Presence of vancomycin resistance among enterococcus isolates from stray cats in Universiti Putra Malaysia and selected neighbourhood in Sri Serdang, Selangor, Malaysia
The present study was undertaken to determine the distribution of Enterococcus species from stray cats in Universiti Putra Malaysia and Seri Serdang and the presence of vancomycin resistance among the isolates. Fifty-five rectal swabs were collected from stray cats found in UPM and around the area o...
| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/27543/1/Proceedings-2.pdf |
| _version_ | 1796971154232049664 |
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| author | Zulkifli, Nurul Husna Hassan, Latiffah Zakaria, Zunita |
| author_facet | Zulkifli, Nurul Husna Hassan, Latiffah Zakaria, Zunita |
| author_sort | Zulkifli, Nurul Husna |
| collection | UPM |
| description | The present study was undertaken to determine the distribution of Enterococcus species from stray cats in Universiti Putra Malaysia and Seri Serdang and the presence of vancomycin resistance among the isolates. Fifty-five rectal swabs were collected from stray cats found in UPM and around the area of Sri Serdang. The Enterococcus species isolated were inoculated onto vancomycin resistant enterococci (VRE) agar supplemented with 8 μg/mL of vancomycin. Biochemical tests such as catalase, bile-aesculin and 6.5% NaCI were conducted to further confirm VRE isolates. Multiplex polymerase chain reaction assay (PCR) were performed for Enterococcus genus and species identification and vancomycin-resistant gene detection. Presence of Enterococcus spp. were demonstrated in every rectal sample tested. Two species were identified: Enterococcus faecalis (E. faecalis) and Enterococcus faecium (E. faecium). Among 55 isolates of Enterococcus tested, none was resistant to vancomycin at 8 μg/mL. |
| first_indexed | 2024-03-06T08:08:45Z |
| format | Conference or Workshop Item |
| id | upm.eprints-27543 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:08:45Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | upm.eprints-275432018-10-12T01:38:08Z http://psasir.upm.edu.my/id/eprint/27543/ Presence of vancomycin resistance among enterococcus isolates from stray cats in Universiti Putra Malaysia and selected neighbourhood in Sri Serdang, Selangor, Malaysia Zulkifli, Nurul Husna Hassan, Latiffah Zakaria, Zunita The present study was undertaken to determine the distribution of Enterococcus species from stray cats in Universiti Putra Malaysia and Seri Serdang and the presence of vancomycin resistance among the isolates. Fifty-five rectal swabs were collected from stray cats found in UPM and around the area of Sri Serdang. The Enterococcus species isolated were inoculated onto vancomycin resistant enterococci (VRE) agar supplemented with 8 μg/mL of vancomycin. Biochemical tests such as catalase, bile-aesculin and 6.5% NaCI were conducted to further confirm VRE isolates. Multiplex polymerase chain reaction assay (PCR) were performed for Enterococcus genus and species identification and vancomycin-resistant gene detection. Presence of Enterococcus spp. were demonstrated in every rectal sample tested. Two species were identified: Enterococcus faecalis (E. faecalis) and Enterococcus faecium (E. faecium). Among 55 isolates of Enterococcus tested, none was resistant to vancomycin at 8 μg/mL. 2010-01-05 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/27543/1/Proceedings-2.pdf Zulkifli, Nurul Husna and Hassan, Latiffah and Zakaria, Zunita (2010) Presence of vancomycin resistance among enterococcus isolates from stray cats in Universiti Putra Malaysia and selected neighbourhood in Sri Serdang, Selangor, Malaysia. In: 5th Seminar on Veterinary Sciences, 5-8 Jan. 2010, Serdang, Selangor. (pp. 7-11). |
| spellingShingle | Zulkifli, Nurul Husna Hassan, Latiffah Zakaria, Zunita Presence of vancomycin resistance among enterococcus isolates from stray cats in Universiti Putra Malaysia and selected neighbourhood in Sri Serdang, Selangor, Malaysia |
| title | Presence of vancomycin resistance among enterococcus isolates from stray cats in Universiti Putra Malaysia and selected neighbourhood in Sri Serdang, Selangor, Malaysia |
| title_full | Presence of vancomycin resistance among enterococcus isolates from stray cats in Universiti Putra Malaysia and selected neighbourhood in Sri Serdang, Selangor, Malaysia |
| title_fullStr | Presence of vancomycin resistance among enterococcus isolates from stray cats in Universiti Putra Malaysia and selected neighbourhood in Sri Serdang, Selangor, Malaysia |
| title_full_unstemmed | Presence of vancomycin resistance among enterococcus isolates from stray cats in Universiti Putra Malaysia and selected neighbourhood in Sri Serdang, Selangor, Malaysia |
| title_short | Presence of vancomycin resistance among enterococcus isolates from stray cats in Universiti Putra Malaysia and selected neighbourhood in Sri Serdang, Selangor, Malaysia |
| title_sort | presence of vancomycin resistance among enterococcus isolates from stray cats in universiti putra malaysia and selected neighbourhood in sri serdang selangor malaysia |
| url | http://psasir.upm.edu.my/id/eprint/27543/1/Proceedings-2.pdf |
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