In vitro adventitious shoot regeneration and acclimatisation of Brassica oleracea subsp. italica cv. Green Marvel
Cotyledonary explants of Brassica oleracea subsp. italica (broccoli) cv. Green Marvel were cultured on Murashige and Skoog (MS) medium containing different combinations of the growth regulators 6- benzylaminopurine (BAP) and α-naphthalene acetic acid (NAA) for shoot regeneration. The optimal medium...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Academic Journals
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/23775/1/23775.pdf |
| _version_ | 1825925429555363840 |
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| author | Rafanfar, Seyed Ali Abdul Aziz, Maheran Abdul Kadir, Mihdzar Abdul Rashid, Azmi Haddadi, Fatemeh |
| author_facet | Rafanfar, Seyed Ali Abdul Aziz, Maheran Abdul Kadir, Mihdzar Abdul Rashid, Azmi Haddadi, Fatemeh |
| author_sort | Rafanfar, Seyed Ali |
| collection | UPM |
| description | Cotyledonary explants of Brassica oleracea subsp. italica (broccoli) cv. Green Marvel were cultured on Murashige and Skoog (MS) medium containing different combinations of the growth regulators 6- benzylaminopurine (BAP) and α-naphthalene acetic acid (NAA) for shoot regeneration. The optimal medium for inducing shoots contained 3 mgl-1 BAP and 1 mgl-1 NAA, which produced a shoot induction percentage of 53.33% and a mean number of 0.43 shoot per explant. The shoots were subsequently rooted in MS medium that contained 0.2 mgl-1 of indol-3-butyric acid (IBA). Different potting media were assessed during plantlet acclimatization. The highest percentage of plant survival (83.33%) was on the medium that contained sand and soil (1:1), while maximum root length (4.37 cm) and plant height (7.87 cm) were attained in potting medium that consisted peat moss, perlite and vermiculite (3:1:1). |
| first_indexed | 2024-03-06T07:57:53Z |
| format | Article |
| id | upm.eprints-23775 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:57:53Z |
| publishDate | 2011 |
| publisher | Academic Journals |
| record_format | dspace |
| spelling | upm.eprints-237752017-11-14T03:14:58Z http://psasir.upm.edu.my/id/eprint/23775/ In vitro adventitious shoot regeneration and acclimatisation of Brassica oleracea subsp. italica cv. Green Marvel Rafanfar, Seyed Ali Abdul Aziz, Maheran Abdul Kadir, Mihdzar Abdul Rashid, Azmi Haddadi, Fatemeh Cotyledonary explants of Brassica oleracea subsp. italica (broccoli) cv. Green Marvel were cultured on Murashige and Skoog (MS) medium containing different combinations of the growth regulators 6- benzylaminopurine (BAP) and α-naphthalene acetic acid (NAA) for shoot regeneration. The optimal medium for inducing shoots contained 3 mgl-1 BAP and 1 mgl-1 NAA, which produced a shoot induction percentage of 53.33% and a mean number of 0.43 shoot per explant. The shoots were subsequently rooted in MS medium that contained 0.2 mgl-1 of indol-3-butyric acid (IBA). Different potting media were assessed during plantlet acclimatization. The highest percentage of plant survival (83.33%) was on the medium that contained sand and soil (1:1), while maximum root length (4.37 cm) and plant height (7.87 cm) were attained in potting medium that consisted peat moss, perlite and vermiculite (3:1:1). Academic Journals 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/23775/1/23775.pdf Rafanfar, Seyed Ali and Abdul Aziz, Maheran and Abdul Kadir, Mihdzar and Abdul Rashid, Azmi and Haddadi, Fatemeh (2011) In vitro adventitious shoot regeneration and acclimatisation of Brassica oleracea subsp. italica cv. Green Marvel. African Journal of Biotechnology, 10 (29). art. no. 1B4311E35828. pp. 5614-5619. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/1B4311E35828 |
| spellingShingle | Rafanfar, Seyed Ali Abdul Aziz, Maheran Abdul Kadir, Mihdzar Abdul Rashid, Azmi Haddadi, Fatemeh In vitro adventitious shoot regeneration and acclimatisation of Brassica oleracea subsp. italica cv. Green Marvel |
| title | In vitro adventitious shoot regeneration and acclimatisation of Brassica oleracea subsp. italica cv. Green Marvel |
| title_full | In vitro adventitious shoot regeneration and acclimatisation of Brassica oleracea subsp. italica cv. Green Marvel |
| title_fullStr | In vitro adventitious shoot regeneration and acclimatisation of Brassica oleracea subsp. italica cv. Green Marvel |
| title_full_unstemmed | In vitro adventitious shoot regeneration and acclimatisation of Brassica oleracea subsp. italica cv. Green Marvel |
| title_short | In vitro adventitious shoot regeneration and acclimatisation of Brassica oleracea subsp. italica cv. Green Marvel |
| title_sort | in vitro adventitious shoot regeneration and acclimatisation of brassica oleracea subsp italica cv green marvel |
| url | http://psasir.upm.edu.my/id/eprint/23775/1/23775.pdf |
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