Quality of salvaged epididymal spermatozoa in local dogs

The quality and quantity of epididymal spermatozoa and their relationship to scrotal circumference and testicular weight for local dogs in Malaysia were successfully determined. The longevity of spermatozoa in an alternative storage medium was also determined. The salvage of epididymal spermatozoa w...

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Main Authors: Jacob, Telma Dora, Dhaliwal, Gurmeet Kaur, Yusoff, Rosnina, Lim, Suit Fun
Format: Conference or Workshop Item
Language:English
Published: 2010
Online Access:http://psasir.upm.edu.my/id/eprint/27657/1/Proceedings-16.pdf
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author Jacob, Telma Dora
Dhaliwal, Gurmeet Kaur
Yusoff, Rosnina
Lim, Suit Fun
author_facet Jacob, Telma Dora
Dhaliwal, Gurmeet Kaur
Yusoff, Rosnina
Lim, Suit Fun
author_sort Jacob, Telma Dora
collection UPM
description The quality and quantity of epididymal spermatozoa and their relationship to scrotal circumference and testicular weight for local dogs in Malaysia were successfully determined. The longevity of spermatozoa in an alternative storage medium was also determined. The salvage of epididymal spermatozoa was done in twenty-two humanely euthanised adult local dogs. The overall mean ± SEM of the total number, general motility, progressive motility, and percentage live and abnormal spermatozoa of cauda epididymal spermatozoa salvaged per dog were 408 ± 55.31 x 106, 80.91 ± 1.84%, 46.25 ± 3.34%, 72.43 ± 1.47% and 6.80 ± 0.95%, respectively. The scrotal circumference and testicular weight were not appropriate indicators of the number of salvageable cauda epididymal spermatozoa as indicated by moderate correlation coefficients of 0.545 and 0.546, respectively. There was a significant difference (P<0.05) in general motility, progressive motility and live percentage of spermatozoa during storage (4-8ºC) between an extender based on 0.9% NaCl and a commercial canine semen extender over time. However, for the first 2 days, these parameters were similar in value. Therefore, 0.9% NaCl should be further investigated as a potential medium for short term storage of epididymal spermatozoa.
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spelling upm.eprints-276572015-01-08T10:23:29Z http://psasir.upm.edu.my/id/eprint/27657/ Quality of salvaged epididymal spermatozoa in local dogs Jacob, Telma Dora Dhaliwal, Gurmeet Kaur Yusoff, Rosnina Lim, Suit Fun The quality and quantity of epididymal spermatozoa and their relationship to scrotal circumference and testicular weight for local dogs in Malaysia were successfully determined. The longevity of spermatozoa in an alternative storage medium was also determined. The salvage of epididymal spermatozoa was done in twenty-two humanely euthanised adult local dogs. The overall mean ± SEM of the total number, general motility, progressive motility, and percentage live and abnormal spermatozoa of cauda epididymal spermatozoa salvaged per dog were 408 ± 55.31 x 106, 80.91 ± 1.84%, 46.25 ± 3.34%, 72.43 ± 1.47% and 6.80 ± 0.95%, respectively. The scrotal circumference and testicular weight were not appropriate indicators of the number of salvageable cauda epididymal spermatozoa as indicated by moderate correlation coefficients of 0.545 and 0.546, respectively. There was a significant difference (P<0.05) in general motility, progressive motility and live percentage of spermatozoa during storage (4-8ºC) between an extender based on 0.9% NaCl and a commercial canine semen extender over time. However, for the first 2 days, these parameters were similar in value. Therefore, 0.9% NaCl should be further investigated as a potential medium for short term storage of epididymal spermatozoa. 2010-01-05 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/27657/1/Proceedings-16.pdf Jacob, Telma Dora and Dhaliwal, Gurmeet Kaur and Yusoff, Rosnina and Lim, Suit Fun (2010) Quality of salvaged epididymal spermatozoa in local dogs. In: 5th Seminar on Veterinary Sciences, 5-8 Jan. 2010, Serdang, Selangor. .
spellingShingle Jacob, Telma Dora
Dhaliwal, Gurmeet Kaur
Yusoff, Rosnina
Lim, Suit Fun
Quality of salvaged epididymal spermatozoa in local dogs
title Quality of salvaged epididymal spermatozoa in local dogs
title_full Quality of salvaged epididymal spermatozoa in local dogs
title_fullStr Quality of salvaged epididymal spermatozoa in local dogs
title_full_unstemmed Quality of salvaged epididymal spermatozoa in local dogs
title_short Quality of salvaged epididymal spermatozoa in local dogs
title_sort quality of salvaged epididymal spermatozoa in local dogs
url http://psasir.upm.edu.my/id/eprint/27657/1/Proceedings-16.pdf
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