Spatiotemporal regulation of multiple overlapping sense and novel natural antisense transcripts at the nrgn and camk2n1 gene loci during mouse cerebral corticogenesis.
Nrgn and Camk2n1 are highly expressed in the brain and play an important role in synaptic long-term potentiation via regulation of Ca 2 +/calmodulin-dependent protein kinase II. We have shown that the gene loci for these 2 proteins are actively transcribed in the adult cerebral cortex and feature mu...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English English |
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Oxford University Press (OUP)
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/24721/1/Spatiotemporal%20regulation%20of%20multiple%20overlapping%20sense%20and%20novel%20natural%20antisense%20transcripts%20at%20the%20nrgn%20and%20camk2n1%20gene%20loci%20during%20mouse%20cerebral%20corticogenesis.pdf |
| _version_ | 1796970616294735872 |
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| author | Ling, King Hwa Cheah, Pike See Hewitt, Chelsee Ann Beissbarth, Tim Hyde, Lavinia Smyth, Gordon K. Tan, S.S. Hahn, Christopher N. Thomas, Tim Thomas , P.Q. Scott, Hamish S. |
| author_facet | Ling, King Hwa Cheah, Pike See Hewitt, Chelsee Ann Beissbarth, Tim Hyde, Lavinia Smyth, Gordon K. Tan, S.S. Hahn, Christopher N. Thomas, Tim Thomas , P.Q. Scott, Hamish S. |
| author_sort | Ling, King Hwa |
| collection | UPM |
| description | Nrgn and Camk2n1 are highly expressed in the brain and play an important role in synaptic long-term potentiation via regulation of Ca 2 +/calmodulin-dependent protein kinase II. We have shown that the gene loci for these 2 proteins are actively transcribed in the adult cerebral cortex and feature multiple overlapping transcripts in both the sense and antisense orientations with alternative polyadenylation. These transcripts were upregulated in the adult compared with embryonic and P1.5 mouse cerebral cortices, and transcripts with different 3′ untranslated region lengths showed differing expression profiles. In situ hybridization (ISH) analysis revealed spatiotemporal regulation of the Nrgn and Camk2n1 sense and natural antisense transcripts (NATs) throughout cerebral corticogenesis. In addition, we also demonstrated that the expression of these transcripts was organ-specific. Both Nrgn and Camk2n1 sense and NATs were also upregulated in differentiating P19 teratocarcinoma cells. RNA fluorescent ISH analysis confirmed the capability of these NATs to form double-stranded RNA aggregates with the sense transcripts in the cytoplasm of cells obtained from the brain. We propose that the differential regulation of multiple sense and novel overlapping NATs at the Nrgn and Camk2n1 loci will increase the diversity of posttranscriptional regulation, resulting in cell- and time-specific regulation of their gene products during cerebral corticogenesis and function. |
| first_indexed | 2024-03-06T08:00:41Z |
| format | Article |
| id | upm.eprints-24721 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T08:00:41Z |
| publishDate | 2011 |
| publisher | Oxford University Press (OUP) |
| record_format | dspace |
| spelling | upm.eprints-247212015-10-05T02:42:24Z http://psasir.upm.edu.my/id/eprint/24721/ Spatiotemporal regulation of multiple overlapping sense and novel natural antisense transcripts at the nrgn and camk2n1 gene loci during mouse cerebral corticogenesis. Ling, King Hwa Cheah, Pike See Hewitt, Chelsee Ann Beissbarth, Tim Hyde, Lavinia Smyth, Gordon K. Tan, S.S. Hahn, Christopher N. Thomas, Tim Thomas , P.Q. Scott, Hamish S. Nrgn and Camk2n1 are highly expressed in the brain and play an important role in synaptic long-term potentiation via regulation of Ca 2 +/calmodulin-dependent protein kinase II. We have shown that the gene loci for these 2 proteins are actively