Assessment of sensitivity, specificity and species discriminatory power of four culture-based isolation methods of Arcobacter spp
A total of 142 samples including beef (n = 52), fresh milk (n = 45) and rectal swabs of cattle (n = 45) were collected from various wet markets (beef) and dairy cattle farms (milk and rectal swabs) in the vicinity of Selangor, Malaysia. All samples were examined for the presence of Arcobacter specie...
| Päätekijät: | , , , |
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| Aineistotyyppi: | Artikkeli |
| Kieli: | English |
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Academic Journals
2011
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| Linkit: | http://psasir.upm.edu.my/id/eprint/25319/1/25319.pdf |
| _version_ | 1825925754699907072 |
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| author | Hussain Shah, Sayed Atta Abdul Aziz, Saleha Marimuthu, Murugaiyah Zakaria, Zunita |
| author_facet | Hussain Shah, Sayed Atta Abdul Aziz, Saleha Marimuthu, Murugaiyah Zakaria, Zunita |
| author_sort | Hussain Shah, Sayed Atta |
| collection | UPM |
| description | A total of 142 samples including beef (n = 52), fresh milk (n = 45) and rectal swabs of cattle (n = 45) were collected from various wet markets (beef) and dairy cattle farms (milk and rectal swabs) in the vicinity of Selangor, Malaysia. All samples were examined for the presence of Arcobacter species using four different isolation methods. The organisms were first identified on the basis of phenotypic tests and later the suspected isolates were confirmed using multiplex PCR (mPCR). Method I (MI) and Method II (MII) detected Arcobacter in 43.7 and 75%, respectively from beef, but were unable to detect microbe from milk and rectal swabs. On the other hand, Method III (MIII) detected Arcobacter in 100, 60 and 40% of beef, milk and bovine rectal swab samples respectively; Method IV (MIV) detected 93.7, 60 and 40% in beef, milk and cattle rectal swabs respectively. The discriminatory power among the isolation methods for Arcobacter species was evaluated and MIII was found to be the best as it identified A. butzleri (72.7%); A. cryaerophilus (22.7%) and A. skirrowii (4.5%) while A. butzleri (61.1%), A. cryaerophilus (22.7%) and A. skirrowii (9%) were isolated from MIV. |
| first_indexed | 2024-03-06T08:02:29Z |
| format | Article |
| id | upm.eprints-25319 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:02:29Z |
| publishDate | 2011 |
| publisher | Academic Journals |
| record_format | dspace |
| spelling | upm.eprints-253192016-11-22T09:07:57Z http://psasir.upm.edu.my/id/eprint/25319/ Assessment of sensitivity, specificity and species discriminatory power of four culture-based isolation methods of Arcobacter spp Hussain Shah, Sayed Atta Abdul Aziz, Saleha Marimuthu, Murugaiyah Zakaria, Zunita A total of 142 samples including beef (n = 52), fresh milk (n = 45) and rectal swabs of cattle (n = 45) were collected from various wet markets (beef) and dairy cattle farms (milk and rectal swabs) in the vicinity of Selangor, Malaysia. All samples were examined for the presence of Arcobacter species using four different isolation methods. The organisms were first identified on the basis of phenotypic tests and later the suspected isolates were confirmed using multiplex PCR (mPCR). Method I (MI) and Method II (MII) detected Arcobacter in 43.7 and 75%, respectively from beef, but were unable to detect microbe from milk and rectal swabs. On the other hand, Method III (MIII) detected Arcobacter in 100, 60 and 40% of beef, milk and bovine rectal swab samples respectively; Method IV (MIV) detected 93.7, 60 and 40% in beef, milk and cattle rectal swabs respectively. The discriminatory power among the isolation methods for Arcobacter species was evaluated and MIII was found to be the best as it identified A. butzleri (72.7%); A. cryaerophilus (22.7%) and A. skirrowii (4.5%) while A. butzleri (61.1%), A. cryaerophilus (22.7%) and A. skirrowii (9%) were isolated from MIV. Academic Journals 2011-10-16 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/25319/1/25319.pdf Hussain Shah, Sayed Atta and Abdul Aziz, Saleha and Marimuthu, Murugaiyah and Zakaria, Zunita (2011) Assessment of sensitivity, specificity and species discriminatory power of four culture-based isolation methods of Arcobacter spp. African Journal of Microbiology Research, 5 (22). art. no. 08A5A0513572. pp. 3753-3759. ISSN 1996-0808 http://www.academicjournals.org/journal/AJMR/article-abstract/08A5A0513572 10.5897/AJMR11.618 |
| spellingShingle | Hussain Shah, Sayed Atta Abdul Aziz, Saleha Marimuthu, Murugaiyah Zakaria, Zunita Assessment of sensitivity, specificity and species discriminatory power of four culture-based isolation methods of Arcobacter spp |
| title | Assessment of sensitivity, specificity and species discriminatory power of four culture-based isolation methods of Arcobacter spp |
| title_full | Assessment of sensitivity, specificity and species discriminatory power of four culture-based isolation methods of Arcobacter spp |
| title_fullStr | Assessment of sensitivity, specificity and species discriminatory power of four culture-based isolation methods of Arcobacter spp |
| title_full_unstemmed | Assessment of sensitivity, specificity and species discriminatory power of four culture-based isolation methods of Arcobacter spp |
| title_short | Assessment of sensitivity, specificity and species discriminatory power of four culture-based isolation methods of Arcobacter spp |
| title_sort | assessment of sensitivity specificity and species discriminatory power of four culture based isolation methods of arcobacter spp |
| url | http://psasir.upm.edu.my/id/eprint/25319/1/25319.pdf |
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