Effect of donor cells concentration on colonization of human spermatogonial stem cells in recipient mouse testes.
Exogenesis (cross-species) germ cell transplantation provides an opportunity to investigate fundamental aspects of spermatogenesis. In this study, testis biopsies of patients with maturation arrest of spermatogenesis during a one year ago were first minced mechanically into small pieces and then Spe...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
Asian Network for Scientific Information
2010
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/25580/1/Effect%20of%20donor%20cells%20concentration%20on%20colonization%20of%20human%20spermatogonial%20stem%20cells%20in%20recipient%20mouse%20testes.pdf |
| _version_ | 1825925803042406400 |
|---|---|
| author | Mirzapour, Tooba Movahedin, Mansoureh Tengku Ibrahim, Tengku Azmi Haron, Abdul Wahid Makoolati , Zohreh Nowroozi, Mohamadreza |
| author_facet | Mirzapour, Tooba Movahedin, Mansoureh Tengku Ibrahim, Tengku Azmi Haron, Abdul Wahid Makoolati , Zohreh Nowroozi, Mohamadreza |
| author_sort | Mirzapour, Tooba |
| collection | UPM |
| description | Exogenesis (cross-species) germ cell transplantation provides an opportunity to investigate fundamental aspects of spermatogenesis. In this study, testis biopsies of patients with maturation arrest of spermatogenesis during a one year ago were first minced mechanically into small pieces and then Spermatogonial Stem Cells (SSCs) and Sertoli cells isolated by the two- step enzymatic digestion, were plated and grown on DSA-Lectin coated dishes in Dulbecco's Modified Eagle's Medium (DMEM) containing 10% fetal calf serum. Transplantation of human spermatogonial cells into mouse recipient testis was performed on day 7 (before colony formation) and 2 weeks after culturing (colony formation). The effects of different concentrations of spermatogonial cell on quantity of transplantation and percent of colonized seminiferous tubules were assayed during 8 weeks after transplantation. The result showed that SSCs can be observed on the basement membrane of the seminiferous tubules in place of spermatogonial stem cells and proliferation occurs about 4 weeks after transplantation. The difference in donor cells concentration had more effect on colonization of mouse recipient testis (p<0.05). It will be an alternative approach for the repopulation of infertile seminiferous tubules and preservation of fertility, in the future |
| first_indexed | 2024-03-06T08:03:10Z |
| format | Article |
| id | upm.eprints-25580 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T08:03:10Z |
| publishDate | 2010 |
| publisher | Asian Network for Scientific Information |
| record_format | dspace |
| spelling | upm.eprints-255802015-10-21T00:49:49Z http://psasir.upm.edu.my/id/eprint/25580/ Effect of donor cells concentration on colonization of human spermatogonial stem cells in recipient mouse testes. Mirzapour, Tooba Movahedin, Mansoureh Tengku Ibrahim, Tengku Azmi Haron, Abdul Wahid Makoolati , Zohreh Nowroozi, Mohamadreza Exogenesis (cross-species) germ cell transplantation provides an opportunity to investigate fundamental aspects of spermatogenesis. In this study, testis biopsies of patients with maturation arrest of spermatogenesis during a one year ago were first minced mechanically into small pieces and then Spermatogonial Stem Cells (SSCs) and Sertoli cells isolated by the two- step enzymatic digestion, were plated and grown on DSA-Lectin coated dishes in Dulbecco's Modified Eagle's Medium (DMEM) containing 10% fetal calf serum. Transplantation of human spermatogonial cells into mouse recipient testis was performed on day 7 (before colony formation) and 2 weeks after culturing (colony formation). The effects of different concentrations of spermatogonial cell on quantity of transplantation and percent of colonized seminiferous tubules were assayed during 8 weeks after transplantation. The result showed that SSCs can be observed on the basement membrane of the seminiferous tubules in place of spermatogonial stem cells and proliferation occurs about 4 weeks after transplantation. The difference in donor cells concentration had more effect on colonization of mouse recipient testis (p<0.05). It will be an alternative approach for the repopulation of infertile seminiferous tubules and preservation of fertility, in the future Asian Network for Scientific Information 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/25580/1/Effect%20of%20donor%20cells%20concentration%20on%20colonization%20of%20human%20spermatogonial%20stem%20cells%20in%20recipient%20mouse%20testes.pdf Mirzapour, Tooba and Movahedin, Mansoureh and Tengku Ibrahim, Tengku Azmi and Haron, Abdul Wahid and Makoolati , Zohreh and Nowroozi, Mohamadreza (2010) Effect of donor cells concentration on colonization of human spermatogonial stem cells in recipient mouse testes. Journal of Biological Sciences, 10 (8). pp. 730-738. ISSN 1727-3048 10.3923/jbs.2010.730.738 English |
| spellingShingle | Mirzapour, Tooba Movahedin, Mansoureh Tengku Ibrahim, Tengku Azmi Haron, Abdul Wahid Makoolati , Zohreh Nowroozi, Mohamadreza Effect of donor cells concentration on colonization of human spermatogonial stem cells in recipient mouse testes. |
| title | Effect of donor cells concentration on colonization of human spermatogonial stem cells in recipient mouse testes. |
| title_full | Effect of donor cells concentration on colonization of human spermatogonial stem cells in recipient mouse testes. |
| title_fullStr | Effect of donor cells concentration on colonization of human spermatogonial stem cells in recipient mouse testes. |
| title_full_unstemmed | Effect of donor cells concentration on colonization of human spermatogonial stem cells in recipient mouse testes. |
| title_short | Effect of donor cells concentration on colonization of human spermatogonial stem cells in recipient mouse testes. |
| title_sort | effect of donor cells concentration on colonization of human spermatogonial stem cells in recipient mouse testes |
| url | http://psasir.upm.edu.my/id/eprint/25580/1/Effect%20of%20donor%20cells%20concentration%20on%20colonization%20of%20human%20spermatogonial%20stem%20cells%20in%20recipient%20mouse%20testes.pdf |
| work_keys_str_mv | AT mirzapourtooba effectofdonorcellsconcentrationoncolonizationofhumanspermatogonialstemcellsinrecipientmousetestes AT movahedinmansoureh effectofdonorcellsconcentrationoncolonizationofhumanspermatogonialstemcellsinrecipientmousetestes AT tengkuibrahimtengkuazmi effectofdonorcellsconcentrationoncolonizationofhumanspermatogonialstemcellsinrecipientmousetestes AT haronabdulwahid effectofdonorcellsconcentrationoncolonizationofhumanspermatogonialstemcellsinrecipientmousetestes AT makoolatizohreh effectofdonorcellsconcentrationoncolonizationofhumanspermatogonialstemcellsinrecipientmousetestes AT nowroozimohamadreza effectofdonorcellsconcentrationoncolonizationofhumanspermatogonialstemcellsinrecipientmousetestes |