Determination of folate content in commonly consumed Malaysian foods

Currently, data concerning the content of naturally occurring dietary folate in Malaysian foods is scarce. The aim of this study was to determine the folate content of vegetables, fruits, legumes and cereals that were commonly consumed among Malaysians. The total folate content of 156 samples (51 ve...

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Main Authors: Chew, S. C., Loh, Su Peng, Khor, Geok Lin
Format: Article
Language:English
English
English
Published: Faculty of Food Science and Technology, Universiti Putra Malaysia 2012
Online Access:http://psasir.upm.edu.my/id/eprint/24622/1/24622.pdf
http://psasir.upm.edu.my/id/eprint/24622/7/Determination%20of%20folate%20content%20in%20commonly%20consumed%20Malaysian%20foods.pdf
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author Chew, S. C.
Loh, Su Peng
Khor, Geok Lin
author_facet Chew, S. C.
Loh, Su Peng
Khor, Geok Lin
author_sort Chew, S. C.
collection UPM
description Currently, data concerning the content of naturally occurring dietary folate in Malaysian foods is scarce. The aim of this study was to determine the folate content of vegetables, fruits, legumes and cereals that were commonly consumed among Malaysians. The total folate content of 156 samples (51 vegetables, 33 fruits, 22 legumes and legume products, and 50 cereals and cereal products) available in Malaysia was determined by microbiological assay using Lactobacillus casei (L. casei) after trienzyme treatment with protease, α-amylase and folate conjugase (from rat serum). An internal quality control system was used throughout the study by analyzing CRM 121 (wholemeal flour) and CRM 485 (lyophilized mixed vegetables); percent recovery (as mean ± SD) of 97 ± 2.0 and 101 ± 4.0 was obtained. The range of folate content in vegetables, fruits, legumes and cereals were 1-11 μg/100 g and 1-31on the basis of fresh weight and 1-31 μg/100 g and 2-156 μg/100 g on the basis of dry weight, respectively. This study has shown that some of these underutilized vegetables and fruits are good sources of folate and could fulfill the recommended dietary intake of total folate.
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spelling upm.eprints-246222015-10-02T02:58:44Z http://psasir.upm.edu.my/id/eprint/24622/ Determination of folate content in commonly consumed Malaysian foods Chew, S. C. Loh, Su Peng Khor, Geok Lin Currently, data concerning the content of naturally occurring dietary folate in Malaysian foods is scarce. The aim of this study was to determine the folate content of vegetables, fruits, legumes and cereals that were commonly consumed among Malaysians. The total folate content of 156 samples (51 vegetables, 33 fruits, 22 legumes and legume products, and 50 cereals and cereal products) available in Malaysia was determined by microbiological assay using Lactobacillus casei (L. casei) after trienzyme treatment with protease, α-amylase and folate conjugase (from rat serum). An internal quality control system was used throughout the study by analyzing CRM 121 (wholemeal flour) and CRM 485 (lyophilized mixed vegetables); percent recovery (as mean ± SD) of 97 ± 2.0 and 101 ± 4.0 was obtained. The range of folate content in vegetables, fruits, legumes and cereals were 1-11 μg/100 g and 1-31on the basis of fresh weight and 1-31 μg/100 g and 2-156 μg/100 g on the basis of dry weight, respectively. This study has shown that some of these underutilized vegetables and fruits are good sources of folate and could fulfill the recommended dietary intake of total folate. Faculty of Food Science and Technology, Universiti Putra Malaysia 2012 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24622/1/24622.pdf application/pdf en http://psasir.upm.edu.my/id/eprint/24622/7/Determination%20of%20folate%20content%20in%20commonly%20consumed%20Malaysian%20foods.pdf Chew, S. C. and Loh, Su Peng and Khor, Geok Lin (2012) Determination of folate content in commonly consumed Malaysian foods. International Food Research Journal, 19 (1). pp. 189-197. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/volume-19-2012.html English
spellingShingle Chew, S. C.
Loh, Su Peng
Khor, Geok Lin
Determination of folate content in commonly consumed Malaysian foods
title Determination of folate content in commonly consumed Malaysian foods
title_full Determination of folate content in commonly consumed Malaysian foods
title_fullStr Determination of folate content in commonly consumed Malaysian foods
title_full_unstemmed Determination of folate content in commonly consumed Malaysian foods
title_short Determination of folate content in commonly consumed Malaysian foods
title_sort determination of folate content in commonly consumed malaysian foods
url http://psasir.upm.edu.my/id/eprint/24622/1/24622.pdf
http://psasir.upm.edu.my/id/eprint/24622/7/Determination%20of%20folate%20content%20in%20commonly%20consumed%20Malaysian%20foods.pdf
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