Refinement of a vitrification protocol for protocorm-like bodies of Dendrobium sonia-28.
The hybrid Dendrobium sonia-28 is an important ornamental orchid in the Malaysian flower industry. However, it faces the risk of producing heterozygous progenies through conventional seed propagation or somaclonal variation. Cryopreservation is a favoured long-term storage method in the preserva...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Scientific and Technical Research Council of Turkey
2013
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/28115/1/Refinement%20of%20a%20vitrification%20protocol%20for%20protocorm.pdf |
| _version_ | 1825947253152415744 |
|---|---|
| author | Poobathy, Ranjetta Sinniah, Uma Rani Mahmood, Maziah Subramaniam, Sreeramanan |
| author_facet | Poobathy, Ranjetta Sinniah, Uma Rani Mahmood, Maziah Subramaniam, Sreeramanan |
| author_sort | Poobathy, Ranjetta |
| collection | UPM |
| description | The hybrid Dendrobium sonia-28 is an important ornamental orchid in the Malaysian flower industry. However, it faces the risk
of producing heterozygous progenies through conventional seed propagation or somaclonal variation. Cryopreservation is a favoured
long-term storage method in the preservation and maintenance of orchids with specific desired traits. This paper presents a successful
cryopreservation protocol for protocorm-like bodies (PLBs) of Dendrobium sonia-28 through the vitrification technique and the use
of ascorbic acid and charcoal. Survival assessments were conducted through growth evaluations and visual and spectrophotometric
2,3,5-triphenyltetrazolium chloride assays. Results of this study, an improvement over the previous protocol applied to this orchid
hybrid, showed that the addition of ascorbic acid to the preculture, loading, dehydration, unloading, and regeneration media, coupled
with the addition of charcoal to the regeneration medium, helped boost recovery rates of cryopreserved PLBs of Dendrobium sonia-28. |
| first_indexed | 2024-03-06T08:10:24Z |
| format | Article |
| id | upm.eprints-28115 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:10:24Z |
| publishDate | 2013 |
| publisher | Scientific and Technical Research Council of Turkey |
| record_format | dspace |
| spelling | upm.eprints-281152015-10-28T05:59:08Z http://psasir.upm.edu.my/id/eprint/28115/ Refinement of a vitrification protocol for protocorm-like bodies of Dendrobium sonia-28. Poobathy, Ranjetta Sinniah, Uma Rani Mahmood, Maziah Subramaniam, Sreeramanan The hybrid Dendrobium sonia-28 is an important ornamental orchid in the Malaysian flower industry. However, it faces the risk of producing heterozygous progenies through conventional seed propagation or somaclonal variation. Cryopreservation is a favoured long-term storage method in the preservation and maintenance of orchids with specific desired traits. This paper presents a successful cryopreservation protocol for protocorm-like bodies (PLBs) of Dendrobium sonia-28 through the vitrification technique and the use of ascorbic acid and charcoal. Survival assessments were conducted through growth evaluations and visual and spectrophotometric 2,3,5-triphenyltetrazolium chloride assays. Results of this study, an improvement over the previous protocol applied to this orchid hybrid, showed that the addition of ascorbic acid to the preculture, loading, dehydration, unloading, and regeneration media, coupled with the addition of charcoal to the regeneration medium, helped boost recovery rates of cryopreserved PLBs of Dendrobium sonia-28. Scientific and Technical Research Council of Turkey 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28115/1/Refinement%20of%20a%20vitrification%20protocol%20for%20protocorm.pdf Poobathy, Ranjetta and Sinniah, Uma Rani and Mahmood, Maziah and Subramaniam, Sreeramanan (2013) Refinement of a vitrification protocol for protocorm-like bodies of Dendrobium sonia-28. Turkish Journal of Botany, 37 (5). pp. 940-949. ISSN 1300-008X; ESSN: 1303-6106 http://mistug.tubitak.gov.tr/bdyim/toc.php?dergi=bot&yilsayi=2013/5 10.3906/bot-1208-35 |
| spellingShingle | Poobathy, Ranjetta Sinniah, Uma Rani Mahmood, Maziah Subramaniam, Sreeramanan Refinement of a vitrification protocol for protocorm-like bodies of Dendrobium sonia-28. |
| title | Refinement of a vitrification protocol for protocorm-like bodies of Dendrobium sonia-28. |
| title_full | Refinement of a vitrification protocol for protocorm-like bodies of Dendrobium sonia-28. |
| title_fullStr | Refinement of a vitrification protocol for protocorm-like bodies of Dendrobium sonia-28. |
| title_full_unstemmed | Refinement of a vitrification protocol for protocorm-like bodies of Dendrobium sonia-28. |
| title_short | Refinement of a vitrification protocol for protocorm-like bodies of Dendrobium sonia-28. |
| title_sort | refinement of a vitrification protocol for protocorm like bodies of dendrobium sonia 28 |
| url | http://psasir.upm.edu.my/id/eprint/28115/1/Refinement%20of%20a%20vitrification%20protocol%20for%20protocorm.pdf |
| work_keys_str_mv | AT poobathyranjetta refinementofavitrificationprotocolforprotocormlikebodiesofdendrobiumsonia28 AT sinniahumarani refinementofavitrificationprotocolforprotocormlikebodiesofdendrobiumsonia28 AT mahmoodmaziah refinementofavitrificationprotocolforprotocormlikebodiesofdendrobiumsonia28 AT subramaniamsreeramanan refinementofavitrificationprotocolforprotocormlikebodiesofdendrobiumsonia28 |