Refinement of a vitrification protocol for protocorm-like bodies of Dendrobium sonia-28.

The hybrid Dendrobium sonia-28 is an important ornamental orchid in the Malaysian flower industry. However, it faces the risk of producing heterozygous progenies through conventional seed propagation or somaclonal variation. Cryopreservation is a favoured long-term storage method in the preserva...

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Main Authors: Poobathy, Ranjetta, Sinniah, Uma Rani, Mahmood, Maziah, Subramaniam, Sreeramanan
Format: Article
Language:English
Published: Scientific and Technical Research Council of Turkey 2013
Online Access:http://psasir.upm.edu.my/id/eprint/28115/1/Refinement%20of%20a%20vitrification%20protocol%20for%20protocorm.pdf
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author Poobathy, Ranjetta
Sinniah, Uma Rani
Mahmood, Maziah
Subramaniam, Sreeramanan
author_facet Poobathy, Ranjetta
Sinniah, Uma Rani
Mahmood, Maziah
Subramaniam, Sreeramanan
author_sort Poobathy, Ranjetta
collection UPM
description The hybrid Dendrobium sonia-28 is an important ornamental orchid in the Malaysian flower industry. However, it faces the risk of producing heterozygous progenies through conventional seed propagation or somaclonal variation. Cryopreservation is a favoured long-term storage method in the preservation and maintenance of orchids with specific desired traits. This paper presents a successful cryopreservation protocol for protocorm-like bodies (PLBs) of Dendrobium sonia-28 through the vitrification technique and the use of ascorbic acid and charcoal. Survival assessments were conducted through growth evaluations and visual and spectrophotometric 2,3,5-triphenyltetrazolium chloride assays. Results of this study, an improvement over the previous protocol applied to this orchid hybrid, showed that the addition of ascorbic acid to the preculture, loading, dehydration, unloading, and regeneration media, coupled with the addition of charcoal to the regeneration medium, helped boost recovery rates of cryopreserved PLBs of Dendrobium sonia-28.
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spelling upm.eprints-281152015-10-28T05:59:08Z http://psasir.upm.edu.my/id/eprint/28115/ Refinement of a vitrification protocol for protocorm-like bodies of Dendrobium sonia-28. Poobathy, Ranjetta Sinniah, Uma Rani Mahmood, Maziah Subramaniam, Sreeramanan The hybrid Dendrobium sonia-28 is an important ornamental orchid in the Malaysian flower industry. However, it faces the risk of producing heterozygous progenies through conventional seed propagation or somaclonal variation. Cryopreservation is a favoured long-term storage method in the preservation and maintenance of orchids with specific desired traits. This paper presents a successful cryopreservation protocol for protocorm-like bodies (PLBs) of Dendrobium sonia-28 through the vitrification technique and the use of ascorbic acid and charcoal. Survival assessments were conducted through growth evaluations and visual and spectrophotometric 2,3,5-triphenyltetrazolium chloride assays. Results of this study, an improvement over the previous protocol applied to this orchid hybrid, showed that the addition of ascorbic acid to the preculture, loading, dehydration, unloading, and regeneration media, coupled with the addition of charcoal to the regeneration medium, helped boost recovery rates of cryopreserved PLBs of Dendrobium sonia-28. Scientific and Technical Research Council of Turkey 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28115/1/Refinement%20of%20a%20vitrification%20protocol%20for%20protocorm.pdf Poobathy, Ranjetta and Sinniah, Uma Rani and Mahmood, Maziah and Subramaniam, Sreeramanan (2013) Refinement of a vitrification protocol for protocorm-like bodies of Dendrobium sonia-28. Turkish Journal of Botany, 37 (5). pp. 940-949. ISSN 1300-008X; ESSN: 1303-6106 http://mistug.tubitak.gov.tr/bdyim/toc.php?dergi=bot&yilsayi=2013/5 10.3906/bot-1208-35
spellingShingle Poobathy, Ranjetta
Sinniah, Uma Rani
Mahmood, Maziah
Subramaniam, Sreeramanan
Refinement of a vitrification protocol for protocorm-like bodies of Dendrobium sonia-28.
title Refinement of a vitrification protocol for protocorm-like bodies of Dendrobium sonia-28.
title_full Refinement of a vitrification protocol for protocorm-like bodies of Dendrobium sonia-28.
title_fullStr Refinement of a vitrification protocol for protocorm-like bodies of Dendrobium sonia-28.
title_full_unstemmed Refinement of a vitrification protocol for protocorm-like bodies of Dendrobium sonia-28.
title_short Refinement of a vitrification protocol for protocorm-like bodies of Dendrobium sonia-28.
title_sort refinement of a vitrification protocol for protocorm like bodies of dendrobium sonia 28
url http://psasir.upm.edu.my/id/eprint/28115/1/Refinement%20of%20a%20vitrification%20protocol%20for%20protocorm.pdf
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