Characterization of Pectobacterium carotovorum causing a new soft rot disease on okra in Malaysia.
Dark brown, necrotic pods with extensive water-soaked lesions caused by plant pathogenic bacteria were found on okra plants in different fields in Malaysia in 2010. PCR amplification of the pectate lyase (pel) gene and amplification of the 16S–23S rRNA (ITS) with G1 and L1 primers produced 434-, 535...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
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Springer
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/23725/1/Characterization%20of%20Pectobacterium%20carotovorum%20causing%20a%20new%20soft%20rot%20disease%20on%20okra%20in%20Malaysia.pdf |
| _version_ | 1796970417246699520 |
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| author | Nazerian, Eisa Sijam, Kamaruzaman Mior Ahmad, Zainal Abidin Keshavarz, Kavous |
| author_facet | Nazerian, Eisa Sijam, Kamaruzaman Mior Ahmad, Zainal Abidin Keshavarz, Kavous |
| author_sort | Nazerian, Eisa |
| collection | UPM |
| description | Dark brown, necrotic pods with extensive water-soaked lesions caused by plant pathogenic bacteria were found on okra plants in different fields in Malaysia in 2010. PCR amplification of the pectate lyase (pel) gene and amplification of the 16S–23S rRNA (ITS) with G1 and L1 primers produced 434-, 535- and 570-bp fragments, respectively. From the similarity between the results of biochemical tests and their equivalency with standard bacteriological sources, PCR-based pel gene, and RFLP analysis of the ITS-PCR products, all isolates were identified as Pectobacterium carotovorum. This is the first report of P. carotovorum in okra from Malaysia. |
| first_indexed | 2024-03-06T07:57:44Z |
| format | Article |
| id | upm.eprints-23725 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:57:44Z |
| publishDate | 2011 |
| publisher | Springer |
| record_format | dspace |
| spelling | upm.eprints-237252015-12-10T03:38:17Z http://psasir.upm.edu.my/id/eprint/23725/ Characterization of Pectobacterium carotovorum causing a new soft rot disease on okra in Malaysia. Nazerian, Eisa Sijam, Kamaruzaman Mior Ahmad, Zainal Abidin Keshavarz, Kavous Dark brown, necrotic pods with extensive water-soaked lesions caused by plant pathogenic bacteria were found on okra plants in different fields in Malaysia in 2010. PCR amplification of the pectate lyase (pel) gene and amplification of the 16S–23S rRNA (ITS) with G1 and L1 primers produced 434-, 535- and 570-bp fragments, respectively. From the similarity between the results of biochemical tests and their equivalency with standard bacteriological sources, PCR-based pel gene, and RFLP analysis of the ITS-PCR products, all isolates were identified as Pectobacterium carotovorum. This is the first report of P. carotovorum in okra from Malaysia. Springer 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/23725/1/Characterization%20of%20Pectobacterium%20carotovorum%20causing%20a%20new%20soft%20rot%20disease%20on%20okra%20in%20Malaysia.pdf Nazerian, Eisa and Sijam, Kamaruzaman and Mior Ahmad, Zainal Abidin and Keshavarz, Kavous (2011) Characterization of Pectobacterium carotovorum causing a new soft rot disease on okra in Malaysia. Journal of General Plant Pathology, 77 (5). pp. 292-294. ISSN 1345-2630; ESSN: 1610-739X 10.1007/s10327-011-0322-1 English |
| spellingShingle | Nazerian, Eisa Sijam, Kamaruzaman Mior Ahmad, Zainal Abidin Keshavarz, Kavous Characterization of Pectobacterium carotovorum causing a new soft rot disease on okra in Malaysia. |
| title | Characterization of Pectobacterium carotovorum causing a new soft rot disease on okra in Malaysia. |
| title_full | Characterization of Pectobacterium carotovorum causing a new soft rot disease on okra in Malaysia. |
| title_fullStr | Characterization of Pectobacterium carotovorum causing a new soft rot disease on okra in Malaysia. |
| title_full_unstemmed | Characterization of Pectobacterium carotovorum causing a new soft rot disease on okra in Malaysia. |
| title_short | Characterization of Pectobacterium carotovorum causing a new soft rot disease on okra in Malaysia. |
| title_sort | characterization of pectobacterium carotovorum causing a new soft rot disease on okra in malaysia |
| url | http://psasir.upm.edu.my/id/eprint/23725/1/Characterization%20of%20Pectobacterium%20carotovorum%20causing%20a%20new%20soft%20rot%20disease%20on%20okra%20in%20Malaysia.pdf |
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