Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples.
On-chip detection of low abundant protein biomarkers is of interest to enable point-of-care diagnostics. Using a simple form of integration, we have realized an integrated microfluidic platform for the detection of prostate specific antigen (PSA), directly in anti-coagulated whole blood. We combine...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Royal Society of Chemistry
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/28121/1/Integrated%20acoustic%20immunoaffinity.pdf |
| _version_ | 1825947254363521024 |
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| author | Ahmad Tajudin, Asilah Petersson, K. Lenshof, A. Sward-Nilsson, A. M. Aberg, L. Marko-Varga, G. Malm, J. Lilja, H. Laurell, T. |
| author_facet | Ahmad Tajudin, Asilah Petersson, K. Lenshof, A. Sward-Nilsson, A. M. Aberg, L. Marko-Varga, G. Malm, J. Lilja, H. Laurell, T. |
| author_sort | Ahmad Tajudin, Asilah |
| collection | UPM |
| description | On-chip detection of low abundant protein biomarkers is of interest to enable point-of-care diagnostics. Using a simple form of integration, we have realized an integrated microfluidic platform for the detection of prostate specific antigen (PSA), directly in anti-coagulated whole blood. We combine acoustophoresis-based separation of plasma from undiluted whole blood with a miniaturized immunoassay system in a polymer manifold, demonstrating improved assay speed on our Integrated Acoustic Immunoaffinity-capture (IAI) platform. The IAI platform separates plasma from undiluted whole blood by means of acoustophoresis and provides cell free plasma of clinical quality at a rate of 10 uL/min for an online immunoaffinity-capture of PSA on a porous silicon antibody microarray. The whole blood input (hematocrit 38-40%) rate was 50 μl min(-1) giving a plasma volume fraction yield of ≈33%. PSA was immunoaffinity-captured directly from spiked female whole blood samples at clinically significant levels of 1.7-100 ng ml(-1) within 15 min and was subsequently detected via fluorescence readout, showing a linear response over the entire range with a coefficient of variation of 13%. |
| first_indexed | 2024-03-06T08:10:25Z |
| format | Article |
| id | upm.eprints-28121 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T08:10:25Z |
| publishDate | 2013 |
| publisher | Royal Society of Chemistry |
| record_format | dspace |
| spelling | upm.eprints-281212015-09-14T07:55:14Z http://psasir.upm.edu.my/id/eprint/28121/ Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples. Ahmad Tajudin, Asilah Petersson, K. Lenshof, A. Sward-Nilsson, A. M. Aberg, L. Marko-Varga, G. Malm, J. Lilja, H. Laurell, T. On-chip detection of low abundant protein biomarkers is of interest to enable point-of-care diagnostics. Using a simple form of integration, we have realized an integrated microfluidic platform for the detection of prostate specific antigen (PSA), directly in anti-coagulated whole blood. We combine acoustophoresis-based separation of plasma from undiluted whole blood with a miniaturized immunoassay system in a polymer manifold, demonstrating improved assay speed on our Integrated Acoustic Immunoaffinity-capture (IAI) platform. The IAI platform separates plasma from undiluted whole blood by means of acoustophoresis and provides cell free plasma of clinical quality at a rate of 10 uL/min for an online immunoaffinity-capture of PSA on a porous silicon antibody microarray. The whole blood input (hematocrit 38-40%) rate was 50 μl min(-1) giving a plasma volume fraction yield of ≈33%. PSA was immunoaffinity-captured directly from spiked female whole blood samples at clinically significant levels of 1.7-100 ng ml(-1) within 15 min and was subsequently detected via fluorescence readout, showing a linear response over the entire range with a coefficient of variation of 13%. Royal Society of Chemistry 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28121/1/Integrated%20acoustic%20immunoaffinity.pdf Ahmad Tajudin, Asilah and Petersson, K. and Lenshof, A. and Sward-Nilsson, A. M. and Aberg, L. and Marko-Varga, G. and Malm, J. and Lilja, H. and Laurell, T. (2013) Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples. Lab on a Chip, 13 (9). pp. 1790-1796. ISSN 1473-0197; ESSN: 1473-0189 10.1039/c3lc41269e English |
| spellingShingle | Ahmad Tajudin, Asilah Petersson, K. Lenshof, A. Sward-Nilsson, A. M. Aberg, L. Marko-Varga, G. Malm, J. Lilja, H. Laurell, T. Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples. |
| title | Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples. |
| title_full | Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples. |
| title_fullStr | Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples. |
| title_full_unstemmed | Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples. |
| title_short | Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples. |
| title_sort | integrated acoustic immunoaffinity capture iai platform for detection of psa from whole blood samples |
| url | http://psasir.upm.edu.my/id/eprint/28121/1/Integrated%20acoustic%20immunoaffinity.pdf |
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