A curcumin derivative, 2,6-bis(2,5-dimethoxybenzylidene)-cyclohexanone (BDMC33) attenuates prostaglandin E2 synthesis via selective suppression of cyclooxygenase-2 in IFN-γ/LPS-stimulated macrophages..
Our preliminary screening had shown that the curcumin derivative [2,6-bis(2,5- dimethoxybenzylidene)cyclohexanone] or BDMC33 exhibited improved anti-inflammatory activity by inhibiting nitric oxide synthesis in activated macrophage cells. In this study, we further investigated the anti-inflammatory...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English |
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MDPI
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/24101/1/A%20curcumin%20derivative.pdf |
| _version_ | 1825925502195466240 |
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| author | Lee, Ka Heng Abas, Faridah Mohamed Alitheen, Noorjahan Banu Shaari, Khozirah Lajis, Md. Nordin Ahmad, Syahida |
| author_facet | Lee, Ka Heng Abas, Faridah Mohamed Alitheen, Noorjahan Banu Shaari, Khozirah Lajis, Md. Nordin Ahmad, Syahida |
| author_sort | Lee, Ka Heng |
| collection | UPM |
| description | Our preliminary screening had shown that the curcumin derivative [2,6-bis(2,5- dimethoxybenzylidene)cyclohexanone] or BDMC33 exhibited improved anti-inflammatory activity by inhibiting nitric oxide synthesis in activated macrophage cells. In this study, we further investigated the anti-inflammatory properties of BDMC33 on PGE 2 synthesis and cyclooxygenase (COX) expression in IFN-γ/LPS-stimulated macrophages. We found that BDMC33 significantly inhibited PGE 2 synthesis in a concentration-dependent manner albeit at a low inhibition level with an IC 50 value of 47.33 ± 1.00 μM. Interestingly, the PGE2 inhibitory activity of BDMC33 is not attributed to inhibition of the COX enzyme activities, but rather BDMC33 selectively down-regulated the expression of COX-2. In addition, BDMC33 modulates the COX expression by sustaining the constitutively COX-1 expression in IFN-γ/LPS-treated macrophage cells. Collectively, the experimental data suggest an immunodulatory action of BDMC33 on PGE 2 synthesis and COX expression, making it a possible treatment for inflammatory disorders with minimal gastrointestinal related side effects. |
| first_indexed | 2024-03-06T07:58:51Z |
| format | Article |
| id | upm.eprints-24101 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:58:51Z |
| publishDate | 2011 |
| publisher | MDPI |
| record_format | dspace |
| spelling | upm.eprints-241012015-10-05T02:55:52Z http://psasir.upm.edu.my/id/eprint/24101/ A curcumin derivative, 2,6-bis(2,5-dimethoxybenzylidene)-cyclohexanone (BDMC33) attenuates prostaglandin E2 synthesis via selective suppression of cyclooxygenase-2 in IFN-γ/LPS-stimulated macrophages.. Lee, Ka Heng Abas, Faridah Mohamed Alitheen, Noorjahan Banu Shaari, Khozirah Lajis, Md. Nordin Ahmad, Syahida Our preliminary screening had shown that the curcumin derivative [2,6-bis(2,5- dimethoxybenzylidene)cyclohexanone] or BDMC33 exhibited improved anti-inflammatory activity by inhibiting nitric oxide synthesis in activated macrophage cells. In this study, we further investigated the anti-inflammatory properties of BDMC33 on PGE 2 synthesis and cyclooxygenase (COX) expression in IFN-γ/LPS-stimulated macrophages. We found that BDMC33 significantly inhibited PGE 2 synthesis in a concentration-dependent manner albeit at a low inhibition level with an IC 50 value of 47.33 ± 1.00 μM. Interestingly, the PGE2 inhibitory activity of BDMC33 is not attributed to inhibition of the COX enzyme activities, but rather BDMC33 selectively down-regulated the expression of COX-2. In addition, BDMC33 modulates the COX expression by sustaining the constitutively COX-1 expression in IFN-γ/LPS-treated macrophage cells. Collectively, the experimental data suggest an immunodulatory action of BDMC33 on PGE 2 synthesis and COX expression, making it a possible treatment for inflammatory disorders with minimal gastrointestinal related side effects. MDPI 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24101/1/A%20curcumin%20derivative.pdf Lee, Ka Heng and Abas, Faridah and Mohamed Alitheen, Noorjahan Banu and Shaari, Khozirah and Lajis, Md. Nordin and Ahmad, Syahida (2011) A curcumin derivative, 2,6-bis(2,5-dimethoxybenzylidene)-cyclohexanone (BDMC33) attenuates prostaglandin E2 synthesis via selective suppression of cyclooxygenase-2 in IFN-γ/LPS-stimulated macrophages.. Molecules, 16 (11). pp. 9728-9738. ISSN 1431-5157; ESSN: 1420-3049 10.3390/molecules16119728 English |
| spellingShingle | Lee, Ka Heng Abas, Faridah Mohamed Alitheen, Noorjahan Banu Shaari, Khozirah Lajis, Md. Nordin Ahmad, Syahida A curcumin derivative, 2,6-bis(2,5-dimethoxybenzylidene)-cyclohexanone (BDMC33) attenuates prostaglandin E2 synthesis via selective suppression of cyclooxygenase-2 in IFN-γ/LPS-stimulated macrophages.. |
| title | A curcumin derivative, 2,6-bis(2,5-dimethoxybenzylidene)-cyclohexanone (BDMC33) attenuates prostaglandin E2 synthesis via selective suppression of cyclooxygenase-2 in IFN-γ/LPS-stimulated macrophages.. |
| title_full | A curcumin derivative, 2,6-bis(2,5-dimethoxybenzylidene)-cyclohexanone (BDMC33) attenuates prostaglandin E2 synthesis via selective suppression of cyclooxygenase-2 in IFN-γ/LPS-stimulated macrophages.. |
| title_fullStr | A curcumin derivative, 2,6-bis(2,5-dimethoxybenzylidene)-cyclohexanone (BDMC33) attenuates prostaglandin E2 synthesis via selective suppression of cyclooxygenase-2 in IFN-γ/LPS-stimulated macrophages.. |
| title_full_unstemmed | A curcumin derivative, 2,6-bis(2,5-dimethoxybenzylidene)-cyclohexanone (BDMC33) attenuates prostaglandin E2 synthesis via selective suppression of cyclooxygenase-2 in IFN-γ/LPS-stimulated macrophages.. |
| title_short | A curcumin derivative, 2,6-bis(2,5-dimethoxybenzylidene)-cyclohexanone (BDMC33) attenuates prostaglandin E2 synthesis via selective suppression of cyclooxygenase-2 in IFN-γ/LPS-stimulated macrophages.. |
| title_sort | curcumin derivative 2 6 bis 2 5 dimethoxybenzylidene cyclohexanone bdmc33 attenuates prostaglandin e2 synthesis via selective suppression of cyclooxygenase 2 in ifn γ lps stimulated macrophages |
| url | http://psasir.upm.edu.my/id/eprint/24101/1/A%20curcumin%20derivative.pdf |
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