Effects of ascorbic acid on PVS2 cryopreservation of dendrobium Bobby Messina’s PLBs supported with SEM analysis.
Regrowth of the cryopreserved protocorm-like bodies (PLBs) of Dendrobium Bobby Messina was assessed based on the plant vitrification solution 2 (PVS2) optimisation conditions. The optimized protocol obtained based on TTC spectrophotometrical analysis and growth recovery were 3–4 mm of PLBs size prec...
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| 格式: | 文件 |
| 语言: | English English |
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Humana Press
2013
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| 在线阅读: | http://psasir.upm.edu.my/id/eprint/28137/1/Effects%20of%20ascorbic%20acid%20on%20PVS2%20cryopreservation%20of%20dendrobium%20Bobby%20Messina.pdf |
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| author | James Antony, Jessica Jeyanthi Chan, Lai Keng Mahmood, Maziah Subramaniam, Sreeramanan |
| author_facet | James Antony, Jessica Jeyanthi Chan, Lai Keng Mahmood, Maziah Subramaniam, Sreeramanan |
| author_sort | James Antony, Jessica Jeyanthi |
| collection | UPM |
| description | Regrowth of the cryopreserved protocorm-like bodies (PLBs) of Dendrobium Bobby Messina was assessed based on the plant vitrification solution 2 (PVS2) optimisation conditions. The optimized protocol obtained based on TTC spectrophotometrical analysis and growth recovery were 3–4 mm of PLBs size precultured in 0.2 M sucrose for 1 day, treated with a mixture of 2 M glycerol and 0.4 M sucrose supplemented with half-strength liquid MS media at 25 °C for 20 min and subsequently dehydrated with PVS2 at 0 °C for 20 min prior to storage in liquid nitrogen. Following rapid warming in a water bath at 40 °C for 90 s, PLBs were treated with unloading solution containing half-strength liquid MS media supplemented with 1.2 M sucrose. Subsequently, the PLBs were cultured on half-strength semi-solid MS media supplemented with 2 % (w/v) sucrose without any growth regulators and resulted in 40 % growth recovery. In addition, ascorbic acid treatment was used to evaluate the regeneration process of cryopreserved PLBs. However, growth recovery rates of non-cryopreserved and cryopreserved PLBs were 30 and 10 % when 0.6 mM ascorbic acid was added. Scanning electron microscopy analysis indicates that there are not much damages observed on both cryopreserved and non-cryopreserved PLBs in comparison to PLBs stock culture. |
| first_indexed | 2024-03-06T08:10:28Z |
| format | Article |
| id | upm.eprints-28137 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T08:10:28Z |
| publishDate | 2013 |
| publisher | Humana Press |
| record_format | dspace |
| spelling | upm.eprints-281372015-10-05T06:19:42Z http://psasir.upm.edu.my/id/eprint/28137/ Effects of ascorbic acid on PVS2 cryopreservation of dendrobium Bobby Messina’s PLBs supported with SEM analysis. James Antony, Jessica Jeyanthi Chan, Lai Keng Mahmood, Maziah Subramaniam, Sreeramanan Regrowth of the cryopreserved protocorm-like bodies (PLBs) of Dendrobium Bobby Messina was assessed based on the plant vitrification solution 2 (PVS2) optimisation conditions. The optimized protocol obtained based on TTC spectrophotometrical analysis and growth recovery were 3–4 mm of PLBs size precultured in 0.2 M sucrose for 1 day, treated with a mixture of 2 M glycerol and 0.4 M sucrose supplemented with half-strength liquid MS media at 25 °C for 20 min and subsequently dehydrated with PVS2 at 0 °C for 20 min prior to storage in liquid nitrogen. Following rapid warming in a water bath at 40 °C for 90 s, PLBs were treated with unloading solution containing half-strength liquid MS media supplemented with 1.2 M sucrose. Subsequently, the PLBs were cultured on half-strength semi-solid MS media supplemented with 2 % (w/v) sucrose without any growth regulators and resulted in 40 % growth recovery. In addition, ascorbic acid treatment was used to evaluate the regeneration process of cryopreserved PLBs. However, growth recovery rates of non-cryopreserved and cryopreserved PLBs were 30 and 10 % when 0.6 mM ascorbic acid was added. Scanning electron microscopy analysis indicates that there are not much damages observed on both cryopreserved and non-cryopreserved PLBs in comparison to PLBs stock culture. Humana Press 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28137/1/Effects%20of%20ascorbic%20acid%20on%20PVS2%20cryopreservation%20of%20dendrobium%20Bobby%20Messina.pdf James Antony, Jessica Jeyanthi and Chan, Lai Keng and Mahmood, Maziah and Subramaniam, Sreeramanan (2013) Effects of ascorbic acid on PVS2 cryopreservation of dendrobium Bobby Messina’s PLBs supported with SEM analysis. Applied Biochemistry and Biotechnology, 171 (2). pp. 315-329. ISSN 0273-2289; ESSN: 1559-0291 English |
| spellingShingle | James Antony, Jessica Jeyanthi Chan, Lai Keng Mahmood, Maziah Subramaniam, Sreeramanan Effects of ascorbic acid on PVS2 cryopreservation of dendrobium Bobby Messina’s PLBs supported with SEM analysis. |
| title | Effects of ascorbic acid on PVS2 cryopreservation of dendrobium Bobby Messina’s PLBs supported with SEM analysis. |
| title_full | Effects of ascorbic acid on PVS2 cryopreservation of dendrobium Bobby Messina’s PLBs supported with SEM analysis. |
| title_fullStr | Effects of ascorbic acid on PVS2 cryopreservation of dendrobium Bobby Messina’s PLBs supported with SEM analysis. |
| title_full_unstemmed | Effects of ascorbic acid on PVS2 cryopreservation of dendrobium Bobby Messina’s PLBs supported with SEM analysis. |
| title_short | Effects of ascorbic acid on PVS2 cryopreservation of dendrobium Bobby Messina’s PLBs supported with SEM analysis. |
| title_sort | effects of ascorbic acid on pvs2 cryopreservation of dendrobium bobby messina s plbs supported with sem analysis |
| url | http://psasir.upm.edu.my/id/eprint/28137/1/Effects%20of%20ascorbic%20acid%20on%20PVS2%20cryopreservation%20of%20dendrobium%20Bobby%20Messina.pdf |
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