Detection and identification of Lactobacillus bacteria found in the honey stomach of the giant honeybee Apis dorsata.
This is the first assay that describes the isolation and identification of strains and species of Lactobacillus from the honey stomach of the Asiatic giant honeybee, Apis dorsata. Samples of honeybees were collected from A. dorsata colonies in different bee trees, and Lactobacillus was isolated from...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English |
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Springer Verlag
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/23846/1/Detection%20and%20identification%20of%20Lactobacillus%20bacteria%20found%20in%20the%20honey%20stomach%20of%20the%20giant%20honeybee%20Apis%20dorsata.pdf |
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| author | Tajabadi, Naser Mardan, Makhdzir Abd. Manap, Mohd. Yazid Mustafa, Shuhaimi Meimandipour, Amir Nateghi, Leila |
| author_facet | Tajabadi, Naser Mardan, Makhdzir Abd. Manap, Mohd. Yazid Mustafa, Shuhaimi Meimandipour, Amir Nateghi, Leila |
| author_sort | Tajabadi, Naser |
| collection | UPM |
| description | This is the first assay that describes the isolation and identification of strains and species of Lactobacillus from the honey stomach of the Asiatic giant honeybee, Apis dorsata. Samples of honeybees were collected from A. dorsata colonies in different bee trees, and Lactobacillus was isolated from honey stomachs using selective media. The isolates were Gram-stained and tested for catalase reaction. The 16S rRNA genes from extracted DNA of bacterial colonies were amplified with polymerase chain reaction using lactobacilli genus primers (27F and 1492R). All bacterial 16S rRNA genes were sequenced and deposited in GenBank. The 34 isolated strains yielded three distinct rRNA sequences of 15 different strains. Lactobacillus sequences isolated from the bees' honey stomachs were comprised of Lactobacillus kunkeei related-sequences (56%) with other abundant sequences being related to other Lactobacillus sp. (38%) and Lactobacillus vermiform (6%). These strains can be good candidates for potential application as probiotics in honeybees and also as natural food preservatives, which, in turn, may be useful in the food industry. |
| first_indexed | 2024-03-06T07:58:06Z |
| format | Article |
| id | upm.eprints-23846 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:58:06Z |
| publishDate | 2011 |
| publisher | Springer Verlag |
| record_format | dspace |
| spelling | upm.eprints-238462016-01-12T03:08:48Z http://psasir.upm.edu.my/id/eprint/23846/ Detection and identification of Lactobacillus bacteria found in the honey stomach of the giant honeybee Apis dorsata. Tajabadi, Naser Mardan, Makhdzir Abd. Manap, Mohd. Yazid Mustafa, Shuhaimi Meimandipour, Amir Nateghi, Leila This is the first assay that describes the isolation and identification of strains and species of Lactobacillus from the honey stomach of the Asiatic giant honeybee, Apis dorsata. Samples of honeybees were collected from A. dorsata colonies in different bee trees, and Lactobacillus was isolated from honey stomachs using selective media. The isolates were Gram-stained and tested for catalase reaction. The 16S rRNA genes from extracted DNA of bacterial colonies were amplified with polymerase chain reaction using lactobacilli genus primers (27F and 1492R). All bacterial 16S rRNA genes were sequenced and deposited in GenBank. The 34 isolated strains yielded three distinct rRNA sequences of 15 different strains. Lactobacillus sequences isolated from the bees' honey stomachs were comprised of Lactobacillus kunkeei related-sequences (56%) with other abundant sequences being related to other Lactobacillus sp. (38%) and Lactobacillus vermiform (6%). These strains can be good candidates for potential application as probiotics in honeybees and also as natural food preservatives, which, in turn, may be useful in the food industry. Springer Verlag 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/23846/1/Detection%20and%20identification%20of%20Lactobacillus%20bacteria%20found%20in%20the%20honey%20stomach%20of%20the%20giant%20honeybee%20Apis%20dorsata.pdf Tajabadi, Naser and Mardan, Makhdzir and Abd. Manap, Mohd. Yazid and Mustafa, Shuhaimi and Meimandipour, Amir and Nateghi, Leila (2011) Detection and identification of Lactobacillus bacteria found in the honey stomach of the giant honeybee Apis dorsata. Apidologie, 42 (5). pp. 642-649. ISSN 0044-8435; ESSN:1297-9678 http://www.link.springer.com/ 10.1007/s13592-011-0069-x English |
| spellingShingle | Tajabadi, Naser Mardan, Makhdzir Abd. Manap, Mohd. Yazid Mustafa, Shuhaimi Meimandipour, Amir Nateghi, Leila Detection and identification of Lactobacillus bacteria found in the honey stomach of the giant honeybee Apis dorsata. |
| title | Detection and identification of Lactobacillus bacteria found in the honey stomach of the giant honeybee Apis dorsata. |
| title_full | Detection and identification of Lactobacillus bacteria found in the honey stomach of the giant honeybee Apis dorsata. |
| title_fullStr | Detection and identification of Lactobacillus bacteria found in the honey stomach of the giant honeybee Apis dorsata. |
| title_full_unstemmed | Detection and identification of Lactobacillus bacteria found in the honey stomach of the giant honeybee Apis dorsata. |
| title_short | Detection and identification of Lactobacillus bacteria found in the honey stomach of the giant honeybee Apis dorsata. |
| title_sort | detection and identification of lactobacillus bacteria found in the honey stomach of the giant honeybee apis dorsata |
| url | http://psasir.upm.edu.my/id/eprint/23846/1/Detection%20and%20identification%20of%20Lactobacillus%20bacteria%20found%20in%20the%20honey%20stomach%20of%20the%20giant%20honeybee%20Apis%20dorsata.pdf |
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