Beneficial effects of parenteral GLP-1 delivery by cell therapy in insulin-deficient streptozotocin diabetic mice.
Parenteral delivery of long-Acting glucagon-like peptide-1 (GLP-1) mimetics has received much attention as a therapeutic option for diabetes. However, cell therapy-based GLP-1 treatments may provide a more physiological regulation of blood glucose. The present study assessed the effects of chronic G...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English |
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Nature Publishing Group
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/28127/1/Beneficial%20effects%20of%20parenteral%20GLP.pdf |
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| author | Vasu, Srividya Moffett, R. C. McCluskey, J. T. Hamid, Muhajir Irwin, N. Flatt, P. R. |
| author_facet | Vasu, Srividya Moffett, R. C. McCluskey, J. T. Hamid, Muhajir Irwin, N. Flatt, P. R. |
| author_sort | Vasu, Srividya |
| collection | UPM |
| description | Parenteral delivery of long-Acting glucagon-like peptide-1 (GLP-1) mimetics has received much attention as a therapeutic option for diabetes. However, cell therapy-based GLP-1 treatments may provide a more physiological regulation of blood glucose. The present study assessed the effects of chronic GLP-1 delivery by cell therapy, using the GLP-1-secreting GLUTag cell line, in normoglycemic and streptozotocin-induced diabetic mice. GLUTag cell aggregates were transplanted into the subscapular region of mice. Over 30 days, cellular transplantation gave rise to encapsulated and well-vascularized growths, which contained immunoreactive GLP-1. Cell implantation was well tolerated and had no appreciable metabolic effects in normal mice. However, transplantation significantly (P<0.001) countered excessive food and fluid intake in diabetic mice and maintained normal body weight. Circulating glucose (P<0.01) and glucagon (P<0.05) were significantly reduced and plasma insulin and GLP-1 dramatically increased. This was associated with significantly (P<0.01) improved glucose tolerance in diabetic mice. Histological examination of the pancreata of these mice revealed elevations (P<0.001) in islet and β-cell area, with reduced (P<0.001) -cell area. Increased β-cell mass reflected the enhanced proliferation relative to apoptosis. These studies emphasize the potential of chronic GLP-1 delivery by cell therapy as a potential therapeutic option for diabetes. |
| first_indexed | 2024-03-06T08:10:26Z |
| format | Article |
| id | upm.eprints-28127 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T08:10:26Z |
| publishDate | 2013 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | upm.eprints-281272015-10-07T03:54:11Z http://psasir.upm.edu.my/id/eprint/28127/ Beneficial effects of parenteral GLP-1 delivery by cell therapy in insulin-deficient streptozotocin diabetic mice. Vasu, Srividya Moffett, R. C. McCluskey, J. T. Hamid, Muhajir Irwin, N. Flatt, P. R. Parenteral delivery of long-Acting glucagon-like peptide-1 (GLP-1) mimetics has received much attention as a therapeutic option for diabetes. However, cell therapy-based GLP-1 treatments may provide a more physiological regulation of blood glucose. The present study assessed the effects of chronic GLP-1 delivery by cell therapy, using the GLP-1-secreting GLUTag cell line, in normoglycemic and streptozotocin-induced diabetic mice. GLUTag cell aggregates were transplanted into the subscapular region of mice. Over 30 days, cellular transplantation gave rise to encapsulated and well-vascularized growths, which contained immunoreactive GLP-1. Cell implantation was well tolerated and had no appreciable metabolic effects in normal mice. However, transplantation significantly (P<0.001) countered excessive food and fluid intake in diabetic mice and maintained normal body weight. Circulating glucose (P<0.01) and glucagon (P<0.05) were significantly reduced and plasma insulin and GLP-1 dramatically increased. This was associated with significantly (P<0.01) improved glucose tolerance in diabetic mice. Histological examination of the pancreata of these mice revealed elevations (P<0.001) in islet and β-cell area, with reduced (P<0.001) -cell area. Increased β-cell mass reflected the enhanced proliferation relative to apoptosis. These studies emphasize the potential of chronic GLP-1 delivery by cell therapy as a potential therapeutic option for diabetes. Nature Publishing Group 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28127/1/Beneficial%20effects%20of%20parenteral%20GLP.pdf Vasu, Srividya and Moffett, R. C. and McCluskey, J. T. and Hamid, Muhajir and Irwin, N. and Flatt, P. R. (2013) Beneficial effects of parenteral GLP-1 delivery by cell therapy in insulin-deficient streptozotocin diabetic mice. Gene Therapy, 20 (11). pp. 1077-1084. ISSN 0969-7128; ESSN: 1476-5462 http://www.nature.com/gt/journal/v20/n11/index.html 10.1038/gt.2013.33 English |
| spellingShingle | Vasu, Srividya Moffett, R. C. McCluskey, J. T. Hamid, Muhajir Irwin, N. Flatt, P. R. Beneficial effects of parenteral GLP-1 delivery by cell therapy in insulin-deficient streptozotocin diabetic mice. |
| title | Beneficial effects of parenteral GLP-1 delivery by cell therapy in insulin-deficient streptozotocin diabetic mice. |
| title_full | Beneficial effects of parenteral GLP-1 delivery by cell therapy in insulin-deficient streptozotocin diabetic mice. |
| title_fullStr | Beneficial effects of parenteral GLP-1 delivery by cell therapy in insulin-deficient streptozotocin diabetic mice. |
| title_full_unstemmed | Beneficial effects of parenteral GLP-1 delivery by cell therapy in insulin-deficient streptozotocin diabetic mice. |
| title_short | Beneficial effects of parenteral GLP-1 delivery by cell therapy in insulin-deficient streptozotocin diabetic mice. |
| title_sort | beneficial effects of parenteral glp 1 delivery by cell therapy in insulin deficient streptozotocin diabetic mice |
| url | http://psasir.upm.edu.my/id/eprint/28127/1/Beneficial%20effects%20of%20parenteral%20GLP.pdf |
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