transcribed in the adult cerebral cortex and feature multiple overlapping transcripts in both the sense and antisense orientations with alternative polyadenylation. These transcripts were upregulated in the adult compared with embryonic and P1.5 mouse cerebral cortices, and transcripts with different 3′ untranslated region lengths showed differing expression profiles. In situ hybridization (ISH) analysis revealed spatiotemporal regulation of the Nrgn and Camk2n1 sense and natural antisense transcripts (NATs) throughout cerebral corticogenesis. In addition, we also demonstrated that the expression of these transcripts was organ-specific. Both Nrgn and Camk2n1 sense and NATs were also upregulated in differentiating P19 teratocarcinoma cells. RNA fluorescent ISH analysis confirmed the capability of these NATs to form double-stranded RNA aggregates with the sense transcripts in the cytoplasm of cells obtained from the brain. We propose that the differential regulation of multiple sense and novel overlapping NATs at the Nrgn and Camk2n1 loci will increase the diversity of posttranscriptional regulation, resulting in cell- and time-specific regulation of their gene products during cerebral corticogenesis and function. Oxford University Press (OUP) 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24721/1/Spatiotemporal%20regulation%20of%20multiple%20overlapping%20sense%20and%20novel%20natural%20antisense%20transcripts%20at%20the%20nrgn%20and%20camk2n1%20gene%20loci%20during%20mouse%20cerebral%20corticogenesis.pdf Ling, King Hwa and Cheah, Pike See and Hewitt, Chelsee Ann and Beissbarth, Tim and Hyde, Lavinia and Smyth, Gordon K. and Tan, S.S. and Hahn, Christopher N. and Thomas, Tim and Thomas , P.Q. and Scott, Hamish S. (2011) Spatiotemporal regulation of multiple overlapping sense and novel natural antisense transcripts at the nrgn and camk2n1 gene loci during mouse cerebral corticogenesis. Cerebral Cortex , 21 (3). pp. 683-697. ISSN 1047-3211; ESSN:1460-2199 http://www.oxfordjournals.org/ 10.1093/cercor/bhq141 English |
| spellingShingle | Ling, King Hwa Cheah, Pike See Hewitt, Chelsee Ann Beissbarth, Tim Hyde, Lavinia Smyth, Gordon K. Tan, S.S. Hahn, Christopher N. Thomas, Tim Thomas , P.Q. Scott, Hamish S. Spatiotemporal regulation of multiple overlapping sense and novel natural antisense transcripts at the nrgn and camk2n1 gene loci during mouse cerebral corticogenesis. |
| title | Spatiotemporal regulation of multiple overlapping sense and novel natural antisense transcripts at the nrgn and camk2n1 gene loci during mouse cerebral corticogenesis. |
| title_full | Spatiotemporal regulation of multiple overlapping sense and novel natural antisense transcripts at the nrgn and camk2n1 gene loci during mouse cerebral corticogenesis. |
| title_fullStr | Spatiotemporal regulation of multiple overlapping sense and novel natural antisense transcripts at the nrgn and camk2n1 gene loci during mouse cerebral corticogenesis. |
| title_full_unstemmed | Spatiotemporal regulation of multiple overlapping sense and novel natural antisense transcripts at the nrgn and camk2n1 gene loci during mouse cerebral corticogenesis. |
| title_short | Spatiotemporal regulation of multiple overlapping sense and novel natural antisense transcripts at the nrgn and camk2n1 gene loci during mouse cerebral corticogenesis. |
| title_sort | spatiotemporal regulation of multiple overlapping sense and novel natural antisense transcripts at the nrgn and camk2n1 gene loci during mouse cerebral corticogenesis |
| url | http://psasir.upm.edu.my/id/eprint/24721/1/Spatiotemporal%20regulation%20of%20multiple%20overlapping%20sense%20and%20novel%20natural%20antisense%20transcripts%20at%20the%20nrgn%20and%20camk2n1%20gene%20loci%20during%20mouse%20cerebral%20corticogenesis.pdf |
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