Construction of cDNA library and preliminary analysis of expressed sequence tags from green microalga Ankistrodesmus convolutus Corda
Green microalga Ankistrodesmus convolutus Corda is a fast growing alga which produces appreciable amount of carotenoids and polyunsaturated fatty acids. To our knowledge, this is the first report on the construction of cDNA library and preliminary analysis of ESTs for this species. The titers of the...
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| Format: | Article |
| Language: | English |
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Springer
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/25181/1/Construction%20of%20cDNA%20library%20and%20preliminary%20analysis%20of%20expressed%20sequence%20tags%20from%20green%20microalga%20Ankistrodesmus%20convolutus%20Corda.pdf |
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| author | Thanh, Tran Chi, Vu Thi Quynh Abdullah, Mohd Puad Omar, Hishamuddin Noroozi, Mostafa Ky, Huynh Napis, Suhaimi |
| author_facet | Thanh, Tran Chi, Vu Thi Quynh Abdullah, Mohd Puad Omar, Hishamuddin Noroozi, Mostafa Ky, Huynh Napis, Suhaimi |
| author_sort | Thanh, Tran |
| collection | UPM |
| description | Green microalga Ankistrodesmus convolutus Corda is a fast growing alga which produces appreciable amount of carotenoids and polyunsaturated fatty acids. To our knowledge, this is the first report on the construction of cDNA library and preliminary analysis of ESTs for this species. The titers of the primary and amplified cDNA libraries were 1.1×10(6) and 6.0×10(9) pfu/ml respectively. The percentage of recombinants was 97% in the primary library and a total of 337 out of 415 original cDNA clones selected randomly contained inserts ranging from 600 to 1,500 bps. A total of 201 individual ESTs with sizes ranging from 390 to 1,038 bps were then analyzed and the BLASTX score revealed that 35.8% of the sequences were classified as strong match, 38.3% as nominal and 25.9% as weak match. Among the ESTs with known putative function, 21.4% of them were found to be related to gene expression, 14.4% ESTs to photosynthesis, 10.9% ESTs to metabolism, 5.5% ESTs to miscellaneous, 2.0% to stress response, and the remaining 45.8% were classified as novel genes. Analysis of ESTs described in this paper can be an effective approach to isolate and characterize new genes from A. convolutus and thus the sequences obtained represented a significant contribution to the extensive database of sequences from green microalgae. |
| first_indexed | 2024-03-06T08:02:04Z |
| format | Article |
| id | upm.eprints-25181 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:02:04Z |
| publishDate | 2011 |
| publisher | Springer |
| record_format | dspace |
| spelling | upm.eprints-251812017-09-28T08:06:48Z http://psasir.upm.edu.my/id/eprint/25181/ Construction of cDNA library and preliminary analysis of expressed sequence tags from green microalga Ankistrodesmus convolutus Corda Thanh, Tran Chi, Vu Thi Quynh Abdullah, Mohd Puad Omar, Hishamuddin Noroozi, Mostafa Ky, Huynh Napis, Suhaimi Green microalga Ankistrodesmus convolutus Corda is a fast growing alga which produces appreciable amount of carotenoids and polyunsaturated fatty acids. To our knowledge, this is the first report on the construction of cDNA library and preliminary analysis of ESTs for this species. The titers of the primary and amplified cDNA libraries were 1.1×10(6) and 6.0×10(9) pfu/ml respectively. The percentage of recombinants was 97% in the primary library and a total of 337 out of 415 original cDNA clones selected randomly contained inserts ranging from 600 to 1,500 bps. A total of 201 individual ESTs with sizes ranging from 390 to 1,038 bps were then analyzed and the BLASTX score revealed that 35.8% of the sequences were classified as strong match, 38.3% as nominal and 25.9% as weak match. Among the ESTs with known putative function, 21.4% of them were found to be related to gene expression, 14.4% ESTs to photosynthesis, 10.9% ESTs to metabolism, 5.5% ESTs to miscellaneous, 2.0% to stress response, and the remaining 45.8% were classified as novel genes. Analysis of ESTs described in this paper can be an effective approach to isolate and characterize new genes from A. convolutus and thus the sequences obtained represented a significant contribution to the extensive database of sequences from green microalgae. Springer 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/25181/1/Construction%20of%20cDNA%20library%20and%20preliminary%20analysis%20of%20expressed%20sequence%20tags%20from%20green%20microalga%20Ankistrodesmus%20convolutus%20Corda.pdf Thanh, Tran and Chi, Vu Thi Quynh and Abdullah, Mohd Puad and Omar, Hishamuddin and Noroozi, Mostafa and Ky, Huynh and Napis, Suhaimi (2011) Construction of cDNA library and preliminary analysis of expressed sequence tags from green microalga Ankistrodesmus convolutus Corda. Molecular Biology Reports, 38 (1). pp. 177-182. ISSN 0301-4851; ESSN: 1573-4978 https://rd.springer.com/article/10.1007%2Fs11033-010-0092-4 10.1007/s11033-010-0092-4 |
| spellingShingle | Thanh, Tran Chi, Vu Thi Quynh Abdullah, Mohd Puad Omar, Hishamuddin Noroozi, Mostafa Ky, Huynh Napis, Suhaimi Construction of cDNA library and preliminary analysis of expressed sequence tags from green microalga Ankistrodesmus convolutus Corda |
| title | Construction of cDNA library and preliminary analysis of expressed sequence tags from green microalga Ankistrodesmus convolutus Corda |
| title_full | Construction of cDNA library and preliminary analysis of expressed sequence tags from green microalga Ankistrodesmus convolutus Corda |
| title_fullStr | Construction of cDNA library and preliminary analysis of expressed sequence tags from green microalga Ankistrodesmus convolutus Corda |
| title_full_unstemmed | Construction of cDNA library and preliminary analysis of expressed sequence tags from green microalga Ankistrodesmus convolutus Corda |
| title_short | Construction of cDNA library and preliminary analysis of expressed sequence tags from green microalga Ankistrodesmus convolutus Corda |
| title_sort | construction of cdna library and preliminary analysis of expressed sequence tags from green microalga ankistrodesmus convolutus corda |
| url | http://psasir.upm.edu.my/id/eprint/25181/1/Construction%20of%20cDNA%20library%20and%20preliminary%20analysis%20of%20expressed%20sequence%20tags%20from%20green%20microalga%20Ankistrodesmus%20convolutus%20Corda.pdf |
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