Effect of C/N ratio and media optimization through response surface methodology on simultaneous productions of intra- and extracellular inulinase and invertase from Aspergillus niger ATCC 20611
The study is to identify the extraction of intracellular inulinase (exo- and endoinulinase) and invertase as well as optimization medium composition for maximum productions of intra- and extracellular enzymes from Aspergillus niger ATCC 20611. From two different methods for extraction of intracellu...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/28054/1/508968.pdf |
| _version_ | 1796971252761493504 |
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| author | Dinarvand, Mojdeh Rezaee, Malahat Masomian, Malihe Jazayeri, Seyed Davoud Zareian, Mohsen Abbasiliasi, Sahar Ariff, Arbakariya |
| author_facet | Dinarvand, Mojdeh Rezaee, Malahat Masomian, Malihe Jazayeri, Seyed Davoud Zareian, Mohsen Abbasiliasi, Sahar Ariff, Arbakariya |
| author_sort | Dinarvand, Mojdeh |
| collection | UPM |
| description | The study is to identify the extraction of intracellular inulinase (exo- and endoinulinase) and invertase as well as optimization medium composition for maximum productions of intra- and extracellular enzymes from Aspergillus niger ATCC 20611. From two different methods for extraction of intracellular enzymes, ultrasonic method was found more effective. Response surface methodology (RSM) with a five-variable and three-level central composite design (CCD) was employed to optimize the medium composition. The effect of five main reaction parameters including sucrose, yeast extract, NaNO3, Zn+2, and Triton X-100 on the production of enzymes was analyzed. A modified quadratic model was fitted to the data with a coefficient of determination (R2) more than 0.90 for all responses. The intra-extracellular inulinase and invertase productions increased in the range from 16 to 8.4 times in the optimized medium (10% (w/v) sucrose, 2.5% (w/v) yeast extract, 2% (w/v) NaNO3, 1.5 mM (v/v) Zn+2, and 1% (v/v) Triton X-100) by RSM and from around 1.2 to 1.3 times greater than in the medium optimized by one-factor-at-a-time, respectively. The results of bioprocesses optimization can be useful in the scale-up fermentation and food industry. |
| first_indexed | 2024-03-06T08:10:13Z |
| format | Article |
| id | upm.eprints-28054 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:10:13Z |
| publishDate | 2013 |
| publisher | Hindawi Publishing Corporation |
| record_format | dspace |
| spelling | upm.eprints-280542016-04-22T03:28:14Z http://psasir.upm.edu.my/id/eprint/28054/ Effect of C/N ratio and media optimization through response surface methodology on simultaneous productions of intra- and extracellular inulinase and invertase from Aspergillus niger ATCC 20611 Dinarvand, Mojdeh Rezaee, Malahat Masomian, Malihe Jazayeri, Seyed Davoud Zareian, Mohsen Abbasiliasi, Sahar Ariff, Arbakariya The study is to identify the extraction of intracellular inulinase (exo- and endoinulinase) and invertase as well as optimization medium composition for maximum productions of intra- and extracellular enzymes from Aspergillus niger ATCC 20611. From two different methods for extraction of intracellular enzymes, ultrasonic method was found more effective. Response surface methodology (RSM) with a five-variable and three-level central composite design (CCD) was employed to optimize the medium composition. The effect of five main reaction parameters including sucrose, yeast extract, NaNO3, Zn+2, and Triton X-100 on the production of enzymes was analyzed. A modified quadratic model was fitted to the data with a coefficient of determination (R2) more than 0.90 for all responses. The intra-extracellular inulinase and invertase productions increased in the range from 16 to 8.4 times in the optimized medium (10% (w/v) sucrose, 2.5% (w/v) yeast extract, 2% (w/v) NaNO3, 1.5 mM (v/v) Zn+2, and 1% (v/v) Triton X-100) by RSM and from around 1.2 to 1.3 times greater than in the medium optimized by one-factor-at-a-time, respectively. The results of bioprocesses optimization can be useful in the scale-up fermentation and food industry. Hindawi Publishing Corporation 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28054/1/508968.pdf Dinarvand, Mojdeh and Rezaee, Malahat and Masomian, Malihe and Jazayeri, Seyed Davoud and Zareian, Mohsen and Abbasiliasi, Sahar and Ariff, Arbakariya (2013) Effect of C/N ratio and media optimization through response surface methodology on simultaneous productions of intra- and extracellular inulinase and invertase from Aspergillus niger ATCC 20611. BioMed Research International, 2013. art. no. 508968. pp. 1-13. ISSN 2314-6133; ESSN: 2314-6141 http://www.hindawi.com/journals/bmri/2013/508968/abs/ 10.1155/2013/508968 |
| spellingShingle | Dinarvand, Mojdeh Rezaee, Malahat Masomian, Malihe Jazayeri, Seyed Davoud Zareian, Mohsen Abbasiliasi, Sahar Ariff, Arbakariya Effect of C/N ratio and media optimization through response surface methodology on simultaneous productions of intra- and extracellular inulinase and invertase from Aspergillus niger ATCC 20611 |
| title | Effect of C/N ratio and media optimization through response surface methodology on simultaneous productions of intra- and extracellular inulinase and invertase from Aspergillus niger ATCC 20611 |
| title_full | Effect of C/N ratio and media optimization through response surface methodology on simultaneous productions of intra- and extracellular inulinase and invertase from Aspergillus niger ATCC 20611 |
| title_fullStr | Effect of C/N ratio and media optimization through response surface methodology on simultaneous productions of intra- and extracellular inulinase and invertase from Aspergillus niger ATCC 20611 |
| title_full_unstemmed | Effect of C/N ratio and media optimization through response surface methodology on simultaneous productions of intra- and extracellular inulinase and invertase from Aspergillus niger ATCC 20611 |
| title_short | Effect of C/N ratio and media optimization through response surface methodology on simultaneous productions of intra- and extracellular inulinase and invertase from Aspergillus niger ATCC 20611 |
| title_sort | effect of c n ratio and media optimization through response surface methodology on simultaneous productions of intra and extracellular inulinase and invertase from aspergillus niger atcc 20611 |
| url | http://psasir.upm.edu.my/id/eprint/28054/1/508968.pdf |
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