Andrographolide induces cell cycle arrest and apoptosis in PC-3 prostate cancer cells.
Andrographolide (AGP), the major phytoconstituent isolated from Andrographis paniculata was found to exhibit growth inhibition and cytotoxicity against the hormone-independent (PC-3 and DU-145) and hormone-dependent (LNCaP) prostate cancer cell lines via the microculture tetrazolium (MTT) assay. Due...
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| Format: | Article |
| Language: | English English |
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Academic Journals
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/24539/1/Andrographolide%20induces%20cell%20cycle%20arrest%20and%20apoptosis%20in%20PC.pdf |
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| author | Hui, Chyn Wong Sagineedu, Sreenivasa Rao Lajis, Md. Nordin Loke, Seng Cheong Stanslas, Johnson |
| author_facet | Hui, Chyn Wong Sagineedu, Sreenivasa Rao Lajis, Md. Nordin Loke, Seng Cheong Stanslas, Johnson |
| author_sort | Hui, Chyn Wong |
| collection | UPM |
| description | Andrographolide (AGP), the major phytoconstituent isolated from Andrographis paniculata was found to exhibit growth inhibition and cytotoxicity against the hormone-independent (PC-3 and DU-145) and hormone-dependent (LNCaP) prostate cancer cell lines via the microculture tetrazolium (MTT) assay. Due to its greater cytotoxic potency and selectivity towards PC-3 cells, flow cytometry was used to analyze the cell cycle distribution of control and treated PC-3 cells whereas Annexin V-FITC/PI flow cytometry analysis was carried out to confirm apoptosis induced by AGP in PC-3 cells. Cell cycle and apoptotic regulatory proteins were determined by western blot analysis. AGP was found to induce G2/M cell cycle arrest which led to predominantly apoptotic mode of cell death. Mechanistically, AGP was found to downregulate CDK1 without affecting the levels of CDK4 and cyclin D1. Induction of apoptosis was associated with an increase in activation and expression of caspase 8 which then is believed to have induced cleavage of Bid into tBid. In addition, activation and enhancement of executioner caspase 9 and Bax proteins without affecting Bcl-2 protein levels were observed. |
| first_indexed | 2024-03-06T08:00:09Z |
| format | Article |
| id | upm.eprints-24539 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T08:00:09Z |
| publishDate | 2011 |
| publisher | Academic Journals |
| record_format | dspace |
| spelling | upm.eprints-245392015-09-14T01:06:35Z http://psasir.upm.edu.my/id/eprint/24539/ Andrographolide induces cell cycle arrest and apoptosis in PC-3 prostate cancer cells. Hui, Chyn Wong Sagineedu, Sreenivasa Rao Lajis, Md. Nordin Loke, Seng Cheong Stanslas, Johnson Andrographolide (AGP), the major phytoconstituent isolated from Andrographis paniculata was found to exhibit growth inhibition and cytotoxicity against the hormone-independent (PC-3 and DU-145) and hormone-dependent (LNCaP) prostate cancer cell lines via the microculture tetrazolium (MTT) assay. Due to its greater cytotoxic potency and selectivity towards PC-3 cells, flow cytometry was used to analyze the cell cycle distribution of control and treated PC-3 cells whereas Annexin V-FITC/PI flow cytometry analysis was carried out to confirm apoptosis induced by AGP in PC-3 cells. Cell cycle and apoptotic regulatory proteins were determined by western blot analysis. AGP was found to induce G2/M cell cycle arrest which led to predominantly apoptotic mode of cell death. Mechanistically, AGP was found to downregulate CDK1 without affecting the levels of CDK4 and cyclin D1. Induction of apoptosis was associated with an increase in activation and expression of caspase 8 which then is believed to have induced cleavage of Bid into tBid. In addition, activation and enhancement of executioner caspase 9 and Bax proteins without affecting Bcl-2 protein levels were observed. Academic Journals 2011-02 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24539/1/Andrographolide%20induces%20cell%20cycle%20arrest%20and%20apoptosis%20in%20PC.pdf Hui, Chyn Wong and Sagineedu, Sreenivasa Rao and Lajis, Md. Nordin and Loke, Seng Cheong and Stanslas, Johnson (2011) Andrographolide induces cell cycle arrest and apoptosis in PC-3 prostate cancer cells. African Journal of Pharmacy and Pharmacology, 5 (2). pp. 225-233. ISSN 1996-0816 http://www.academicjournals.org/journal/AJPP 10.5897/AJMR10.383 English |
| spellingShingle | Hui, Chyn Wong Sagineedu, Sreenivasa Rao Lajis, Md. Nordin Loke, Seng Cheong Stanslas, Johnson Andrographolide induces cell cycle arrest and apoptosis in PC-3 prostate cancer cells. |
| title | Andrographolide induces cell cycle arrest and apoptosis in PC-3 prostate cancer cells. |
| title_full | Andrographolide induces cell cycle arrest and apoptosis in PC-3 prostate cancer cells. |
| title_fullStr | Andrographolide induces cell cycle arrest and apoptosis in PC-3 prostate cancer cells. |
| title_full_unstemmed | Andrographolide induces cell cycle arrest and apoptosis in PC-3 prostate cancer cells. |
| title_short | Andrographolide induces cell cycle arrest and apoptosis in PC-3 prostate cancer cells. |
| title_sort | andrographolide induces cell cycle arrest and apoptosis in pc 3 prostate cancer cells |
| url | http://psasir.upm.edu.my/id/eprint/24539/1/Andrographolide%20induces%20cell%20cycle%20arrest%20and%20apoptosis%20in%20PC.pdf |
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