Relationship between total carbon, total nitrogen and carbon to nitrogen ratio on growth, sporulation rate and δ-endotoxin synthesis of Bacillus thuringiensis.
The relationship between intracellular and extracellular total carbon, total nitrogen and carbon to nitrogen ratio on Bacillus thuringiensis MPK13 growth, sporulation rate and d-endotoxin synthesis were carried out in shake flask using different types of carbon (glucose, galactose, sucrose, lactose...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English English |
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Edizioni Minerva Medica
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/28110/1/Relationship%20between%20total%20carbon.pdf |
| _version_ | 1796971264480378880 |
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| author | Mohd Masri, Mohamed Mazmira Ahmad Ali, Siti Ramlah Mohamad, Rosfarizan Ling, T. C. Ariff, Arbakariya |
| author_facet | Mohd Masri, Mohamed Mazmira Ahmad Ali, Siti Ramlah Mohamad, Rosfarizan Ling, T. C. Ariff, Arbakariya |
| author_sort | Mohd Masri, Mohamed Mazmira |
| collection | UPM |
| description | The relationship between intracellular and extracellular total carbon, total nitrogen and carbon to nitrogen ratio on Bacillus thuringiensis MPK13 growth, sporulation rate and d-endotoxin synthesis were carried out in shake flask using different types of carbon (glucose, galactose, sucrose, lactose and maltose) and nitrogen (yeast extract) sources. The highest initial intracellular TC (5.15 g/L) and intracellular TN (1.45 g/L) were recorded in medium containing glucose. On the other hand, the highest initial extracellular TC (34.54 g/L) and TN (7.61 g/L) were recorded in medium containing maltose and lactose, respectively. At 48 h of cultivation, the highest final intracellular TC (11.96 g/L) and intracellular TN (3.26 g/L) were also recorded in medium containing glucose. The highest final extracellular TC (27.85 g/L) and TN (7.27 g/L) were recorded in medium containing galactose which showed the lowest growth and sporulation. The presence of d-endotoxin was only detected at 48 h of cultivation using medium containing glucose. The result indicated that high initial intracellular TC and TN values during the cultivation anticipated in high cell growth, sporulation and d-endotoxin production and may be used for indirect measurement of cultivation performance for B. thuringiensis. |
| first_indexed | 2024-03-06T08:10:23Z |
| format | Article |
| id | upm.eprints-28110 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T08:10:23Z |
| publishDate | 2013 |
| publisher | Edizioni Minerva Medica |
| record_format | dspace |
| spelling | upm.eprints-281102015-10-08T07:30:07Z http://psasir.upm.edu.my/id/eprint/28110/ Relationship between total carbon, total nitrogen and carbon to nitrogen ratio on growth, sporulation rate and δ-endotoxin synthesis of Bacillus thuringiensis. Mohd Masri, Mohamed Mazmira Ahmad Ali, Siti Ramlah Mohamad, Rosfarizan Ling, T. C. Ariff, Arbakariya The relationship between intracellular and extracellular total carbon, total nitrogen and carbon to nitrogen ratio on Bacillus thuringiensis MPK13 growth, sporulation rate and d-endotoxin synthesis were carried out in shake flask using different types of carbon (glucose, galactose, sucrose, lactose and maltose) and nitrogen (yeast extract) sources. The highest initial intracellular TC (5.15 g/L) and intracellular TN (1.45 g/L) were recorded in medium containing glucose. On the other hand, the highest initial extracellular TC (34.54 g/L) and TN (7.61 g/L) were recorded in medium containing maltose and lactose, respectively. At 48 h of cultivation, the highest final intracellular TC (11.96 g/L) and intracellular TN (3.26 g/L) were also recorded in medium containing glucose. The highest final extracellular TC (27.85 g/L) and TN (7.27 g/L) were recorded in medium containing galactose which showed the lowest growth and sporulation. The presence of d-endotoxin was only detected at 48 h of cultivation using medium containing glucose. The result indicated that high initial intracellular TC and TN values during the cultivation anticipated in high cell growth, sporulation and d-endotoxin production and may be used for indirect measurement of cultivation performance for B. thuringiensis. Edizioni Minerva Medica 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28110/1/Relationship%20between%20total%20carbon.pdf Mohd Masri, Mohamed Mazmira and Ahmad Ali, Siti Ramlah and Mohamad, Rosfarizan and Ling, T. C. and Ariff, Arbakariya (2013) Relationship between total carbon, total nitrogen and carbon to nitrogen ratio on growth, sporulation rate and δ-endotoxin synthesis of Bacillus thuringiensis. Minerva Biotecnologica, 25 (4). pp. 219-25. ISSN 1120-4826; EISSN: 1827-160X http://www.minervamedica.it/en/journals/minerva-biotecnologica/issue.php?cod=R04Y2013N04 English |
| spellingShingle | Mohd Masri, Mohamed Mazmira Ahmad Ali, Siti Ramlah Mohamad, Rosfarizan Ling, T. C. Ariff, Arbakariya Relationship between total carbon, total nitrogen and carbon to nitrogen ratio on growth, sporulation rate and δ-endotoxin synthesis of Bacillus thuringiensis. |
| title | Relationship between total carbon, total nitrogen and carbon to nitrogen ratio on growth, sporulation rate and δ-endotoxin synthesis of Bacillus thuringiensis. |
| title_full | Relationship between total carbon, total nitrogen and carbon to nitrogen ratio on growth, sporulation rate and δ-endotoxin synthesis of Bacillus thuringiensis. |
| title_fullStr | Relationship between total carbon, total nitrogen and carbon to nitrogen ratio on growth, sporulation rate and δ-endotoxin synthesis of Bacillus thuringiensis. |
| title_full_unstemmed | Relationship between total carbon, total nitrogen and carbon to nitrogen ratio on growth, sporulation rate and δ-endotoxin synthesis of Bacillus thuringiensis. |
| title_short | Relationship between total carbon, total nitrogen and carbon to nitrogen ratio on growth, sporulation rate and δ-endotoxin synthesis of Bacillus thuringiensis. |
| title_sort | relationship between total carbon total nitrogen and carbon to nitrogen ratio on growth sporulation rate and δ endotoxin synthesis of bacillus thuringiensis |
| url | http://psasir.upm.edu.my/id/eprint/28110/1/Relationship%20between%20total%20carbon.pdf |
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