Detection of tdh and trh genes in Vibrio parahaemolyticus isolated from Corbicula moltkiana prime in West Sumatera, Indonesia.
The occurrence of Vibrio parahaemolyticus in raw Corbicula moltkiana Prime from Lake Singkarak and Pasar Raya Padang market and in cooked samples in West Sumatera, Indonesia, was studied. Thirteen raw and seven cooked bivalve samples were positive using CHROMAgar Vibrio medium. All 47 V parahaemolyt...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
Southeast Asian Ministers of Education Organisation
2007
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/25622/1/Detection%20of%20tdh%20and%20trh%20genes%20in%20Vibrio%20parahaemolyticus%20isolated%20from%20Corbicula%20moltkiana%20prime%20in%20West%20Sumatera.pdf |
| _version_ | 1825925811115393024 |
|---|---|
| author | Marlina, Radu, Son Cheah, Yoke Kqueen Napis, Suhaimi Zakaria, Zunita Abd Mutalib, Sahilah Nishibuchi, Mitsuaki |
| author_facet | Marlina, Radu, Son Cheah, Yoke Kqueen Napis, Suhaimi Zakaria, Zunita Abd Mutalib, Sahilah Nishibuchi, Mitsuaki |
| author_sort | Marlina, |
| collection | UPM |
| description | The occurrence of Vibrio parahaemolyticus in raw Corbicula moltkiana Prime from Lake Singkarak and Pasar Raya Padang market and in cooked samples in West Sumatera, Indonesia, was studied. Thirteen raw and seven cooked bivalve samples were positive using CHROMAgar Vibrio medium. All 47 V parahaemolyticus isolates were positive for toxR gene but negative for trh. However, 36% (17/47) of V parahaemolyticus strains were positive for tdh gene. Antibiotic profiling showed that 76% and 38% of isolates from raw and cooked bivalves respectively were resistant to ampicillin. Using RAPD-PCR analysis, most of the strains were clustered according to their source of isolation but some of the strains from raw and cooked samples were clustered together. These results indicate that pathogenic V parahaemolyticus isolates are present in Corbicula moltkiana Prime in West Sumatera, Indonesia, suggesting that V parahaemolyticus may also be present in seafood in other regions of Indonesia. |
| first_indexed | 2024-03-06T08:03:17Z |
| format | Article |
| id | upm.eprints-25622 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T08:03:17Z |
| publishDate | 2007 |
| publisher | Southeast Asian Ministers of Education Organisation |
| record_format | dspace |
| spelling | upm.eprints-256222015-10-26T01:59:10Z http://psasir.upm.edu.my/id/eprint/25622/ Detection of tdh and trh genes in Vibrio parahaemolyticus isolated from Corbicula moltkiana prime in West Sumatera, Indonesia. Marlina, Radu, Son Cheah, Yoke Kqueen Napis, Suhaimi Zakaria, Zunita Abd Mutalib, Sahilah Nishibuchi, Mitsuaki The occurrence of Vibrio parahaemolyticus in raw Corbicula moltkiana Prime from Lake Singkarak and Pasar Raya Padang market and in cooked samples in West Sumatera, Indonesia, was studied. Thirteen raw and seven cooked bivalve samples were positive using CHROMAgar Vibrio medium. All 47 V parahaemolyticus isolates were positive for toxR gene but negative for trh. However, 36% (17/47) of V parahaemolyticus strains were positive for tdh gene. Antibiotic profiling showed that 76% and 38% of isolates from raw and cooked bivalves respectively were resistant to ampicillin. Using RAPD-PCR analysis, most of the strains were clustered according to their source of isolation but some of the strains from raw and cooked samples were clustered together. These results indicate that pathogenic V parahaemolyticus isolates are present in Corbicula moltkiana Prime in West Sumatera, Indonesia, suggesting that V parahaemolyticus may also be present in seafood in other regions of Indonesia. Southeast Asian Ministers of Education Organisation 2007-03 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/25622/1/Detection%20of%20tdh%20and%20trh%20genes%20in%20Vibrio%20parahaemolyticus%20isolated%20from%20Corbicula%20moltkiana%20prime%20in%20West%20Sumatera.pdf Marlina, and Radu, Son and Cheah, Yoke Kqueen and Napis, Suhaimi and Zakaria, Zunita and Abd Mutalib, Sahilah and Nishibuchi, Mitsuaki (2007) Detection of tdh and trh genes in Vibrio parahaemolyticus isolated from Corbicula moltkiana prime in West Sumatera, Indonesia. Southeast Asian Journal of Tropical Medicine and Public Health, 38 (2). pp. 349-355. ISSN 0125-1562 http://www.tm.mahidol.ac.th/seameo/2007_38_2/19-3882.pdf English |
| spellingShingle | Marlina, Radu, Son Cheah, Yoke Kqueen Napis, Suhaimi Zakaria, Zunita Abd Mutalib, Sahilah Nishibuchi, Mitsuaki Detection of tdh and trh genes in Vibrio parahaemolyticus isolated from Corbicula moltkiana prime in West Sumatera, Indonesia. |
| title | Detection of tdh and trh genes in Vibrio parahaemolyticus isolated from Corbicula moltkiana prime in West Sumatera, Indonesia. |
| title_full | Detection of tdh and trh genes in Vibrio parahaemolyticus isolated from Corbicula moltkiana prime in West Sumatera, Indonesia. |
| title_fullStr | Detection of tdh and trh genes in Vibrio parahaemolyticus isolated from Corbicula moltkiana prime in West Sumatera, Indonesia. |
| title_full_unstemmed | Detection of tdh and trh genes in Vibrio parahaemolyticus isolated from Corbicula moltkiana prime in West Sumatera, Indonesia. |
| title_short | Detection of tdh and trh genes in Vibrio parahaemolyticus isolated from Corbicula moltkiana prime in West Sumatera, Indonesia. |
| title_sort | detection of tdh and trh genes in vibrio parahaemolyticus isolated from corbicula moltkiana prime in west sumatera indonesia |
| url | http://psasir.upm.edu.my/id/eprint/25622/1/Detection%20of%20tdh%20and%20trh%20genes%20in%20Vibrio%20parahaemolyticus%20isolated%20from%20Corbicula%20moltkiana%20prime%20in%20West%20Sumatera.pdf |
| work_keys_str_mv | AT marlina detectionoftdhandtrhgenesinvibrioparahaemolyticusisolatedfromcorbiculamoltkianaprimeinwestsumateraindonesia AT raduson detectionoftdhandtrhgenesinvibrioparahaemolyticusisolatedfromcorbiculamoltkianaprimeinwestsumateraindonesia AT cheahyokekqueen detectionoftdhandtrhgenesinvibrioparahaemolyticusisolatedfromcorbiculamoltkianaprimeinwestsumateraindonesia AT napissuhaimi detectionoftdhandtrhgenesinvibrioparahaemolyticusisolatedfromcorbiculamoltkianaprimeinwestsumateraindonesia AT zakariazunita detectionoftdhandtrhgenesinvibrioparahaemolyticusisolatedfromcorbiculamoltkianaprimeinwestsumateraindonesia AT abdmutalibsahilah detectionoftdhandtrhgenesinvibrioparahaemolyticusisolatedfromcorbiculamoltkianaprimeinwestsumateraindonesia AT nishibuchimitsuaki detectionoftdhandtrhgenesinvibrioparahaemolyticusisolatedfromcorbiculamoltkianaprimeinwestsumateraindonesia |