Some characteristics of lipases from thermophilic fungi isolated from palm oil mill effluent

Rhizopus oryzae and Rhizopus rhizopodiformis are thermophilic fungi isolated from palm oil mill effluent (POME). They are able to grow up to 50°C and produce remarkable amounts of extracellular lipases. The extracellular lipases from both fungi displayed quite similar characteristics. The optimum pH...

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Main Authors: Abdul Razak, Che Nyonya, Salleh, Abu Bakar, Musani, Rosiah, Abd. Samad, Mohd. Yusoff, Basri, Mahiran
Format: Article
Language:English
Published: Elsevier 1997
Online Access:http://psasir.upm.edu.my/id/eprint/22567/1/Some%20characteristics%20of%20lipases%20from%20thermophilic%20fungi%20isolated%20from%20palm%20oil%20mill%20effluent.pdf
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author Abdul Razak, Che Nyonya
Salleh, Abu Bakar
Musani, Rosiah
Abd. Samad, Mohd. Yusoff
Basri, Mahiran
author_facet Abdul Razak, Che Nyonya
Salleh, Abu Bakar
Musani, Rosiah
Abd. Samad, Mohd. Yusoff
Basri, Mahiran
author_sort Abdul Razak, Che Nyonya
collection UPM
description Rhizopus oryzae and Rhizopus rhizopodiformis are thermophilic fungi isolated from palm oil mill effluent (POME). They are able to grow up to 50°C and produce remarkable amounts of extracellular lipases. The extracellular lipases from both fungi displayed quite similar characteristics. The optimum pH and temperature for both lipases were 6.0 and 45°C, respectively. However, lipase from R. rhizopodiformis was slightly more thermostable than that of R. oryzae lipase but the latter was more stable over broader pH ranges compared to the former, specially at acidic pH. Both the enzyme showed rapid loss of activities at temperatures above 50°C and pH above 7.0. Lipase from R. oryzae showed broader specificities to substrates than R. rhizopodiformis, but both displayed little reactivity for triacetin and polyoxysorbitan (Tween 20, 40, and 60). Both the lipases are 1,3 specific.
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spelling upm.eprints-225672016-02-02T04:06:33Z http://psasir.upm.edu.my/id/eprint/22567/ Some characteristics of lipases from thermophilic fungi isolated from palm oil mill effluent Abdul Razak, Che Nyonya Salleh, Abu Bakar Musani, Rosiah Abd. Samad, Mohd. Yusoff Basri, Mahiran Rhizopus oryzae and Rhizopus rhizopodiformis are thermophilic fungi isolated from palm oil mill effluent (POME). They are able to grow up to 50°C and produce remarkable amounts of extracellular lipases. The extracellular lipases from both fungi displayed quite similar characteristics. The optimum pH and temperature for both lipases were 6.0 and 45°C, respectively. However, lipase from R. rhizopodiformis was slightly more thermostable than that of R. oryzae lipase but the latter was more stable over broader pH ranges compared to the former, specially at acidic pH. Both the enzyme showed rapid loss of activities at temperatures above 50°C and pH above 7.0. Lipase from R. oryzae showed broader specificities to substrates than R. rhizopodiformis, but both displayed little reactivity for triacetin and polyoxysorbitan (Tween 20, 40, and 60). Both the lipases are 1,3 specific. Elsevier 1997-06 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22567/1/Some%20characteristics%20of%20lipases%20from%20thermophilic%20fungi%20isolated%20from%20palm%20oil%20mill%20effluent.pdf Abdul Razak, Che Nyonya and Salleh, Abu Bakar and Musani, Rosiah and Abd. Samad, Mohd. Yusoff and Basri, Mahiran (1997) Some characteristics of lipases from thermophilic fungi isolated from palm oil mill effluent. Journal of Molecular Catalysis B: Enzymatic, 3 (1-4). pp. 153-159. ISSN 1381-1177; ESSN: 1873-3158 http://www.sciencedirect.com/science/article/pii/S1381117796000355 10.1016/S1381-1177(96)00035-5
spellingShingle Abdul Razak, Che Nyonya
Salleh, Abu Bakar
Musani, Rosiah
Abd. Samad, Mohd. Yusoff
Basri, Mahiran
Some characteristics of lipases from thermophilic fungi isolated from palm oil mill effluent
title Some characteristics of lipases from thermophilic fungi isolated from palm oil mill effluent
title_full Some characteristics of lipases from thermophilic fungi isolated from palm oil mill effluent
title_fullStr Some characteristics of lipases from thermophilic fungi isolated from palm oil mill effluent
title_full_unstemmed Some characteristics of lipases from thermophilic fungi isolated from palm oil mill effluent
title_short Some characteristics of lipases from thermophilic fungi isolated from palm oil mill effluent
title_sort some characteristics of lipases from thermophilic fungi isolated from palm oil mill effluent
url http://psasir.upm.edu.my/id/eprint/22567/1/Some%20characteristics%20of%20lipases%20from%20thermophilic%20fungi%20isolated%20from%20palm%20oil%20mill%20effluent.pdf
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