Statistical optimization of enzymatic degradation process for oil palm empty fruit bunch (OPEFB) in rotary drum bioreactor using crude cellulase produced from Aspergillus niger EFB1
Oil palm empty fruit bunch (OPEFB) was pretreated with 2% (v/v) HNO3 and degraded by Aspergillus niger EFB1 crude cellulase. Through 2 Level Factorial Design (2LFD), it was found that OPEFB concentration, temperature, incubation time, concentration of Tween 80 and agitation speed have significant ef...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/28032/1/28032.pdf |
| _version_ | 1796971248193896448 |
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| author | K., Noratiqah Md Salleh, Madihah B., Siti Aisyah Mohamad, Shaza Eva Abd. Aziz, Suraini Kamaruddin, Kamarulzaman |
| author_facet | K., Noratiqah Md Salleh, Madihah B., Siti Aisyah Mohamad, Shaza Eva Abd. Aziz, Suraini Kamaruddin, Kamarulzaman |
| author_sort | K., Noratiqah |
| collection | UPM |
| description | Oil palm empty fruit bunch (OPEFB) was pretreated with 2% (v/v) HNO3 and degraded by Aspergillus niger EFB1 crude cellulase. Through 2 Level Factorial Design (2LFD), it was found that OPEFB concentration, temperature, incubation time, concentration of Tween 80 and agitation speed have significant effect in reducing sugar production. A standard Response Surface Methodology (RSM) design known as Central Composite Design (CCD) was used to optimize the enzymatic degradation condition of OPEFB in rotary drum bioreactor. Reducing sugar level of 1.183 g/L was obtained with the following optimized degradation conditions: 1.95% (w/v) OPEFB, 0.5% (v/v) Tween 80, 55 °C, 87.5 rpm in the incubation period of 3 days and 16 h. The optimal degradation condition improved reducing sugar production by 1.07 fold compared to that before optimization in shake flasks culture. The optimization strategy of enzymatic degradation of OPEFB inside rotary drum bioreactor led to increase in glucose, xylose, arabinose, galactose and mannose production by 3, 2.5, 1.64, 19.37 and 22.52 fold, respectively. The improvement in reducing sugar and polyoses production were comparable with the reduction in OPEFB cellulose and hemicellulose content by 89.32% and 48.17% respectively after enzymatic degradation in optimized condition. |
| first_indexed | 2024-03-06T08:10:09Z |
| format | Article |
| id | upm.eprints-28032 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:10:09Z |
| publishDate | 2013 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-280322016-03-07T07:47:12Z http://psasir.upm.edu.my/id/eprint/28032/ Statistical optimization of enzymatic degradation process for oil palm empty fruit bunch (OPEFB) in rotary drum bioreactor using crude cellulase produced from Aspergillus niger EFB1 K., Noratiqah Md Salleh, Madihah B., Siti Aisyah Mohamad, Shaza Eva Abd. Aziz, Suraini Kamaruddin, Kamarulzaman Oil palm empty fruit bunch (OPEFB) was pretreated with 2% (v/v) HNO3 and degraded by Aspergillus niger EFB1 crude cellulase. Through 2 Level Factorial Design (2LFD), it was found that OPEFB concentration, temperature, incubation time, concentration of Tween 80 and agitation speed have significant effect in reducing sugar production. A standard Response Surface Methodology (RSM) design known as Central Composite Design (CCD) was used to optimize the enzymatic degradation condition of OPEFB in rotary drum bioreactor. Reducing sugar level of 1.183 g/L was obtained with the following optimized degradation conditions: 1.95% (w/v) OPEFB, 0.5% (v/v) Tween 80, 55 °C, 87.5 rpm in the incubation period of 3 days and 16 h. The optimal degradation condition improved reducing sugar production by 1.07 fold compared to that before optimization in shake flasks culture. The optimization strategy of enzymatic degradation of OPEFB inside rotary drum bioreactor led to increase in glucose, xylose, arabinose, galactose and mannose production by 3, 2.5, 1.64, 19.37 and 22.52 fold, respectively. The improvement in reducing sugar and polyoses production were comparable with the reduction in OPEFB cellulose and hemicellulose content by 89.32% and 48.17% respectively after enzymatic degradation in optimized condition. Elsevier 2013-06-15 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28032/1/28032.pdf K., Noratiqah and Md Salleh, Madihah and B., Siti Aisyah and Mohamad, Shaza Eva and Abd. Aziz, Suraini and Kamaruddin, Kamarulzaman (2013) Statistical optimization of enzymatic degradation process for oil palm empty fruit bunch (OPEFB) in rotary drum bioreactor using crude cellulase produced from Aspergillus niger EFB1. Biochemical Engineering Journal, 75. pp. 8-20. ISSN 1369-703X; ESSN: 1873-295X 10.1016/j.bej.2013.03.007 |
| spellingShingle | K., Noratiqah Md Salleh, Madihah B., Siti Aisyah Mohamad, Shaza Eva Abd. Aziz, Suraini Kamaruddin, Kamarulzaman Statistical optimization of enzymatic degradation process for oil palm empty fruit bunch (OPEFB) in rotary drum bioreactor using crude cellulase produced from Aspergillus niger EFB1 |
| title | Statistical optimization of enzymatic degradation process for oil palm empty fruit bunch (OPEFB) in rotary drum bioreactor using crude cellulase produced from Aspergillus niger EFB1 |
| title_full | Statistical optimization of enzymatic degradation process for oil palm empty fruit bunch (OPEFB) in rotary drum bioreactor using crude cellulase produced from Aspergillus niger EFB1 |
| title_fullStr | Statistical optimization of enzymatic degradation process for oil palm empty fruit bunch (OPEFB) in rotary drum bioreactor using crude cellulase produced from Aspergillus niger EFB1 |
| title_full_unstemmed | Statistical optimization of enzymatic degradation process for oil palm empty fruit bunch (OPEFB) in rotary drum bioreactor using crude cellulase produced from Aspergillus niger EFB1 |
| title_short | Statistical optimization of enzymatic degradation process for oil palm empty fruit bunch (OPEFB) in rotary drum bioreactor using crude cellulase produced from Aspergillus niger EFB1 |
| title_sort | statistical optimization of enzymatic degradation process for oil palm empty fruit bunch opefb in rotary drum bioreactor using crude cellulase produced from aspergillus niger efb1 |
| url | http://psasir.upm.edu.my/id/eprint/28032/1/28032.pdf |
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