Assessing estrogenic activity of Nigella sativa in ovariectomized rats using vaginal cornification assay.
The aim of this study was to determine the estrogenic activity of Nigella sativa (NS) by vaginal cornification assay using an ovariectomized rat model. Forty ovariectomized Sprague Dawley rats, weighting 250 to 350 g were used in the study. N. sativa powders were administered to ovariectomized...
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| Format: | Article |
| Language: | English English |
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Academic Journals
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/24424/1/Assessing%20estrogenic%20activity%20of%20Nigella%20sativa%20in%20ovariectomized%20rats%20using%20vaginal%20cornification%20assay.pdf |
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| author | Parhizkar, Saadat Abdul Latiff, Latiffah Abdul Rahman, Sabariah Dollah, Mohammad Aziz Parichehr, Hanachi |
| author_facet | Parhizkar, Saadat Abdul Latiff, Latiffah Abdul Rahman, Sabariah Dollah, Mohammad Aziz Parichehr, Hanachi |
| author_sort | Parhizkar, Saadat |
| collection | UPM |
| description | The aim of this study was to determine the estrogenic activity of Nigella sativa (NS) by vaginal
cornification assay using an ovariectomized rat model. Forty ovariectomized Sprague Dawley rats,
weighting 250 to 350 g were used in the study. N. sativa powders were administered to ovariectomized
rats for 21 consecutive days at dosages of 300, 600 and 1200 mg/kg respectively, and were compared
with each of daily treatment with 0.2 mg/kg conjugated Equine estrogen as positive control or distilled
water as negative control. Vaginal smears were taken to observe the estrogenic effect on vaginal
epithelium of rats. The vaginal smear showed an atrophic pattern at baseline. The occurrence of vaginal
cornification after N. sativa supplementation indicated estrogenic activity of N. sativa, but this effect
was not as much as CEE. The most influence of N. sativa in vaginal cornification was observed in low
dose NS that this result was in agreement with serum Estradiol level of this group. The cornified cells
percentage significantly differed from control group (P<0.05). These data suggest that N. sativa
possesses estrogenic function in the ovariectomized rat model which can be helpful in managing
menopausal symptoms as an alternative for Hormone Replacement Therapy.
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| first_indexed | 2024-03-06T07:59:49Z |
| format | Article |
| id | upm.eprints-24424 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:59:49Z |
| publishDate | 2011 |
| publisher | Academic Journals |
| record_format | dspace |
| spelling | upm.eprints-244242015-10-28T07:23:10Z http://psasir.upm.edu.my/id/eprint/24424/ Assessing estrogenic activity of Nigella sativa in ovariectomized rats using vaginal cornification assay. Parhizkar, Saadat Abdul Latiff, Latiffah Abdul Rahman, Sabariah Dollah, Mohammad Aziz Parichehr, Hanachi The aim of this study was to determine the estrogenic activity of Nigella sativa (NS) by vaginal cornification assay using an ovariectomized rat model. Forty ovariectomized Sprague Dawley rats, weighting 250 to 350 g were used in the study. N. sativa powders were administered to ovariectomized rats for 21 consecutive days at dosages of 300, 600 and 1200 mg/kg respectively, and were compared with each of daily treatment with 0.2 mg/kg conjugated Equine estrogen as positive control or distilled water as negative control. Vaginal smears were taken to observe the estrogenic effect on vaginal epithelium of rats. The vaginal smear showed an atrophic pattern at baseline. The occurrence of vaginal cornification after N. sativa supplementation indicated estrogenic activity of N. sativa, but this effect was not as much as CEE. The most influence of N. sativa in vaginal cornification was observed in low dose NS that this result was in agreement with serum Estradiol level of this group. The cornified cells percentage significantly differed from control group (P<0.05). These data suggest that N. sativa possesses estrogenic function in the ovariectomized rat model which can be helpful in managing menopausal symptoms as an alternative for Hormone Replacement Therapy. Academic Journals 2011-02 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24424/1/Assessing%20estrogenic%20activity%20of%20Nigella%20sativa%20in%20ovariectomized%20rats%20using%20vaginal%20cornification%20assay.pdf Parhizkar, Saadat and Abdul Latiff, Latiffah and Abdul Rahman, Sabariah and Dollah, Mohammad Aziz and Parichehr, Hanachi (2011) Assessing estrogenic activity of Nigella sativa in ovariectomized rats using vaginal cornification assay. African Journal of Pharmacy and Pharmacology, 5 (2 ). pp. 137-142. ISSN 1996-0816 http://www.academicjournals.org/ajpp English |
| spellingShingle | Parhizkar, Saadat Abdul Latiff, Latiffah Abdul Rahman, Sabariah Dollah, Mohammad Aziz Parichehr, Hanachi Assessing estrogenic activity of Nigella sativa in ovariectomized rats using vaginal cornification assay. |
| title | Assessing estrogenic activity of Nigella sativa in ovariectomized rats using vaginal cornification assay. |
| title_full | Assessing estrogenic activity of Nigella sativa in ovariectomized rats using vaginal cornification assay. |
| title_fullStr | Assessing estrogenic activity of Nigella sativa in ovariectomized rats using vaginal cornification assay. |
| title_full_unstemmed | Assessing estrogenic activity of Nigella sativa in ovariectomized rats using vaginal cornification assay. |
| title_short | Assessing estrogenic activity of Nigella sativa in ovariectomized rats using vaginal cornification assay. |
| title_sort | assessing estrogenic activity of nigella sativa in ovariectomized rats using vaginal cornification assay |
| url | http://psasir.upm.edu.my/id/eprint/24424/1/Assessing%20estrogenic%20activity%20of%20Nigella%20sativa%20in%20ovariectomized%20rats%20using%20vaginal%20cornification%20assay.pdf |
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