In vitro plant regeneration from protocorms-like bodies (PLBs) and callus of Phalaenopsis gigantea (Epidendroideae: Orchidaceae)
Phalaenopsis, with long arching sprays of flowers, are among the most beautiful flowers in the world. Phalaenopsis is an important genus and one of the most popular epiphytic monopodial orchids, grown commercially for the production of cut flowers and potted plants. Most of them have different and i...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Academic Journals
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/23770/1/23770.pdf |
| _version_ | 1825925428484767744 |
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| author | Niknejad, Azadeh Abdul Kadir, Mihdzar Kadzimin, Saleh |
| author_facet | Niknejad, Azadeh Abdul Kadir, Mihdzar Kadzimin, Saleh |
| author_sort | Niknejad, Azadeh |
| collection | UPM |
| description | Phalaenopsis, with long arching sprays of flowers, are among the most beautiful flowers in the world. Phalaenopsis is an important genus and one of the most popular epiphytic monopodial orchids, grown commercially for the production of cut flowers and potted plants. Most of them have different and interesting morphological characteristics which have different value to the breeders. Phalaenopsis gigantea is one of the most difficult to grow and has the potential of producing beautiful hybrids. An efficient and reproducible method for large-scale propagation of Ph. giganteausing leaf sections has been developed. Leaf sections from in vitro young plants were cultured on New Dogashima Medium (NDM) supplemented with cytokinins (6-Benzylaminopurine (BAP), Thidiazuron (TDZ), and Kinetin (KIN), each at 0.01, 0.1, 0.5 and 1.0 mg/L) alone and in combinations with (auxins a-naphthaleneacetic acid (NAA), at 0.01, 0.1, 0.5 and 1.0 mg/L). The explants developed calli and protocorm-like-bodies (PLBs) within 6 weeks of culture. Treatment TDZ in combination with auxins was found to be the best for the induction of callus and PLBs. In vitroregeneration of Ph. gigantea PLB was achieved by exposure to light and transferring to hormone free NDM solid medium. |
| first_indexed | 2024-03-06T07:57:52Z |
| format | Article |
| id | upm.eprints-23770 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:57:52Z |
| publishDate | 2011 |
| publisher | Academic Journals |
| record_format | dspace |
| spelling | upm.eprints-237702017-11-14T08:34:15Z http://psasir.upm.edu.my/id/eprint/23770/ In vitro plant regeneration from protocorms-like bodies (PLBs) and callus of Phalaenopsis gigantea (Epidendroideae: Orchidaceae) Niknejad, Azadeh Abdul Kadir, Mihdzar Kadzimin, Saleh Phalaenopsis, with long arching sprays of flowers, are among the most beautiful flowers in the world. Phalaenopsis is an important genus and one of the most popular epiphytic monopodial orchids, grown commercially for the production of cut flowers and potted plants. Most of them have different and interesting morphological characteristics which have different value to the breeders. Phalaenopsis gigantea is one of the most difficult to grow and has the potential of producing beautiful hybrids. An efficient and reproducible method for large-scale propagation of Ph. giganteausing leaf sections has been developed. Leaf sections from in vitro young plants were cultured on New Dogashima Medium (NDM) supplemented with cytokinins (6-Benzylaminopurine (BAP), Thidiazuron (TDZ), and Kinetin (KIN), each at 0.01, 0.1, 0.5 and 1.0 mg/L) alone and in combinations with (auxins a-naphthaleneacetic acid (NAA), at 0.01, 0.1, 0.5 and 1.0 mg/L). The explants developed calli and protocorm-like-bodies (PLBs) within 6 weeks of culture. Treatment TDZ in combination with auxins was found to be the best for the induction of callus and PLBs. In vitroregeneration of Ph. gigantea PLB was achieved by exposure to light and transferring to hormone free NDM solid medium. Academic Journals 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/23770/1/23770.pdf Niknejad, Azadeh and Abdul Kadir, Mihdzar and Kadzimin, Saleh (2011) In vitro plant regeneration from protocorms-like bodies (PLBs) and callus of Phalaenopsis gigantea (Epidendroideae: Orchidaceae). African Journal of Biotechnology, 10 (56). art. no. BBDEBF635675. pp. 11808-11816. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/BBDEBF635675 |
| spellingShingle | Niknejad, Azadeh Abdul Kadir, Mihdzar Kadzimin, Saleh In vitro plant regeneration from protocorms-like bodies (PLBs) and callus of Phalaenopsis gigantea (Epidendroideae: Orchidaceae) |
| title | In vitro plant regeneration from protocorms-like bodies (PLBs) and callus of Phalaenopsis gigantea (Epidendroideae: Orchidaceae) |
| title_full | In vitro plant regeneration from protocorms-like bodies (PLBs) and callus of Phalaenopsis gigantea (Epidendroideae: Orchidaceae) |
| title_fullStr | In vitro plant regeneration from protocorms-like bodies (PLBs) and callus of Phalaenopsis gigantea (Epidendroideae: Orchidaceae) |
| title_full_unstemmed | In vitro plant regeneration from protocorms-like bodies (PLBs) and callus of Phalaenopsis gigantea (Epidendroideae: Orchidaceae) |
| title_short | In vitro plant regeneration from protocorms-like bodies (PLBs) and callus of Phalaenopsis gigantea (Epidendroideae: Orchidaceae) |
| title_sort | in vitro plant regeneration from protocorms like bodies plbs and callus of phalaenopsis gigantea epidendroideae orchidaceae |
| url | http://psasir.upm.edu.my/id/eprint/23770/1/23770.pdf |
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