Cryotop device enhances vitrification outcome of immature Bovine Oocytes.
The aim of this study was to evaluate the effectiveness of different cryodevices (Open Pulled Straw (OPS), Electron Microscopy Grid (EMG) and cryotop for vitrification of immature bovine oocytes. Polar body, MII stage, survivability and subsequent developmental rates were compared. Only oocytes with...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
Medwell Publishing
2011
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/25354/1/Cryotop%20device%20enhances%20vitrification%20outcome%20of%20immature%20Bovine%20Oocytes.pdf |
| _version_ | 1796970741992783872 |
|---|---|
| author | Hajarian, Hadi Haron, Wahid Yusoff, Rosnina Morteza, Daliri Mojtaba, Dashtizad Mirzapour, Tooba Yimer, Nurhusien Muhammad Modu, Bukar Ismail, Mohd Iswadi Othman, Abas Mazni |
| author_facet | Hajarian, Hadi Haron, Wahid Yusoff, Rosnina Morteza, Daliri Mojtaba, Dashtizad Mirzapour, Tooba Yimer, Nurhusien Muhammad Modu, Bukar Ismail, Mohd Iswadi Othman, Abas Mazni |
| author_sort | Hajarian, Hadi |
| collection | UPM |
| description | The aim of this study was to evaluate the effectiveness of different cryodevices (Open Pulled Straw (OPS), Electron Microscopy Grid (EMG) and cryotop for vitrification of immature bovine oocytes. Polar body, MII stage, survivability and subsequent developmental rates were compared. Only oocytes with 4-5 layers of cumulus cells were used. Oocytes were equilibrated in the first vitrification solution (VS1; HS+10% DMSO+10% Ethylene Glycol (EG)) for 30-45 sec and then in the second vitrification solution (VS2; 20% DMSO+20% EG+0.5 M Sucrose) for 25 sec. Within 30 sec they were mounted on one of the cryodevices and directly plunged into Liquid Nitrogen (LN2) for 10 days. Immature oocytes vitrified using cryotop represented higher rate of polar body extrusion and nuclear maturity (p<0.05). The highest survivability resulted from cryotop and EMG groups and no significant difference found between them. Vitrified oocytes in cryotop group had highest cleavage and blastocyst rates. All of the mean measured rates for vitrified/warmed immature oocytes were significantly lower than that of control group (p<0.05). In conclusion, results of this study showed the superiority of cryotop device for vitrification of immature bovine oocytes which resulted in higher viability and subsequent embryo development. |
| first_indexed | 2024-03-06T08:02:35Z |
| format | Article |
| id | upm.eprints-25354 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T08:02:35Z |
| publishDate | 2011 |
| publisher | Medwell Publishing |
| record_format | dspace |
| spelling | upm.eprints-253542015-09-14T01:59:39Z http://psasir.upm.edu.my/id/eprint/25354/ Cryotop device enhances vitrification outcome of immature Bovine Oocytes. Hajarian, Hadi Haron, Wahid Yusoff, Rosnina Morteza, Daliri Mojtaba, Dashtizad Mirzapour, Tooba Yimer, Nurhusien Muhammad Modu, Bukar Ismail, Mohd Iswadi Othman, Abas Mazni The aim of this study was to evaluate the effectiveness of different cryodevices (Open Pulled Straw (OPS), Electron Microscopy Grid (EMG) and cryotop for vitrification of immature bovine oocytes. Polar body, MII stage, survivability and subsequent developmental rates were compared. Only oocytes with 4-5 layers of cumulus cells were used. Oocytes were equilibrated in the first vitrification solution (VS1; HS+10% DMSO+10% Ethylene Glycol (EG)) for 30-45 sec and then in the second vitrification solution (VS2; 20% DMSO+20% EG+0.5 M Sucrose) for 25 sec. Within 30 sec they were mounted on one of the cryodevices and directly plunged into Liquid Nitrogen (LN2) for 10 days. Immature oocytes vitrified using cryotop represented higher rate of polar body extrusion and nuclear maturity (p<0.05). The highest survivability resulted from cryotop and EMG groups and no significant difference found between them. Vitrified oocytes in cryotop group had highest cleavage and blastocyst rates. All of the mean measured rates for vitrified/warmed immature oocytes were significantly lower than that of control group (p<0.05). In conclusion, results of this study showed the superiority of cryotop device for vitrification of immature bovine oocytes which resulted in higher viability and subsequent embryo development. Medwell Publishing 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/25354/1/Cryotop%20device%20enhances%20vitrification%20outcome%20of%20immature%20Bovine%20Oocytes.pdf Hajarian, Hadi and Haron, Wahid and Yusoff, Rosnina and Morteza, Daliri and Mojtaba, Dashtizad and Mirzapour, Tooba and Yimer, Nurhusien and Muhammad Modu, Bukar and Ismail, Mohd Iswadi and Othman, Abas Mazni (2011) Cryotop device enhances vitrification outcome of immature Bovine Oocytes. Journal of Animal and Veterinary Advances, 10 (19). pp. 2541-2545. ISSN 1680-5593 10.3923/javaa.2011.2541.2545 English |
| spellingShingle | Hajarian, Hadi Haron, Wahid Yusoff, Rosnina Morteza, Daliri Mojtaba, Dashtizad Mirzapour, Tooba Yimer, Nurhusien Muhammad Modu, Bukar Ismail, Mohd Iswadi Othman, Abas Mazni Cryotop device enhances vitrification outcome of immature Bovine Oocytes. |
| title | Cryotop device enhances vitrification outcome of immature Bovine Oocytes. |
| title_full | Cryotop device enhances vitrification outcome of immature Bovine Oocytes. |
| title_fullStr | Cryotop device enhances vitrification outcome of immature Bovine Oocytes. |
| title_full_unstemmed | Cryotop device enhances vitrification outcome of immature Bovine Oocytes. |
| title_short | Cryotop device enhances vitrification outcome of immature Bovine Oocytes. |
| title_sort | cryotop device enhances vitrification outcome of immature bovine oocytes |
| url | http://psasir.upm.edu.my/id/eprint/25354/1/Cryotop%20device%20enhances%20vitrification%20outcome%20of%20immature%20Bovine%20Oocytes.pdf |
| work_keys_str_mv | AT hajarianhadi cryotopdeviceenhancesvitrificationoutcomeofimmaturebovineoocytes AT haronwahid cryotopdeviceenhancesvitrificationoutcomeofimmaturebovineoocytes AT yusoffrosnina cryotopdeviceenhancesvitrificationoutcomeofimmaturebovineoocytes AT mortezadaliri cryotopdeviceenhancesvitrificationoutcomeofimmaturebovineoocytes AT mojtabadashtizad cryotopdeviceenhancesvitrificationoutcomeofimmaturebovineoocytes AT mirzapourtooba cryotopdeviceenhancesvitrificationoutcomeofimmaturebovineoocytes AT yimernurhusien cryotopdeviceenhancesvitrificationoutcomeofimmaturebovineoocytes AT muhammadmodubukar cryotopdeviceenhancesvitrificationoutcomeofimmaturebovineoocytes AT ismailmohdiswadi cryotopdeviceenhancesvitrificationoutcomeofimmaturebovineoocytes AT othmanabasmazni cryotopdeviceenhancesvitrificationoutcomeofimmaturebovineoocytes |