Fundamental concept of cryopreservation using Dendrobium sonia-17 protocorm-like bodies by encapsulation-dehydration technique
This study was carried out to evaluate the potential of using the encapsulation-dehydration technique on cryopreservation protocorm-like bodies (PLBs) of Dendrobium sonia-17. The survival of the PLBs was assessed based on the effects of 4 dehydration periods (0, 1, 3 and 5 h) and 4 different concent...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Academic Journals
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/23682/1/23682.pdf |
| _version_ | 1796970408778399744 |
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| author | Subramaniam, Sreeramanan Sinniah, Uma Rani Khoddamzadeh, Amir Ali Periasamy, Suranthran James Antony, Jessica Jeyanthi |
| author_facet | Subramaniam, Sreeramanan Sinniah, Uma Rani Khoddamzadeh, Amir Ali Periasamy, Suranthran James Antony, Jessica Jeyanthi |
| author_sort | Subramaniam, Sreeramanan |
| collection | UPM |
| description | This study was carried out to evaluate the potential of using the encapsulation-dehydration technique on cryopreservation protocorm-like bodies (PLBs) of Dendrobium sonia-17. The survival of the PLBs was assessed based on the effects of 4 dehydration periods (0, 1, 3 and 5 h) and 4 different concentrations of 24-h sucrose pretreatment (0, 0.3, 0.5 and 0.7 M). Upon dehydration, moisture content was determined and the PLBs were evaluated for survival using absorbance values from 2, 3,5- triphenyltetrazolium chloride (TTC) assay at 530 nm and regeneration observations. Moisture content declined with the dehydration time, but the decline was not significant for encapsulated PLBs. All cryopreserved PLBs gave very low survival irrespective of the dehydration period. The best survival percentage in the cryopreservation of the PLBs of Dendrobium sonia-17 was obtained when the combination of 0.5 M sucrose pretreatment and 3 h dehydration time was applied in the experiment. |
| first_indexed | 2024-03-06T07:57:36Z |
| format | Article |
| id | upm.eprints-23682 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:57:36Z |
| publishDate | 2011 |
| publisher | Academic Journals |
| record_format | dspace |
| spelling | upm.eprints-236822016-05-31T07:55:19Z http://psasir.upm.edu.my/id/eprint/23682/ Fundamental concept of cryopreservation using Dendrobium sonia-17 protocorm-like bodies by encapsulation-dehydration technique Subramaniam, Sreeramanan Sinniah, Uma Rani Khoddamzadeh, Amir Ali Periasamy, Suranthran James Antony, Jessica Jeyanthi This study was carried out to evaluate the potential of using the encapsulation-dehydration technique on cryopreservation protocorm-like bodies (PLBs) of Dendrobium sonia-17. The survival of the PLBs was assessed based on the effects of 4 dehydration periods (0, 1, 3 and 5 h) and 4 different concentrations of 24-h sucrose pretreatment (0, 0.3, 0.5 and 0.7 M). Upon dehydration, moisture content was determined and the PLBs were evaluated for survival using absorbance values from 2, 3,5- triphenyltetrazolium chloride (TTC) assay at 530 nm and regeneration observations. Moisture content declined with the dehydration time, but the decline was not significant for encapsulated PLBs. All cryopreserved PLBs gave very low survival irrespective of the dehydration period. The best survival percentage in the cryopreservation of the PLBs of Dendrobium sonia-17 was obtained when the combination of 0.5 M sucrose pretreatment and 3 h dehydration time was applied in the experiment. Academic Journals 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/23682/1/23682.pdf Subramaniam, Sreeramanan and Sinniah, Uma Rani and Khoddamzadeh, Amir Ali and Periasamy, Suranthran and James Antony, Jessica Jeyanthi (2011) Fundamental concept of cryopreservation using Dendrobium sonia-17 protocorm-like bodies by encapsulation-dehydration technique. African Journal of Biotechnology, 10 (19). art. no. 17DD02527764. pp. 3902-3907. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/17DD02527764 10.5897/AJB10.2265 |
| spellingShingle | Subramaniam, Sreeramanan Sinniah, Uma Rani Khoddamzadeh, Amir Ali Periasamy, Suranthran James Antony, Jessica Jeyanthi Fundamental concept of cryopreservation using Dendrobium sonia-17 protocorm-like bodies by encapsulation-dehydration technique |
| title | Fundamental concept of cryopreservation using Dendrobium sonia-17 protocorm-like bodies by encapsulation-dehydration technique |
| title_full | Fundamental concept of cryopreservation using Dendrobium sonia-17 protocorm-like bodies by encapsulation-dehydration technique |
| title_fullStr | Fundamental concept of cryopreservation using Dendrobium sonia-17 protocorm-like bodies by encapsulation-dehydration technique |
| title_full_unstemmed | Fundamental concept of cryopreservation using Dendrobium sonia-17 protocorm-like bodies by encapsulation-dehydration technique |
| title_short | Fundamental concept of cryopreservation using Dendrobium sonia-17 protocorm-like bodies by encapsulation-dehydration technique |
| title_sort | fundamental concept of cryopreservation using dendrobium sonia 17 protocorm like bodies by encapsulation dehydration technique |
| url | http://psasir.upm.edu.my/id/eprint/23682/1/23682.pdf |
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