Two-dimensional profiling of proteins from Curculigo latifolia fruit by three different extraction protocols.
Lemba, Curculigo latifolia (family Hypoxidaceae) is an indigenous plant of Malaysia, found mainly in swampy areas of tropical Asia and Australia. This study was designed to compare the efficiency of three protocols employed for the extraction of proteins from C. latifolia fruit and to find the b...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Academic Journals
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/24543/1/Two.pdf |
| _version_ | 1796970580101038080 |
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| author | Rastegari, Elham Ahmad, Zalinah Spencer, David F. Ismail, Maznah |
| author_facet | Rastegari, Elham Ahmad, Zalinah Spencer, David F. Ismail, Maznah |
| author_sort | Rastegari, Elham |
| collection | UPM |
| description | Lemba, Curculigo latifolia (family Hypoxidaceae) is an indigenous plant of Malaysia, found mainly in
swampy areas of tropical Asia and Australia. This study was designed to compare the efficiency of
three protocols employed for the extraction of proteins from C. latifolia fruit and to find the best
compatible with proteomic analysis of fruit on the basis of profiling using two-dimensional gel
electrophoresis. Phenol, trichloroacetic acid–acetone and trichloroacetic acid–acetone/phenol-based
extraction protocols were evaluated by examining the quantitative and qualitative characteristics of the
extracted proteins. A few modifications were introduced to the phenol and the combination of phenol
and trichloroacetic acid–acetoneprotocols in order to improve the two-dimensional gel electrophoresis
analysis results. With the exception of trichloroacetic acid–acetone method, the two other protocols
were found to extract proteins efficiently and reproducibly. The protein yields from the phenol (3.5 ±
0.12 mg/g) and trichloroacetic acid–acetone/phenol-based (3.7 ± 0.11 mg/g) protocols as well as and the
two-dimensional gel electrophoresis patterns showed no appreciable differences. Since using phenol
protocol is considerably more time consuming and laborious than the trichloroacetic acid–
acetone/phenol-based protocol, therefore trichloroacetic acid–acetone/phenol-based protocol was
considered to be a superior protocol for total proteins extraction of C. latifolia fruit.
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| first_indexed | 2024-03-06T08:00:09Z |
| format | Article |
| id | upm.eprints-24543 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T08:00:09Z |
| publishDate | 2011 |
| publisher | Academic Journals |
| record_format | dspace |
| spelling | upm.eprints-245432015-11-02T01:55:33Z http://psasir.upm.edu.my/id/eprint/24543/ Two-dimensional profiling of proteins from Curculigo latifolia fruit by three different extraction protocols. Rastegari, Elham Ahmad, Zalinah Spencer, David F. Ismail, Maznah Lemba, Curculigo latifolia (family Hypoxidaceae) is an indigenous plant of Malaysia, found mainly in swampy areas of tropical Asia and Australia. This study was designed to compare the efficiency of three protocols employed for the extraction of proteins from C. latifolia fruit and to find the best compatible with proteomic analysis of fruit on the basis of profiling using two-dimensional gel electrophoresis. Phenol, trichloroacetic acid–acetone and trichloroacetic acid–acetone/phenol-based extraction protocols were evaluated by examining the quantitative and qualitative characteristics of the extracted proteins. A few modifications were introduced to the phenol and the combination of phenol and trichloroacetic acid–acetoneprotocols in order to improve the two-dimensional gel electrophoresis analysis results. With the exception of trichloroacetic acid–acetone method, the two other protocols were found to extract proteins efficiently and reproducibly. The protein yields from the phenol (3.5 ± 0.12 mg/g) and trichloroacetic acid–acetone/phenol-based (3.7 ± 0.11 mg/g) protocols as well as and the two-dimensional gel electrophoresis patterns showed no appreciable differences. Since using phenol protocol is considerably more time consuming and laborious than the trichloroacetic acid– acetone/phenol-based protocol, therefore trichloroacetic acid–acetone/phenol-based protocol was considered to be a superior protocol for total proteins extraction of C. latifolia fruit. Academic Journals 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24543/1/Two.pdf Rastegari, Elham and Ahmad, Zalinah and Spencer, David F. and Ismail, Maznah (2011) Two-dimensional profiling of proteins from Curculigo latifolia fruit by three different extraction protocols. Journal of Medicinal Plants Research, 5 (16). pp. 3719-3724. ISSN 1996-0875 http://www.academicjournals.org/journal/JMPR English |
| spellingShingle | Rastegari, Elham Ahmad, Zalinah Spencer, David F. Ismail, Maznah Two-dimensional profiling of proteins from Curculigo latifolia fruit by three different extraction protocols. |
| title | Two-dimensional profiling of proteins from Curculigo
latifolia fruit by three different extraction protocols. |
| title_full | Two-dimensional profiling of proteins from Curculigo
latifolia fruit by three different extraction protocols. |
| title_fullStr | Two-dimensional profiling of proteins from Curculigo
latifolia fruit by three different extraction protocols. |
| title_full_unstemmed | Two-dimensional profiling of proteins from Curculigo
latifolia fruit by three different extraction protocols. |
| title_short | Two-dimensional profiling of proteins from Curculigo
latifolia fruit by three different extraction protocols. |
| title_sort | two dimensional profiling of proteins from curculigo latifolia fruit by three different extraction protocols |
| url | http://psasir.upm.edu.my/id/eprint/24543/1/Two.pdf |
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