Extractive bioconversion of cyclodextrins by Bacillus cereus cyclodextrin glycosyltransferase in aqueous two-phase system
An extractive bioconversion with Bacillus cereus cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) in aqueous two-phase system (ATPS) was investigated for the synthesis and recovery of cyclodextrins (CDs). Optimum condition for the extractive bioconversion of CDs was achieved in ATPS consisted...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/28078/1/28078.pdf |
| _version_ | 1825947245871104000 |
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| author | Ng, Hui Suan Ooi, Chien Wei Mokhtar, Mohd Noriznan Show, Pau Loke Ariff, Arbakariya Tan, Joo Shun Ng, Eng Poh Ling, Tau Chuan |
| author_facet | Ng, Hui Suan Ooi, Chien Wei Mokhtar, Mohd Noriznan Show, Pau Loke Ariff, Arbakariya Tan, Joo Shun Ng, Eng Poh Ling, Tau Chuan |
| author_sort | Ng, Hui Suan |
| collection | UPM |
| description | An extractive bioconversion with Bacillus cereus cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) in aqueous two-phase system (ATPS) was investigated for the synthesis and recovery of cyclodextrins (CDs). Optimum condition for the extractive bioconversion of CDs was achieved in ATPS consisted of 7.7% (w/w) polyethylene glycol (PEG) 20,000 and 10.3% (w/w) dextran T500 with volume ratio (VR) of 4.0. Enzymatic conversion of starch occurred mainly in dextran-rich bottom phase whereas the product, CDs was transferred to top phase and a higher partition coefficient of CDs was achieved. Repetitive batch of CDs synthesis was employed by replenishment of the top phase components and addition of starch every 8 h. An average total CDs concentration of 13.7 mg/mL, (4.77 mg/mLα-CD, 5.02 mg/mLβ-CD and 3.91 mg/mLγ-CD) was recovered in the top phase of PEG 20,000/dextran T500 ATPS. This study showed the effectiveness of ATPS application in extractive bioconversion of CDs synthesis with B. cereus CGTase. |
| first_indexed | 2024-03-06T08:10:17Z |
| format | Article |
| id | upm.eprints-28078 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:10:17Z |
| publishDate | 2013 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-280782016-04-21T09:37:17Z http://psasir.upm.edu.my/id/eprint/28078/ Extractive bioconversion of cyclodextrins by Bacillus cereus cyclodextrin glycosyltransferase in aqueous two-phase system Ng, Hui Suan Ooi, Chien Wei Mokhtar, Mohd Noriznan Show, Pau Loke Ariff, Arbakariya Tan, Joo Shun Ng, Eng Poh Ling, Tau Chuan An extractive bioconversion with Bacillus cereus cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) in aqueous two-phase system (ATPS) was investigated for the synthesis and recovery of cyclodextrins (CDs). Optimum condition for the extractive bioconversion of CDs was achieved in ATPS consisted of 7.7% (w/w) polyethylene glycol (PEG) 20,000 and 10.3% (w/w) dextran T500 with volume ratio (VR) of 4.0. Enzymatic conversion of starch occurred mainly in dextran-rich bottom phase whereas the product, CDs was transferred to top phase and a higher partition coefficient of CDs was achieved. Repetitive batch of CDs synthesis was employed by replenishment of the top phase components and addition of starch every 8 h. An average total CDs concentration of 13.7 mg/mL, (4.77 mg/mLα-CD, 5.02 mg/mLβ-CD and 3.91 mg/mLγ-CD) was recovered in the top phase of PEG 20,000/dextran T500 ATPS. This study showed the effectiveness of ATPS application in extractive bioconversion of CDs synthesis with B. cereus CGTase. Elsevier 2013-08 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28078/1/28078.pdf Ng, Hui Suan and Ooi, Chien Wei and Mokhtar, Mohd Noriznan and Show, Pau Loke and Ariff, Arbakariya and Tan, Joo Shun and Ng, Eng Poh and Ling, Tau Chuan (2013) Extractive bioconversion of cyclodextrins by Bacillus cereus cyclodextrin glycosyltransferase in aqueous two-phase system. Bioresource Technology, 142. pp. 723-726. ISSN 0960-8524; ESSN: 1873-2976 http://www.elsevier.com/locate/biortech 10.1016/j.biortech.2013.05.087 |
| spellingShingle | Ng, Hui Suan Ooi, Chien Wei Mokhtar, Mohd Noriznan Show, Pau Loke Ariff, Arbakariya Tan, Joo Shun Ng, Eng Poh Ling, Tau Chuan Extractive bioconversion of cyclodextrins by Bacillus cereus cyclodextrin glycosyltransferase in aqueous two-phase system |
| title | Extractive bioconversion of cyclodextrins by Bacillus cereus cyclodextrin glycosyltransferase in aqueous two-phase system |
| title_full | Extractive bioconversion of cyclodextrins by Bacillus cereus cyclodextrin glycosyltransferase in aqueous two-phase system |
| title_fullStr | Extractive bioconversion of cyclodextrins by Bacillus cereus cyclodextrin glycosyltransferase in aqueous two-phase system |
| title_full_unstemmed | Extractive bioconversion of cyclodextrins by Bacillus cereus cyclodextrin glycosyltransferase in aqueous two-phase system |
| title_short | Extractive bioconversion of cyclodextrins by Bacillus cereus cyclodextrin glycosyltransferase in aqueous two-phase system |
| title_sort | extractive bioconversion of cyclodextrins by bacillus cereus cyclodextrin glycosyltransferase in aqueous two phase system |
| url | http://psasir.upm.edu.my/id/eprint/28078/1/28078.pdf |
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