First report of cabbage soft rot caused by Pectobacterium carotovorum subsp carotovorum in Malaysia.

Cabbage plants with symptoms of soft rot were sampled from commercial fields in Malaysia during the winter of 2010. Disease symptoms were a grey to pale brown discoloration and expanding water-soaked lesions on leaves. Several cabbage fields producing white cultivars were investigated and 27 samples...

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Main Authors: Nazerian, Eisa, Sijam , Kamaruzaman, Mior Ahmad, Zainal Abidin, Vadamalai, Ganesan
Format: Article
Language:English
English
Published: American Phytopathological Society (APS Press) 2011
Online Access:http://psasir.upm.edu.my/id/eprint/23721/1/First%20report%20of%20cabbage%20soft%20rot%20caused%20by%20Pectobacterium%20carotovorum%20subsp%20carotovorum%20in%20Malaysia.pdf
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author Nazerian, Eisa
Sijam , Kamaruzaman
Mior Ahmad, Zainal Abidin
Vadamalai, Ganesan
author_facet Nazerian, Eisa
Sijam , Kamaruzaman
Mior Ahmad, Zainal Abidin
Vadamalai, Ganesan
author_sort Nazerian, Eisa
collection UPM
description Cabbage plants with symptoms of soft rot were sampled from commercial fields in Malaysia during the winter of 2010. Disease symptoms were a grey to pale brown discoloration and expanding water-soaked lesions on leaves. Several cabbage fields producing white cultivars were investigated and 27 samples were collected. The bacteria reisolated from rotted cabbage slices resembled P. carotovorum cultural characteristics and could cause soft rot in subsequent tests. PCR amplification with Y1 and Y2 primers, which are specific for P. carotovorum, produced a 434-bp band with 15 strains. Analysis by ITS-PCR and ITS-restriction fragment length polymorphism identified all the isolates as P. carotovorum subsp. carotovorum. This is thought to be the first report of P. carotovorum subsp. carotovorum in cabbage from Malaysia.
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spelling upm.eprints-237212015-12-16T04:21:08Z http://psasir.upm.edu.my/id/eprint/23721/ First report of cabbage soft rot caused by Pectobacterium carotovorum subsp carotovorum in Malaysia. Nazerian, Eisa Sijam , Kamaruzaman Mior Ahmad, Zainal Abidin Vadamalai, Ganesan Cabbage plants with symptoms of soft rot were sampled from commercial fields in Malaysia during the winter of 2010. Disease symptoms were a grey to pale brown discoloration and expanding water-soaked lesions on leaves. Several cabbage fields producing white cultivars were investigated and 27 samples were collected. The bacteria reisolated from rotted cabbage slices resembled P. carotovorum cultural characteristics and could cause soft rot in subsequent tests. PCR amplification with Y1 and Y2 primers, which are specific for P. carotovorum, produced a 434-bp band with 15 strains. Analysis by ITS-PCR and ITS-restriction fragment length polymorphism identified all the isolates as P. carotovorum subsp. carotovorum. This is thought to be the first report of P. carotovorum subsp. carotovorum in cabbage from Malaysia. American Phytopathological Society (APS Press) 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/23721/1/First%20report%20of%20cabbage%20soft%20rot%20caused%20by%20Pectobacterium%20carotovorum%20subsp%20carotovorum%20in%20Malaysia.pdf Nazerian, Eisa and Sijam , Kamaruzaman and Mior Ahmad, Zainal Abidin and Vadamalai, Ganesan (2011) First report of cabbage soft rot caused by Pectobacterium carotovorum subsp carotovorum in Malaysia. Plant Disease, 95 (4). p. 491. ISSN 0191-2917 English
spellingShingle Nazerian, Eisa
Sijam , Kamaruzaman
Mior Ahmad, Zainal Abidin
Vadamalai, Ganesan
First report of cabbage soft rot caused by Pectobacterium carotovorum subsp carotovorum in Malaysia.
title First report of cabbage soft rot caused by Pectobacterium carotovorum subsp carotovorum in Malaysia.
title_full First report of cabbage soft rot caused by Pectobacterium carotovorum subsp carotovorum in Malaysia.
title_fullStr First report of cabbage soft rot caused by Pectobacterium carotovorum subsp carotovorum in Malaysia.
title_full_unstemmed First report of cabbage soft rot caused by Pectobacterium carotovorum subsp carotovorum in Malaysia.
title_short First report of cabbage soft rot caused by Pectobacterium carotovorum subsp carotovorum in Malaysia.
title_sort first report of cabbage soft rot caused by pectobacterium carotovorum subsp carotovorum in malaysia
url http://psasir.upm.edu.my/id/eprint/23721/1/First%20report%20of%20cabbage%20soft%20rot%20caused%20by%20Pectobacterium%20carotovorum%20subsp%20carotovorum%20in%20Malaysia.pdf
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