri, Mahiran |
author_facet | Abdul Razak, Che Nyonya Salleh, Abu Bakar Musani, Rosiah Abd. Samad, Mohd. Yusoff Basri, Mahiran |
author_sort | Abdul Razak, Che Nyonya |
collection | UPM |
description | Rhizopus oryzae and Rhizopus rhizopodiformis are thermophilic fungi isolated from palm oil mill effluent (POME). They are able to grow up to 50°C and produce remarkable amounts of extracellular lipases. The extracellular lipases from both fungi displayed quite similar characteristics. The optimum pH and temperature for both lipases were 6.0 and 45°C, respectively. However, lipase from R. rhizopodiformis was slightly more thermostable than that of R. oryzae lipase but the latter was more stable over broader pH ranges compared to the former, specially at acidic pH. Both the enzyme showed rapid loss of activities at temperatures above 50°C and pH above 7.0. Lipase from R. oryzae showed broader specificities to substrates than R. rhizopodiformis, but both displayed little reactivity for triacetin and polyoxysorbitan (Tween 20, 40, and 60). Both the lipases are 1,3 specific. |
first_indexed | 2024-03-06T07:54:19Z |
format | Article |
id | upm.eprints-22567 |
institution | Universiti Putra Malaysia |
language | English |
last_indexed | 2024-03-06T07:54:19Z |
publishDate | 1997 |
publisher | Elsevier |
record_format | dspace |
spelling | upm.eprints-225672016-02-02T04:06:33Z http://psasir.upm.edu.my/id/eprint/22567/ Some characteristics of lipases from thermophilic fungi isolated from palm oil mill effluent Abdul Razak, Che Nyonya Salleh, Abu Bakar Musani, Rosiah Abd. Samad, Mohd. Yusoff Basri, Mahiran Rhizopus oryzae and Rhizopus rhizopodiformis are thermophilic fungi isolated from palm oil mill effluent (POME). They are able to grow up to 50°C and produce remarkable amounts of extracellular lipases. The extracellular lipases from both fungi displayed quite similar characteristics. The optimum pH and temperature for both lipases were 6.0 and 45°C, respectively. However, lipase from R. rhizopodiformis was slightly more thermostable than that of R. oryzae lipase but the latter was more stable over broader pH ranges compared to the former, specially at acidic pH. Both the enzyme showed rapid loss of activities at temperatures above 50°C and pH above 7.0. Lipase from R. oryzae showed broader specificities to substrates than R. rhizopodiformis, but both displayed little reactivity for triacetin and polyoxysorbitan (Tween 20, 40, and 60). Both the lipases are 1,3 specific. Elsevier 1997-06 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22567/1/Some%20characteristics%20of%20lipases%20from%20thermophilic%20fungi%20isolated%20from%20palm%20oil%20mill%20effluent.pdf Abdul Razak, Che Nyonya and Salleh, Abu Bakar and Musani, Rosiah and Abd. Samad, Mohd. Yusoff and Basri, Mahiran (1997) Some characteristics of lipases from thermophilic fungi isolated from palm oil mill effluent. Journal of Molecular Catalysis B: Enzymatic, 3 (1-4). pp. 153-159. ISSN 1381-1177; ESSN: 1873-3158 http://www.sciencedirect.com/science/article/pii/S1381117796000355 10.1016/S1381-1177(96)00035-5 |
spellingShingle | Abdul Razak, Che Nyonya Salleh, Abu Bakar Musani, Rosiah Abd. Samad, Mohd. Yusoff Basri, Mahiran Some characteristics of lipases from thermophilic fungi isolated from palm oil mill effluent |
title | Some characteristics of lipases from thermophilic fungi isolated from palm oil mill effluent |
title_full | Some characteristics of lipases from thermophilic fungi isolated from palm oil mill effluent |
title_fullStr | Some characteristics of lipases from thermophilic fungi isolated from palm oil mill effluent |
title_full_unstemmed | Some characteristics of lipases from thermophilic fungi isolated from palm oil mill effluent |
title_short | Some characteristics of lipases from thermophilic fungi isolated from palm oil mill effluent |
title_sort | some characteristics of lipases from thermophilic fungi isolated from palm oil mill effluent |
url | http://psasir.upm.edu.my/id/eprint/22567/1/Some%20characteristics%20of%20lipases%20from%20thermophilic%20fungi%20isolated%20from%20palm%20oil%20mill%20effluent.pdf |
work_keys_str_mv | AT abdulrazakchenyonya somecharacteristicsoflipasesfromthermophilicfungiisolatedfrompalmoilmilleffluent AT sallehabubakar somecharacteristicsoflipasesfromthermophilicfungiisolatedfrompalmoilmilleffluent AT musanirosiah somecharacteristicsoflipasesfromthermophilicfungiisolatedfrompalmoilmilleffluent AT abdsamadmohdyusoff somecharacteristicsoflipasesfromthermophilicfungiisolatedfrompalmoilmilleffluent AT basrimahiran somecharacteristicsoflipasesfromthermophilicfungiisolatedfrompalmoilmilleffluent |