Basic fibroblast growth factor modulates cell cycle of human umbilical cord-derived mesenchymal stem cells.
Background: Mesenchymal stem cells (MSC) have great potential in regenerative medicine, immunotherapy and gene therapy due to their unique properties of self-renewal, high plasticity, immune modulation and ease for genetic modification. However, production of MSC at sufficient clinical scale remains...
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| Format: | Article |
| Language: | English English |
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Wiley-Blackwell
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/24714/1/Basic%20fibroblast%20growth%20factor%20modulates%20cell%20cycle%20of%20human%20umbilical%20cord.pdf |
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| author | Ramasamy, Rajesh Tong, C. K. Yip, W. K. Vellasamy, S. Tan, B. C. Seow, Heng Fong |
| author_facet | Ramasamy, Rajesh Tong, C. K. Yip, W. K. Vellasamy, S. Tan, B. C. Seow, Heng Fong |
| author_sort | Ramasamy, Rajesh |
| collection | UPM |
| description | Background: Mesenchymal stem cells (MSC) have great potential in regenerative medicine, immunotherapy and gene therapy due to their unique properties of self-renewal, high plasticity, immune modulation and ease for genetic modification. However, production of MSC at sufficient clinical scale remains an issue as in vitro generation of MSC inadequately fulfils the demand with respect to patients. Objectives: This study has aimed to establish optimum conditions to generate and characterize MSC from human umbilical cord (UC-MSC). Materials and methods: To optimize MSC population growth, basic fibroblast growth factor (bFGF) was utilized in culture media. Effects of bFGF on expansion kinetics, cell cycle, survival of UC-MSC, cytokine secretion, expression of early stem-cell markers and immunomodulation were investigated. Results: bFGF supplementation profoundly enhanced UC-MSC proliferation by reducing population doubling time without altering immunophenotype and immunomodulatory function of UC-MSC. However, cell cycle studies revealed that bFGF drove the cells into the cell cycle, as a higher proportion of cells resided in S phase and progressed into M phase. Consistent with this, bFGF was shown to promote expression of cyclin D proteins and their relevant kinases to drive UC-MSC to transverse cell cycle check points, thus, committing the cells to DNA synthesis. Furthermore, supplementation with bFGF changed the cytokine profiles of the cells and reduced their apoptotic level. Conclusion: Our study showed that bFGF supplementation of UC-MSC culture enhanced the cells' growth kinetics without compromising their nature. |
| first_indexed | 2024-03-06T08:00:40Z |
| format | Article |
| id | upm.eprints-24714 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T08:00:40Z |
| publishDate | 2012 |
| publisher | Wiley-Blackwell |
| record_format | dspace |
| spelling | upm.eprints-247142015-10-30T07:24:15Z http://psasir.upm.edu.my/id/eprint/24714/ Basic fibroblast growth factor modulates cell cycle of human umbilical cord-derived mesenchymal stem cells. Ramasamy, Rajesh Tong, C. K. Yip, W. K. Vellasamy, S. Tan, B. C. Seow, Heng Fong Background: Mesenchymal stem cells (MSC) have great potential in regenerative medicine, immunotherapy and gene therapy due to their unique properties of self-renewal, high plasticity, immune modulation and ease for genetic modification. However, production of MSC at sufficient clinical scale remains an issue as in vitro generation of MSC inadequately fulfils the demand with respect to patients. Objectives: This study has aimed to establish optimum conditions to generate and characterize MSC from human umbilical cord (UC-MSC). Materials and methods: To optimize MSC population growth, basic fibroblast growth factor (bFGF) was utilized in culture media. Effects of bFGF on expansion kinetics, cell cycle, survival of UC-MSC, cytokine secretion, expression of early stem-cell markers and immunomodulation were investigated. Results: bFGF supplementation profoundly enhanced UC-MSC proliferation by reducing population doubling time without altering immunophenotype and immunomodulatory function of UC-MSC. However, cell cycle studies revealed that bFGF drove the cells into the cell cycle, as a higher proportion of cells resided in S phase and progressed into M phase. Consistent with this, bFGF was shown to promote expression of cyclin D proteins and their relevant kinases to drive UC-MSC to transverse cell cycle check points, thus, committing the cells to DNA synthesis. Furthermore, supplementation with bFGF changed the cytokine profiles of the cells and reduced their apoptotic level. Conclusion: Our study showed that bFGF supplementation of UC-MSC culture enhanced the cells' growth kinetics without compromising their nature. Wiley-Blackwell 2012 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24714/1/Basic%20fibroblast%20growth%20factor%20modulates%20cell%20cycle%20of%20human%20umbilical%20cord.pdf Ramasamy, Rajesh and Tong, C. K. and Yip, W. K. and Vellasamy, S. and Tan, B. C. and Seow, Heng Fong (2012) Basic fibroblast growth factor modulates cell cycle of human umbilical cord-derived mesenchymal stem cells. Cell Proliferation , 45 (2). pp. 132-139. ISSN 0960-7722; ESSN:1365-2184 http://as.wiley.com/ 10.1111/j.1365-2184.2012.00808.x English |
| spellingShingle | Ramasamy, Rajesh Tong, C. K. Yip, W. K. Vellasamy, S. Tan, B. C. Seow, Heng Fong Basic fibroblast growth factor modulates cell cycle of human umbilical cord-derived mesenchymal stem cells. |
| title | Basic fibroblast growth factor modulates cell cycle of human umbilical cord-derived mesenchymal stem cells. |
| title_full | Basic fibroblast growth factor modulates cell cycle of human umbilical cord-derived mesenchymal stem cells. |
| title_fullStr | Basic fibroblast growth factor modulates cell cycle of human umbilical cord-derived mesenchymal stem cells. |
| title_full_unstemmed | Basic fibroblast growth factor modulates cell cycle of human umbilical cord-derived mesenchymal stem cells. |
| title_short | Basic fibroblast growth factor modulates cell cycle of human umbilical cord-derived mesenchymal stem cells. |
| title_sort | basic fibroblast growth factor modulates cell cycle of human umbilical cord derived mesenchymal stem cells |
| url | http://psasir.upm.edu.my/id/eprint/24714/1/Basic%20fibroblast%20growth%20factor%20modulates%20cell%20cycle%20of%20human%20umbilical%20cord.pdf |
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