Extra- and intra-cellular lipases from a thermophilic Rhizopus oryzae and factors affecting their production
A thermophilic Rhizopus oryzae was isolated, and parameters affecting its production of extra- and intra-cellular lipases were investigated. All carbon sources tested with the exception of sucrose generally inhibited the production of extracellular lipase, but enhanced the production of intracellula...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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NRC Research Press
1993
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22568/1/Extra-%20and%20intra-cellular%20lipases%20from%20a%20thermophilic%20Rhizopus%20oryzae%20and%20factors%20affecting%20their%20production.pdf |
| _version_ | 1796970186708877312 |
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| author | Salleh, Abu Bakar Musani, Rosiah Basri, Mahiran Ampon, Kamaruzaman Wan Yunus, Wan Md. Zin Abdul Razak, Che Nyonya |
| author_facet | Salleh, Abu Bakar Musani, Rosiah Basri, Mahiran Ampon, Kamaruzaman Wan Yunus, Wan Md. Zin Abdul Razak, Che Nyonya |
| author_sort | Salleh, Abu Bakar |
| collection | UPM |
| description | A thermophilic Rhizopus oryzae was isolated, and parameters affecting its production of extra- and intra-cellular lipases were investigated. All carbon sources tested with the exception of sucrose generally inhibited the production of extracellular lipase, but enhanced the production of intracellular lipase. Peptone was the best substrate for extracellular enzyme production, but for intracellular lipase production other substrates such as tryptone, tryptic soy digest, polypeptone, and corn steep liquor gave comparable results. Among lipid substrates, glycerol was the only stimulator of extracellular enzyme production, whereas olive oil, triolein, and oleic acid had very positive effects on intracellular enzyme production. Shaking enhanced the production of both types of enzymes; the temperature optima were 45 and 37°C for extra- and intra-cellular lipases, respectively. A pH of 5.0 was optimal for production of both enzymes. |
| first_indexed | 2024-03-06T07:54:19Z |
| format | Article |
| id | upm.eprints-22568 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:54:19Z |
| publishDate | 1993 |
| publisher | NRC Research Press |
| record_format | dspace |
| spelling | upm.eprints-225682016-02-02T04:06:13Z http://psasir.upm.edu.my/id/eprint/22568/ Extra- and intra-cellular lipases from a thermophilic Rhizopus oryzae and factors affecting their production Salleh, Abu Bakar Musani, Rosiah Basri, Mahiran Ampon, Kamaruzaman Wan Yunus, Wan Md. Zin Abdul Razak, Che Nyonya A thermophilic Rhizopus oryzae was isolated, and parameters affecting its production of extra- and intra-cellular lipases were investigated. All carbon sources tested with the exception of sucrose generally inhibited the production of extracellular lipase, but enhanced the production of intracellular lipase. Peptone was the best substrate for extracellular enzyme production, but for intracellular lipase production other substrates such as tryptone, tryptic soy digest, polypeptone, and corn steep liquor gave comparable results. Among lipid substrates, glycerol was the only stimulator of extracellular enzyme production, whereas olive oil, triolein, and oleic acid had very positive effects on intracellular enzyme production. Shaking enhanced the production of both types of enzymes; the temperature optima were 45 and 37°C for extra- and intra-cellular lipases, respectively. A pH of 5.0 was optimal for production of both enzymes. NRC Research Press 1993 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22568/1/Extra-%20and%20intra-cellular%20lipases%20from%20a%20thermophilic%20Rhizopus%20oryzae%20and%20factors%20affecting%20their%20production.pdf Salleh, Abu Bakar and Musani, Rosiah and Basri, Mahiran and Ampon, Kamaruzaman and Wan Yunus, Wan Md. Zin and Abdul Razak, Che Nyonya (1993) Extra- and intra-cellular lipases from a thermophilic Rhizopus oryzae and factors affecting their production. Canadian Journal of Microbiology, 39 (10). pp. 978-981. ISSN 0008-4166; ESSN: 1480-3275 http://www.nrcresearchpress.com/doi/abs/10.1139/m93-147?journalCode=cjm#.VqB2XlJrsZM 10.1139/m93-147 |
| spellingShingle | Salleh, Abu Bakar Musani, Rosiah Basri, Mahiran Ampon, Kamaruzaman Wan Yunus, Wan Md. Zin Abdul Razak, Che Nyonya Extra- and intra-cellular lipases from a thermophilic Rhizopus oryzae and factors affecting their production |
| title | Extra- and intra-cellular lipases from a thermophilic Rhizopus oryzae and factors affecting their production |
| title_full | Extra- and intra-cellular lipases from a thermophilic Rhizopus oryzae and factors affecting their production |
| title_fullStr | Extra- and intra-cellular lipases from a thermophilic Rhizopus oryzae and factors affecting their production |
| title_full_unstemmed | Extra- and intra-cellular lipases from a thermophilic Rhizopus oryzae and factors affecting their production |
| title_short | Extra- and intra-cellular lipases from a thermophilic Rhizopus oryzae and factors affecting their production |
| title_sort | extra and intra cellular lipases from a thermophilic rhizopus oryzae and factors affecting their production |
| url | http://psasir.upm.edu.my/id/eprint/22568/1/Extra-%20and%20intra-cellular%20lipases%20from%20a%20thermophilic%20Rhizopus%20oryzae%20and%20factors%20affecting%20their%20production.pdf |
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