Comparison of DNA polymorphism of bovine pituitary-specific transcription Factor and Leptin Gene Between Iranian Bos indicus and Bos taurus Cattle Using PCR-RFLP.

Variations at DNA level contribute to the genetic characterization of livestock populations and this may help to identify possible hybridization events as well as past evolutionary trends. The leptin and Pit-1 are attractive candidate genes for production and reproduction traits in cattle. A total o...

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Main Authors: Nahavandi, Reza, Asadzadeh, Nader, Farjam, Soleimani, Nurul Amin, S. M., Hafezamini, P., Javanmard, A.
Format: Article
Language:English
English
Published: Medwell Online 2010
Online Access:http://psasir.upm.edu.my/id/eprint/24253/1/Comparison%20of%20DNA%20polymorphism%20of%20bovine%20pituitary.pdf
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author Nahavandi, Reza
Asadzadeh, Nader
Farjam, Soleimani
Nurul Amin, S. M.
Hafezamini, P.
Javanmard, A.
author_facet Nahavandi, Reza
Asadzadeh, Nader
Farjam, Soleimani
Nurul Amin, S. M.
Hafezamini, P.
Javanmard, A.
author_sort Nahavandi, Reza
collection UPM
description Variations at DNA level contribute to the genetic characterization of livestock populations and this may help to identify possible hybridization events as well as past evolutionary trends. The leptin and Pit-1 are attractive candidate genes for production and reproduction traits in cattle. A total of 247 animals from four breeds from two species of Iranian cattle populations in include Bos taurus (Sarabi, Golpayegani) and Bos indicus (Sistani, Taleshi) were genotyped for the Pit-1 Hinfl and leptin Sau3AI polymorphisms by the Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP). The genotype and gene frequencies for each group were determined and shown to be quite variable among the breeds. The allele B for the leptin gene and allele A for the Pit-1 gene were investigated high frequency in Bos indicus. Candidate gene approach may be a useful method to measure of genetic distance for cross breeding program between taurin and indicine cattle.
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spelling upm.eprints-242532015-12-16T07:49:16Z http://psasir.upm.edu.my/id/eprint/24253/ Comparison of DNA polymorphism of bovine pituitary-specific transcription Factor and Leptin Gene Between Iranian Bos indicus and Bos taurus Cattle Using PCR-RFLP. Nahavandi, Reza Asadzadeh, Nader Farjam, Soleimani Nurul Amin, S. M. Hafezamini, P. Javanmard, A. Variations at DNA level contribute to the genetic characterization of livestock populations and this may help to identify possible hybridization events as well as past evolutionary trends. The leptin and Pit-1 are attractive candidate genes for production and reproduction traits in cattle. A total of 247 animals from four breeds from two species of Iranian cattle populations in include Bos taurus (Sarabi, Golpayegani) and Bos indicus (Sistani, Taleshi) were genotyped for the Pit-1 Hinfl and leptin Sau3AI polymorphisms by the Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP). The genotype and gene frequencies for each group were determined and shown to be quite variable among the breeds. The allele B for the leptin gene and allele A for the Pit-1 gene were investigated high frequency in Bos indicus. Candidate gene approach may be a useful method to measure of genetic distance for cross breeding program between taurin and indicine cattle. Medwell Online 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24253/1/Comparison%20of%20DNA%20polymorphism%20of%20bovine%20pituitary.pdf Nahavandi, Reza and Asadzadeh, Nader and Farjam, Soleimani and Nurul Amin, S. M. and Hafezamini, P. and Javanmard, A. (2010) Comparison of DNA polymorphism of bovine pituitary-specific transcription Factor and Leptin Gene Between Iranian Bos indicus and Bos taurus Cattle Using PCR-RFLP. Journal of Animal and Veterinary Advances, 9 (11). pp. 1660-1663. ISSN 1680-5593 http://www.medwelljournals.com/ 10.3923/javaa.2010.1660.1663 English
spellingShingle Nahavandi, Reza
Asadzadeh, Nader
Farjam, Soleimani
Nurul Amin, S. M.
Hafezamini, P.
Javanmard, A.
Comparison of DNA polymorphism of bovine pituitary-specific transcription Factor and Leptin Gene Between Iranian Bos indicus and Bos taurus Cattle Using PCR-RFLP.
title Comparison of DNA polymorphism of bovine pituitary-specific transcription Factor and Leptin Gene Between Iranian Bos indicus and Bos taurus Cattle Using PCR-RFLP.
title_full Comparison of DNA polymorphism of bovine pituitary-specific transcription Factor and Leptin Gene Between Iranian Bos indicus and Bos taurus Cattle Using PCR-RFLP.
title_fullStr Comparison of DNA polymorphism of bovine pituitary-specific transcription Factor and Leptin Gene Between Iranian Bos indicus and Bos taurus Cattle Using PCR-RFLP.
title_full_unstemmed Comparison of DNA polymorphism of bovine pituitary-specific transcription Factor and Leptin Gene Between Iranian Bos indicus and Bos taurus Cattle Using PCR-RFLP.
title_short Comparison of DNA polymorphism of bovine pituitary-specific transcription Factor and Leptin Gene Between Iranian Bos indicus and Bos taurus Cattle Using PCR-RFLP.
title_sort comparison of dna polymorphism of bovine pituitary specific transcription factor and leptin gene between iranian bos indicus and bos taurus cattle using pcr rflp
url http://psasir.upm.edu.my/id/eprint/24253/1/Comparison%20of%20DNA%20polymorphism%20of%20bovine%20pituitary.pdf
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