Efficient in vitro regeneration of Zingiber zerumbet Smith (a valuable medicinal plant) plantlets from rhizome bud explants
This study was conducted to develop an efficient protocol for mass propagation ofZingiber zerumbet Smith. Explants from rhizome buds were cultured on Murashige and Skoog (MS) medium supplemented with 6-Benzylaminopurine (BAP) alone (0 to 5 mg/l) or a combination of BAP (0 to 5 mg/l) and indole 3-ace...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Academic Journals
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/24252/1/24252.pdf |
| _version_ | 1825925533789061120 |
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| author | Qamaruz Zaman, Faridah Abdelmageed, Adil Hassan Ahmed Abd Aziz, Julia R., Nor Hafizah |
| author_facet | Qamaruz Zaman, Faridah Abdelmageed, Adil Hassan Ahmed Abd Aziz, Julia R., Nor Hafizah |
| author_sort | Qamaruz Zaman, Faridah |
| collection | UPM |
| description | This study was conducted to develop an efficient protocol for mass propagation ofZingiber zerumbet Smith. Explants from rhizome buds were cultured on Murashige and Skoog (MS) medium supplemented with 6-Benzylaminopurine (BAP) alone (0 to 5 mg/l) or a combination of BAP (0 to 5 mg/l) and indole 3-acetic acid (IAA) (0 to 2 mg/l). MS medium supplemented with a combination of 5.0 mg/l BAP and 2.0 mg/l IAA or 3.0 mg/l BAP and 0.5 mg/l IAA produced the highest mean number of shoots (5.6) per explant as compared to other concentrations. The best shoots length (9.44 cm) was obtained on the medium containing 1.0 mg/l of BAP and 2.0 mg/l IAA. Thus, combined effects of BAP and IAA improved significantly the shoot growth and proliferation. MS medium supplemented with a combination of 5.0 mg/l BAP and 2 mg/l IAA gave the highest number of roots (17). However, longest roots per explant were obtained with 1.0 mg/l BAP alone. The proliferated shoots were green and healthy in appearance. Finally, healthy and complete plants with well developed roots were hardened, acclimatized and planted in the field successfully with a survival rate of 80%. |
| first_indexed | 2024-03-06T07:59:18Z |
| format | Article |
| id | upm.eprints-24252 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:59:18Z |
| publishDate | 2011 |
| publisher | Academic Journals |
| record_format | dspace |
| spelling | upm.eprints-242522016-06-30T02:57:18Z http://psasir.upm.edu.my/id/eprint/24252/ Efficient in vitro regeneration of Zingiber zerumbet Smith (a valuable medicinal plant) plantlets from rhizome bud explants Qamaruz Zaman, Faridah Abdelmageed, Adil Hassan Ahmed Abd Aziz, Julia R., Nor Hafizah This study was conducted to develop an efficient protocol for mass propagation ofZingiber zerumbet Smith. Explants from rhizome buds were cultured on Murashige and Skoog (MS) medium supplemented with 6-Benzylaminopurine (BAP) alone (0 to 5 mg/l) or a combination of BAP (0 to 5 mg/l) and indole 3-acetic acid (IAA) (0 to 2 mg/l). MS medium supplemented with a combination of 5.0 mg/l BAP and 2.0 mg/l IAA or 3.0 mg/l BAP and 0.5 mg/l IAA produced the highest mean number of shoots (5.6) per explant as compared to other concentrations. The best shoots length (9.44 cm) was obtained on the medium containing 1.0 mg/l of BAP and 2.0 mg/l IAA. Thus, combined effects of BAP and IAA improved significantly the shoot growth and proliferation. MS medium supplemented with a combination of 5.0 mg/l BAP and 2 mg/l IAA gave the highest number of roots (17). However, longest roots per explant were obtained with 1.0 mg/l BAP alone. The proliferated shoots were green and healthy in appearance. Finally, healthy and complete plants with well developed roots were hardened, acclimatized and planted in the field successfully with a survival rate of 80%. Academic Journals 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24252/1/24252.pdf Qamaruz Zaman, Faridah and Abdelmageed, Adil Hassan Ahmed and Abd Aziz, Julia and R., Nor Hafizah (2011) Efficient in vitro regeneration of Zingiber zerumbet Smith (a valuable medicinal plant) plantlets from rhizome bud explants. African Journal of Biotechnology, 10 (46). art. no. B01A6C226273. pp. 9303-9308. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/B01A6C226273 10.5897/AJB11.1182 |
| spellingShingle | Qamaruz Zaman, Faridah Abdelmageed, Adil Hassan Ahmed Abd Aziz, Julia R., Nor Hafizah Efficient in vitro regeneration of Zingiber zerumbet Smith (a valuable medicinal plant) plantlets from rhizome bud explants |
| title | Efficient in vitro regeneration of Zingiber zerumbet Smith (a valuable medicinal plant) plantlets from rhizome bud explants |
| title_full | Efficient in vitro regeneration of Zingiber zerumbet Smith (a valuable medicinal plant) plantlets from rhizome bud explants |
| title_fullStr | Efficient in vitro regeneration of Zingiber zerumbet Smith (a valuable medicinal plant) plantlets from rhizome bud explants |
| title_full_unstemmed | Efficient in vitro regeneration of Zingiber zerumbet Smith (a valuable medicinal plant) plantlets from rhizome bud explants |
| title_short | Efficient in vitro regeneration of Zingiber zerumbet Smith (a valuable medicinal plant) plantlets from rhizome bud explants |
| title_sort | efficient in vitro regeneration of zingiber zerumbet smith a valuable medicinal plant plantlets from rhizome bud explants |
| url | http://psasir.upm.edu.my/id/eprint/24252/1/24252.pdf |
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