Qualitative flow cytometric analysis of Malaysian myelodysplastic syndromes (MDS) patients.
Myelodysplastic syndromes (MDS) are a group of haematological malignancies categorized by ineffective hematopoiesis that result in dysplasia. Although morphological diagnosis is a traditional and standard technique that is used for the diagnosis of MDS, the heterogeneous blood and bone marrow charac...
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| Format: | Article |
| Language: | English English |
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Malaysian Medical Association
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/24507/1/Qualitative%20flow%20cytometric%20analysis%20of%20Malaysian%20myelodysplastic%20syndromes.pdf |
| _version_ | 1796970573851525120 |
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| author | Broojerdi, Mohadese Hashem Ramasamy, Rajesh Md Noor, Sabariah Seman, Zainina |
| author_facet | Broojerdi, Mohadese Hashem Ramasamy, Rajesh Md Noor, Sabariah Seman, Zainina |
| author_sort | Broojerdi, Mohadese Hashem |
| collection | UPM |
| description | Myelodysplastic syndromes (MDS) are a group of haematological malignancies categorized by ineffective hematopoiesis that result in dysplasia. Although morphological diagnosis is a traditional and standard technique that is used for the diagnosis of MDS, the heterogeneous blood and bone marrow characteristics of MDS patients can potentially obscure the right diagnosis. Thus, we have utilized flow cytometric immunophenotyping as a supportive mechanism to obtain a more accurate and faster method for detection of abnormal markers in MDS. Flow cytometry was used for analyzing bone marrow samples from newly diagnosed MDS patients to investigate the abnormal antigen expression patterns in granulocytic, monocytic, erythroid, lymphoid lineages and myeloid precursors. The results were compared with those obtained from cases that had Idiopathic Thrombocytopenic Purpura (ITP) as a control. The most common abnormality found in the granulocytic lineage was the decrease of CD10. Low expressions of CD13 were the most frequent abnormality in the monocytic lineage. The erythroid lineage was found to have low expression of CD235A+/CD71+, reduce of CD71 and decreased CD235a. In conclusion, this method is useful for confirming cases in which it is difficult to make a diagnosis by morphology. |
| first_indexed | 2024-03-06T08:00:04Z |
| format | Article |
| id | upm.eprints-24507 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T08:00:04Z |
| publishDate | 2012 |
| publisher | Malaysian Medical Association |
| record_format | dspace |
| spelling | upm.eprints-245072015-08-26T03:04:40Z http://psasir.upm.edu.my/id/eprint/24507/ Qualitative flow cytometric analysis of Malaysian myelodysplastic syndromes (MDS) patients. Broojerdi, Mohadese Hashem Ramasamy, Rajesh Md Noor, Sabariah Seman, Zainina Myelodysplastic syndromes (MDS) are a group of haematological malignancies categorized by ineffective hematopoiesis that result in dysplasia. Although morphological diagnosis is a traditional and standard technique that is used for the diagnosis of MDS, the heterogeneous blood and bone marrow characteristics of MDS patients can potentially obscure the right diagnosis. Thus, we have utilized flow cytometric immunophenotyping as a supportive mechanism to obtain a more accurate and faster method for detection of abnormal markers in MDS. Flow cytometry was used for analyzing bone marrow samples from newly diagnosed MDS patients to investigate the abnormal antigen expression patterns in granulocytic, monocytic, erythroid, lymphoid lineages and myeloid precursors. The results were compared with those obtained from cases that had Idiopathic Thrombocytopenic Purpura (ITP) as a control. The most common abnormality found in the granulocytic lineage was the decrease of CD10. Low expressions of CD13 were the most frequent abnormality in the monocytic lineage. The erythroid lineage was found to have low expression of CD235A+/CD71+, reduce of CD71 and decreased CD235a. In conclusion, this method is useful for confirming cases in which it is difficult to make a diagnosis by morphology. Malaysian Medical Association 2012 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24507/1/Qualitative%20flow%20cytometric%20analysis%20of%20Malaysian%20myelodysplastic%20syndromes.pdf Broojerdi, Mohadese Hashem and Ramasamy, Rajesh and Md Noor, Sabariah and Seman, Zainina (2012) Qualitative flow cytometric analysis of Malaysian myelodysplastic syndromes (MDS) patients. Medical Journal of Malaysia, 67 (1). pp. 77-80. ISSN 0300-5283 http://www.e-mjm.org/ English |
| spellingShingle | Broojerdi, Mohadese Hashem Ramasamy, Rajesh Md Noor, Sabariah Seman, Zainina Qualitative flow cytometric analysis of Malaysian myelodysplastic syndromes (MDS) patients. |
| title | Qualitative flow cytometric analysis of Malaysian myelodysplastic syndromes (MDS) patients. |
| title_full | Qualitative flow cytometric analysis of Malaysian myelodysplastic syndromes (MDS) patients. |
| title_fullStr | Qualitative flow cytometric analysis of Malaysian myelodysplastic syndromes (MDS) patients. |
| title_full_unstemmed | Qualitative flow cytometric analysis of Malaysian myelodysplastic syndromes (MDS) patients. |
| title_short | Qualitative flow cytometric analysis of Malaysian myelodysplastic syndromes (MDS) patients. |
| title_sort | qualitative flow cytometric analysis of malaysian myelodysplastic syndromes mds patients |
| url | http://psasir.upm.edu.my/id/eprint/24507/1/Qualitative%20flow%20cytometric%20analysis%20of%20Malaysian%20myelodysplastic%20syndromes.pdf |
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