Improved properties of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Comamonas sp. EB172 utilizing volatile fatty acids by regulating the nitrogen source
This study presents the effect of carbon to nitrogen ratio (C/N) (mol/mol) on the cell growth and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) accumulation by Comamonas sp. EB172 in 2 L fermenters using volatile fatty acids (VFA) as the carbon source. This VFA was supplemented with ammonium sulphate...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Hindawi Publishing Corporation
2013
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/28068/1/28068.pdf |
| _version_ | 1825947243923898368 |
|---|---|
| author | Zakaria @ Mamat, Mohd Rafein Ariffin, Hidayah Abd. Aziz, Suraini Hassan, Mohd Ali Shirai, Yoshihito |
| author_facet | Zakaria @ Mamat, Mohd Rafein Ariffin, Hidayah Abd. Aziz, Suraini Hassan, Mohd Ali Shirai, Yoshihito |
| author_sort | Zakaria @ Mamat, Mohd Rafein |
| collection | UPM |
| description | This study presents the effect of carbon to nitrogen ratio (C/N) (mol/mol) on the cell growth and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) accumulation by Comamonas sp. EB172 in 2 L fermenters using volatile fatty acids (VFA) as the carbon source. This VFA was supplemented with ammonium sulphate and yeast extract in the feeding solution to achieve C/N (mol/mol) 5, 15, 25, and 34.4, respectively. By extrapolating the C/N and the source of nitrogen, the properties of the polymers can be regulated. The number average molecular weight (M n) of P(3HB-co-3HV) copolymer reached the highest at 838 × 10 3 Da with polydispersity index (PDI) value of 1.8, when the culture broth was supplemented with yeast extract (C/N 34.4). Tensile strength and Young's modulus of the copolymer containing 6-8 mol% 3HV were in the ranges of 13-14.4 MPa and 0.26-0.34 GPa, respectively, comparable to those of polyethylene (PE). Thus, Comamonas sp. EB172 has shown promising bacterial isolates producing polyhydroxyalkanoates from renewable carbon materials. |
| first_indexed | 2024-03-06T08:10:15Z |
| format | Article |
| id | upm.eprints-28068 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:10:15Z |
| publishDate | 2013 |
| publisher | Hindawi Publishing Corporation |
| record_format | dspace |
| spelling | upm.eprints-280682016-06-20T03:06:25Z http://psasir.upm.edu.my/id/eprint/28068/ Improved properties of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Comamonas sp. EB172 utilizing volatile fatty acids by regulating the nitrogen source Zakaria @ Mamat, Mohd Rafein Ariffin, Hidayah Abd. Aziz, Suraini Hassan, Mohd Ali Shirai, Yoshihito This study presents the effect of carbon to nitrogen ratio (C/N) (mol/mol) on the cell growth and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) accumulation by Comamonas sp. EB172 in 2 L fermenters using volatile fatty acids (VFA) as the carbon source. This VFA was supplemented with ammonium sulphate and yeast extract in the feeding solution to achieve C/N (mol/mol) 5, 15, 25, and 34.4, respectively. By extrapolating the C/N and the source of nitrogen, the properties of the polymers can be regulated. The number average molecular weight (M n) of P(3HB-co-3HV) copolymer reached the highest at 838 × 10 3 Da with polydispersity index (PDI) value of 1.8, when the culture broth was supplemented with yeast extract (C/N 34.4). Tensile strength and Young's modulus of the copolymer containing 6-8 mol% 3HV were in the ranges of 13-14.4 MPa and 0.26-0.34 GPa, respectively, comparable to those of polyethylene (PE). Thus, Comamonas sp. EB172 has shown promising bacterial isolates producing polyhydroxyalkanoates from renewable carbon materials. Hindawi Publishing Corporation 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28068/1/28068.pdf Zakaria @ Mamat, Mohd Rafein and Ariffin, Hidayah and Abd. Aziz, Suraini and Hassan, Mohd Ali and Shirai, Yoshihito (2013) Improved properties of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Comamonas sp. EB172 utilizing volatile fatty acids by regulating the nitrogen source. BioMed Research International, 2013. art. no. 237806. pp. 1-7. ISSN 2314-6133; ESSN: 2314-6141 http://www.hindawi.com/journals/bmri/2013/237806/abs/ 10.1155/2013/237806 |
| spellingShingle | Zakaria @ Mamat, Mohd Rafein Ariffin, Hidayah Abd. Aziz, Suraini Hassan, Mohd Ali Shirai, Yoshihito Improved properties of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Comamonas sp. EB172 utilizing volatile fatty acids by regulating the nitrogen source |
| title | Improved properties of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Comamonas sp. EB172 utilizing volatile fatty acids by regulating the nitrogen source |
| title_full | Improved properties of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Comamonas sp. EB172 utilizing volatile fatty acids by regulating the nitrogen source |
| title_fullStr | Improved properties of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Comamonas sp. EB172 utilizing volatile fatty acids by regulating the nitrogen source |
| title_full_unstemmed | Improved properties of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Comamonas sp. EB172 utilizing volatile fatty acids by regulating the nitrogen source |
| title_short | Improved properties of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Comamonas sp. EB172 utilizing volatile fatty acids by regulating the nitrogen source |
| title_sort | improved properties of poly 3 hydroxybutyrate co 3 hydroxyvalerate produced by comamonas sp eb172 utilizing volatile fatty acids by regulating the nitrogen source |
| url | http://psasir.upm.edu.my/id/eprint/28068/1/28068.pdf |
| work_keys_str_mv | AT zakariamamatmohdrafein improvedpropertiesofpoly3hydroxybutyrateco3hydroxyvalerateproducedbycomamonasspeb172utilizingvolatilefattyacidsbyregulatingthenitrogensource AT ariffinhidayah improvedpropertiesofpoly3hydroxybutyrateco3hydroxyvalerateproducedbycomamonasspeb172utilizingvolatilefattyacidsbyregulatingthenitrogensource AT abdazizsuraini improvedpropertiesofpoly3hydroxybutyrateco3hydroxyvalerateproducedbycomamonasspeb172utilizingvolatilefattyacidsbyregulatingthenitrogensource AT hassanmohdali improvedpropertiesofpoly3hydroxybutyrateco3hydroxyvalerateproducedbycomamonasspeb172utilizingvolatilefattyacidsbyregulatingthenitrogensource AT shiraiyoshihito improvedpropertiesofpoly3hydroxybutyrateco3hydroxyvalerateproducedbycomamonasspeb172utilizingvolatilefattyacidsbyregulatingthenitrogensource |