LC-MS/MS-based metabolites of Eurycoma longifolia (Tongkat Ali) in Malaysia (Perak and Pahang).
A number of three LC–MS/MS hybrid systems (QTof, TripleTof and QTrap) has been used to profile small metabolites (m/z 100–1000) and to detect the targeted metabolites such as quassinoids, alkaloids, triterpene and biphenylneolignans from the aqueous extracts of Eurycoma longifolia. The metabolite pr...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English |
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2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/23304/1/LC.pdf |
| _version_ | 1796970332950626304 |
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| author | Mohd Amin, Nor Amaiza Chua, Lee Suan Chun, Jason Hong Neo Ting, Hun Lee Sarmidi, Mohamad Roji Abdul Aziz, Ramlan |
| author_facet | Mohd Amin, Nor Amaiza Chua, Lee Suan Chun, Jason Hong Neo Ting, Hun Lee Sarmidi, Mohamad Roji Abdul Aziz, Ramlan |
| author_sort | Mohd Amin, Nor Amaiza |
| collection | UPM |
| description | A number of three LC–MS/MS hybrid systems (QTof, TripleTof and QTrap) has been used to profile small metabolites (m/z 100–1000) and to detect the targeted metabolites such as quassinoids, alkaloids, triterpene and biphenylneolignans from the aqueous extracts of Eurycoma longifolia. The metabolite profiles of small molecules showed four significant clusters in the principle component analysis for the aqueous extracts of E. longifolia, which had been collected from different geographical terrains (Perak and Pahang) and processed at different extraction temperatures (35 °C and 100 °C). A small peptide of leucine (m/z 679) and a new hydroxyl methyl β-carboline propionic acid have been identified to differentiate E. longifolia extracts that prepared at 35 °C and 100 °C, respectively. From the targeted metabolites identification, it was found that 3,4ɛ-dihydroeurycomanone (quassinoids) and eurylene (squalene-type triterpene) could only be detected in the Pahang extract, whereas canthin-6-one-3N-oxide could only be detected in the Perak extract. Overall, quassinoids were present in the highest concentration, particularly eurycomanone and its derivatives compared to the other groups of metabolites. However, the concentration of canthin-6-one and β-carboline alkaloids was significantly increased when the roots of the plant samples were extracted at 100 °C. |
| first_indexed | 2024-03-06T07:56:29Z |
| format | Article |
| id | upm.eprints-23304 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:56:29Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | upm.eprints-233042015-10-20T00:19:52Z http://psasir.upm.edu.my/id/eprint/23304/ LC-MS/MS-based metabolites of Eurycoma longifolia (Tongkat Ali) in Malaysia (Perak and Pahang). Mohd Amin, Nor Amaiza Chua, Lee Suan Chun, Jason Hong Neo Ting, Hun Lee Sarmidi, Mohamad Roji Abdul Aziz, Ramlan A number of three LC–MS/MS hybrid systems (QTof, TripleTof and QTrap) has been used to profile small metabolites (m/z 100–1000) and to detect the targeted metabolites such as quassinoids, alkaloids, triterpene and biphenylneolignans from the aqueous extracts of Eurycoma longifolia. The metabolite profiles of small molecules showed four significant clusters in the principle component analysis for the aqueous extracts of E. longifolia, which had been collected from different geographical terrains (Perak and Pahang) and processed at different extraction temperatures (35 °C and 100 °C). A small peptide of leucine (m/z 679) and a new hydroxyl methyl β-carboline propionic acid have been identified to differentiate E. longifolia extracts that prepared at 35 °C and 100 °C, respectively. From the targeted metabolites identification, it was found that 3,4ɛ-dihydroeurycomanone (quassinoids) and eurylene (squalene-type triterpene) could only be detected in the Pahang extract, whereas canthin-6-one-3N-oxide could only be detected in the Perak extract. Overall, quassinoids were present in the highest concentration, particularly eurycomanone and its derivatives compared to the other groups of metabolites. However, the concentration of canthin-6-one and β-carboline alkaloids was significantly increased when the roots of the plant samples were extracted at 100 °C. 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/23304/1/LC.pdf Mohd Amin, Nor Amaiza and Chua, Lee Suan and Chun, Jason Hong Neo and Ting, Hun Lee and Sarmidi, Mohamad Roji and Abdul Aziz, Ramlan (2011) LC-MS/MS-based metabolites of Eurycoma longifolia (Tongkat Ali) in Malaysia (Perak and Pahang). Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 879 (32). pp. 390-391. ISSN 1570-0232 10.1016/j.jchromb.2011.11.002 English |
| spellingShingle | Mohd Amin, Nor Amaiza Chua, Lee Suan Chun, Jason Hong Neo Ting, Hun Lee Sarmidi, Mohamad Roji Abdul Aziz, Ramlan LC-MS/MS-based metabolites of Eurycoma longifolia (Tongkat Ali) in Malaysia (Perak and Pahang). |
| title | LC-MS/MS-based metabolites of Eurycoma longifolia (Tongkat Ali) in Malaysia (Perak and Pahang). |
| title_full | LC-MS/MS-based metabolites of Eurycoma longifolia (Tongkat Ali) in Malaysia (Perak and Pahang). |
| title_fullStr | LC-MS/MS-based metabolites of Eurycoma longifolia (Tongkat Ali) in Malaysia (Perak and Pahang). |
| title_full_unstemmed | LC-MS/MS-based metabolites of Eurycoma longifolia (Tongkat Ali) in Malaysia (Perak and Pahang). |
| title_short | LC-MS/MS-based metabolites of Eurycoma longifolia (Tongkat Ali) in Malaysia (Perak and Pahang). |
| title_sort | lc ms ms based metabolites of eurycoma longifolia tongkat ali in malaysia perak and pahang |
| url | http://psasir.upm.edu.my/id/eprint/23304/1/LC.pdf |
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