An integrated bioreactor-expanded bed adsorption system for the removal of acetate to enhance the production of alpha-interferon-2b by Escherichia coli
A stirred tank bioreactor (STB) integrated with an expanded bed adsorption (EBA) system containing anion-exchange resin (Diaion WA30) was developed for in situ removal of acetate to increase the production of α-interferon-2b (α-PrIFN-2b) by Escherichia coli (E. coli). Although the total acetate (9.7...
| Principais autores: | , , , , , |
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| Formato: | Artigo |
| Idioma: | English |
| Publicado em: |
Elsevier
2013
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| Acesso em linha: | http://psasir.upm.edu.my/id/eprint/28066/1/An%20integrated%20bioreactor-expanded%20bed%20adsorption%20system%20for%20the%20removal%20of%20acetate%20to%20enhance%20the%20production%20of%20alpha-interferon-2b%20by%20Escherichia%20coli.pdf |
| _version_ | 1825947243532779520 |
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| author | Tan, Joo Shun Ling, Tau Chuan Mustafa, Shuhaimi Tam, Yew Joon Ramanan, Ramakrishnan Nagasundara Ariff, Arbakariya |
| author_facet | Tan, Joo Shun Ling, Tau Chuan Mustafa, Shuhaimi Tam, Yew Joon Ramanan, Ramakrishnan Nagasundara Ariff, Arbakariya |
| author_sort | Tan, Joo Shun |
| collection | UPM |
| description | A stirred tank bioreactor (STB) integrated with an expanded bed adsorption (EBA) system containing anion-exchange resin (Diaion WA30) was developed for in situ removal of acetate to increase the production of α-interferon-2b (α-PrIFN-2b) by Escherichia coli (E. coli). Although the total acetate (9.79 g/L) secreted by E. coli in the integrated STB/EBA system was higher than that in a bioreactor with dispersed resin or a conventional batch bioreactor, cell growth (14.97 g/L) and α-PrIFN-2b production (867.4 μg/L) were significantly improved owing to the high efficiency of acetate removal from the culture. The production of α-PrIFN-2b in the integrated STB/EBA system was improved by 3-fold and 1.4-fold over that obtained in a conventional batch bioreactor and a bioreactor containing dispersed resins, respectively. |
| first_indexed | 2024-03-06T08:10:15Z |
| format | Article |
| id | upm.eprints-28066 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:10:15Z |
| publishDate | 2013 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-280662016-04-21T06:08:18Z http://psasir.upm.edu.my/id/eprint/28066/ An integrated bioreactor-expanded bed adsorption system for the removal of acetate to enhance the production of alpha-interferon-2b by Escherichia coli Tan, Joo Shun Ling, Tau Chuan Mustafa, Shuhaimi Tam, Yew Joon Ramanan, Ramakrishnan Nagasundara Ariff, Arbakariya A stirred tank bioreactor (STB) integrated with an expanded bed adsorption (EBA) system containing anion-exchange resin (Diaion WA30) was developed for in situ removal of acetate to increase the production of α-interferon-2b (α-PrIFN-2b) by Escherichia coli (E. coli). Although the total acetate (9.79 g/L) secreted by E. coli in the integrated STB/EBA system was higher than that in a bioreactor with dispersed resin or a conventional batch bioreactor, cell growth (14.97 g/L) and α-PrIFN-2b production (867.4 μg/L) were significantly improved owing to the high efficiency of acetate removal from the culture. The production of α-PrIFN-2b in the integrated STB/EBA system was improved by 3-fold and 1.4-fold over that obtained in a conventional batch bioreactor and a bioreactor containing dispersed resins, respectively. Elsevier 2013-04 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28066/1/An%20integrated%20bioreactor-expanded%20bed%20adsorption%20system%20for%20the%20removal%20of%20acetate%20to%20enhance%20the%20production%20of%20alpha-interferon-2b%20by%20Escherichia%20coli.pdf Tan, Joo Shun and Ling, Tau Chuan and Mustafa, Shuhaimi and Tam, Yew Joon and Ramanan, Ramakrishnan Nagasundara and Ariff, Arbakariya (2013) An integrated bioreactor-expanded bed adsorption system for the removal of acetate to enhance the production of alpha-interferon-2b by Escherichia coli. Process Biochemistry, 48 (4). pp. 551-558. ISSN 1359-5113; ESSN: 1873-3298 10.1016/j.procbio.2013.02.024 |
| spellingShingle | Tan, Joo Shun Ling, Tau Chuan Mustafa, Shuhaimi Tam, Yew Joon Ramanan, Ramakrishnan Nagasundara Ariff, Arbakariya An integrated bioreactor-expanded bed adsorption system for the removal of acetate to enhance the production of alpha-interferon-2b by Escherichia coli |
| title | An integrated bioreactor-expanded bed adsorption system for the removal of acetate to enhance the production of alpha-interferon-2b by Escherichia coli |
| title_full | An integrated bioreactor-expanded bed adsorption system for the removal of acetate to enhance the production of alpha-interferon-2b by Escherichia coli |
| title_fullStr | An integrated bioreactor-expanded bed adsorption system for the removal of acetate to enhance the production of alpha-interferon-2b by Escherichia coli |
| title_full_unstemmed | An integrated bioreactor-expanded bed adsorption system for the removal of acetate to enhance the production of alpha-interferon-2b by Escherichia coli |
| title_short | An integrated bioreactor-expanded bed adsorption system for the removal of acetate to enhance the production of alpha-interferon-2b by Escherichia coli |
| title_sort | integrated bioreactor expanded bed adsorption system for the removal of acetate to enhance the production of alpha interferon 2b by escherichia coli |
| url | http://psasir.upm.edu.my/id/eprint/28066/1/An%20integrated%20bioreactor-expanded%20bed%20adsorption%20system%20for%20the%20removal%20of%20acetate%20to%20enhance%20the%20production%20of%20alpha-interferon-2b%20by%20Escherichia%20coli.pdf |
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