Effects of hydrolysis conditions on recovery of antioxidants from methanolic extracts of Nigella Sativa seeds.
Nigella sativa (NS) has health promoting beneficial properties including antioxidant activity. In this study, the impact of acid and alkaline hydrolysis conditions on phenolic (TPC), flavonoid (TFC) contents and antioxidant activity of methanolic extracts from N. sativa (NS) seeds powder is evaluate...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English English |
Published: |
Academic Journals
2011
|
Online Access: | http://psasir.upm.edu.my/id/eprint/24487/1/Effects%20of%20hydrolysis%20conditions%20on%20recovery%20of%20antioxidants%20from%20methanolic%20extracts%20of%20Nigella%20Sativa%20seeds.pdf |
_version_ | 1796970569820798976 |
---|---|
author | Tubesha, Zaki Iqbal, Shahid Ismail, Maznah |
author_facet | Tubesha, Zaki Iqbal, Shahid Ismail, Maznah |
author_sort | Tubesha, Zaki |
collection | UPM |
description | Nigella sativa (NS) has health promoting beneficial properties including antioxidant activity. In this study, the impact of acid and alkaline hydrolysis conditions on phenolic (TPC), flavonoid (TFC) contents and antioxidant activity of methanolic extracts from N. sativa (NS) seeds powder is evaluated. Total phenolic and flavonoid contents were evaluated according to Folin-Ciocalteu procedure and aluminium chloride colorimetric assays, respectively. The TPC extracts varied from 67 to 73 mg/100 g NS powder, expressed as Gallic acid equivalents (GAE), while TFC concentrations varied from 96 to 113 mg rutin equivalents (RE)/100 g NS powder. Positive correlations were found between TPC and TFC in NS hydrolyzates and their1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (r2 = 0.975 and 0.978, respectively). The alkaline hydrolyzed extract (BHE) showed the highest TPC and TFC (730 µg GAE and1130 µg RE/g NS powder, respectively) as well as the highest antioxidant activity (DPPH• =167±0.133 mg TEAC /100 g dry NS, ABTS•+=112 ± 0.023 mg TEAC /100 g dry NS and FRAP=28 ± 0.159 mg GAE/100 g dry NS) compared to acid hydrolyzed (AHE) and non-hydrolyzed extracts (NHE). Non-hydrolyzed extracts showed the lowest TPC and TFC content (670 µg GAE and 960 µg RE/g NS powder, respectively) through the assays (p < 0.05). Findings of the study reveal that hydrolysis has profound effects on recovery of antioxidants from NS extracts. Overall, BHE has the highest antioxidant activity compared to AHE or NHE. |
first_indexed | 2024-03-06T08:00:00Z |
format | Article |
id | upm.eprints-24487 |
institution | Universiti Putra Malaysia |
language | English English |
last_indexed | 2024-03-06T08:00:00Z |
publishDate | 2011 |
publisher | Academic Journals |
record_format | dspace |
spelling | upm.eprints-244872016-01-12T01:49:32Z http://psasir.upm.edu.my/id/eprint/24487/ Effects of hydrolysis conditions on recovery of antioxidants from methanolic extracts of Nigella Sativa seeds. Tubesha, Zaki Iqbal, Shahid Ismail, Maznah Nigella sativa (NS) has health promoting beneficial properties including antioxidant activity. In this study, the impact of acid and alkaline hydrolysis conditions on phenolic (TPC), flavonoid (TFC) contents and antioxidant activity of methanolic extracts from N. sativa (NS) seeds powder is evaluated. Total phenolic and flavonoid contents were evaluated according to Folin-Ciocalteu procedure and aluminium chloride colorimetric assays, respectively. The TPC extracts varied from 67 to 73 mg/100 g NS powder, expressed as Gallic acid equivalents (GAE), while TFC concentrations varied from 96 to 113 mg rutin equivalents (RE)/100 g NS powder. Positive correlations were found between TPC and TFC in NS hydrolyzates and their1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (r2 = 0.975 and 0.978, respectively). The alkaline hydrolyzed extract (BHE) showed the highest TPC and TFC (730 µg GAE and1130 µg RE/g NS powder, respectively) as well as the highest antioxidant activity (DPPH• =167±0.133 mg TEAC /100 g dry NS, ABTS•+=112 ± 0.023 mg TEAC /100 g dry NS and FRAP=28 ± 0.159 mg GAE/100 g dry NS) compared to acid hydrolyzed (AHE) and non-hydrolyzed extracts (NHE). Non-hydrolyzed extracts showed the lowest TPC and TFC content (670 µg GAE and 960 µg RE/g NS powder, respectively) through the assays (p < 0.05). Findings of the study reveal that hydrolysis has profound effects on recovery of antioxidants from NS extracts. Overall, BHE has the highest antioxidant activity compared to AHE or NHE. Academic Journals 2011-10-16 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24487/1/Effects%20of%20hydrolysis%20conditions%20on%20recovery%20of%20antioxidants%20from%20methanolic%20extracts%20of%20Nigella%20Sativa%20seeds.pdf Tubesha, Zaki and Iqbal, Shahid and Ismail, Maznah (2011) Effects of hydrolysis conditions on recovery of antioxidants from methanolic extracts of Nigella Sativa seeds. Journal of Medicinal Plants Research, 5 (22). pp. 5393-5399. ISSN 1996-0875 http://www.academicjournals.org/journal/JMPR English |
spellingShingle | Tubesha, Zaki Iqbal, Shahid Ismail, Maznah Effects of hydrolysis conditions on recovery of antioxidants from methanolic extracts of Nigella Sativa seeds. |
title | Effects of hydrolysis conditions on recovery of antioxidants from methanolic extracts of Nigella Sativa seeds. |
title_full | Effects of hydrolysis conditions on recovery of antioxidants from methanolic extracts of Nigella Sativa seeds. |
title_fullStr | Effects of hydrolysis conditions on recovery of antioxidants from methanolic extracts of Nigella Sativa seeds. |
title_full_unstemmed | Effects of hydrolysis conditions on recovery of antioxidants from methanolic extracts of Nigella Sativa seeds. |
title_short | Effects of hydrolysis conditions on recovery of antioxidants from methanolic extracts of Nigella Sativa seeds. |
title_sort | effects of hydrolysis conditions on recovery of antioxidants from methanolic extracts of nigella sativa seeds |
url | http://psasir.upm.edu.my/id/eprint/24487/1/Effects%20of%20hydrolysis%20conditions%20on%20recovery%20of%20antioxidants%20from%20methanolic%20extracts%20of%20Nigella%20Sativa%20seeds.pdf |
work_keys_str_mv | AT tubeshazaki effectsofhydrolysisconditionsonrecoveryofantioxidantsfrommethanolicextractsofnigellasativaseeds AT iqbalshahid effectsofhydrolysisconditionsonrecoveryofantioxidantsfrommethanolicextractsofnigellasativaseeds AT ismailmaznah effectsofhydrolysisconditionsonrecoveryofantioxidantsfrommethanolicextractsofnigellasativaseeds |