Polyphenoloxidase from Starfruit (Averrhoa carambola, L.) I
Polyphenoloxidase (PPO) was z50lated from starfruit (Averrhoa carambola, L.) and purified by acetone precipitation and dialysis. The optimum pH for the aCl}tone purified PPO catalysed oxidation of catechol was found to be pH 7.2 Heat 'inactivation studies showed that the enzyme was heat-labi...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English English |
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1986
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| Online Access: | http://psasir.upm.edu.my/id/eprint/2470/1/Polyphenoloxidase_from_Starfruit_%28Averrhoa_carambola%2C_L.%29.pdf |
| _version_ | 1825921462292185088 |
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| author | Tengku Adnan, Tengku Adnin Augustin, M. A. Mohd Ghazali, Hasanah |
| author_facet | Tengku Adnan, Tengku Adnin Augustin, M. A. Mohd Ghazali, Hasanah |
| author_sort | Tengku Adnan, Tengku Adnin |
| collection | UPM |
| description | Polyphenoloxidase (PPO) was z50lated from starfruit (Averrhoa carambola, L.) and purified
by acetone precipitation and dialysis. The optimum pH for the aCl}tone purified PPO catalysed
oxidation of catechol was found to be pH 7.2 Heat 'inactivation studies showed that the enzyme was
heat-labile. Haif-Hvesfor PPO 'inactiivation at 70°C and 75°C were found to be 4.6 min and 3.1 min
respectively. The Michaeline constant (K j and V for catechol with PPO was O. 061M and 20 U ml-1
respectively. |
| first_indexed | 2024-03-06T06:58:16Z |
| format | Article |
| id | upm.eprints-2470 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T06:58:16Z |
| publishDate | 1986 |
| record_format | dspace |
| spelling | upm.eprints-24702013-05-27T07:01:18Z http://psasir.upm.edu.my/id/eprint/2470/ Polyphenoloxidase from Starfruit (Averrhoa carambola, L.) I Tengku Adnan, Tengku Adnin Augustin, M. A. Mohd Ghazali, Hasanah Polyphenoloxidase (PPO) was z50lated from starfruit (Averrhoa carambola, L.) and purified by acetone precipitation and dialysis. The optimum pH for the aCl}tone purified PPO catalysed oxidation of catechol was found to be pH 7.2 Heat 'inactivation studies showed that the enzyme was heat-labile. Haif-Hvesfor PPO 'inactiivation at 70°C and 75°C were found to be 4.6 min and 3.1 min respectively. The Michaeline constant (K j and V for catechol with PPO was O. 061M and 20 U ml-1 respectively. 1986 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/2470/1/Polyphenoloxidase_from_Starfruit_%28Averrhoa_carambola%2C_L.%29.pdf Tengku Adnan, Tengku Adnin and Augustin, M. A. and Mohd Ghazali, Hasanah (1986) Polyphenoloxidase from Starfruit (Averrhoa carambola, L.) I. Pertanika, 9 (2). pp. 219-224. English |
| spellingShingle | Tengku Adnan, Tengku Adnin Augustin, M. A. Mohd Ghazali, Hasanah Polyphenoloxidase from Starfruit (Averrhoa carambola, L.) I |
| title | Polyphenoloxidase from Starfruit (Averrhoa carambola, L.) I |
| title_full | Polyphenoloxidase from Starfruit (Averrhoa carambola, L.) I |
| title_fullStr | Polyphenoloxidase from Starfruit (Averrhoa carambola, L.) I |
| title_full_unstemmed | Polyphenoloxidase from Starfruit (Averrhoa carambola, L.) I |
| title_short | Polyphenoloxidase from Starfruit (Averrhoa carambola, L.) I |
| title_sort | polyphenoloxidase from starfruit averrhoa carambola l i |
| url | http://psasir.upm.edu.my/id/eprint/2470/1/Polyphenoloxidase_from_Starfruit_%28Averrhoa_carambola%2C_L.%29.pdf |
| work_keys_str_mv | AT tengkuadnantengkuadnin polyphenoloxidasefromstarfruitaverrhoacarambolali AT augustinma polyphenoloxidasefromstarfruitaverrhoacarambolali AT mohdghazalihasanah polyphenoloxidasefromstarfruitaverrhoacarambolali |