Phytochemical screening and antioxidant activity assessment of the leaf stem and root of (Labisia paucifolia)
Researches have focused on medicinal plants in order to extract natural and low cost antioxidants that can help to protect the human body against oxidative stress and replace the synthetic additives that might be carcinogenic. In the present study, the crude extracts of leaves, stems and roots of La...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Southern Cross Publishing
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29217/1/Phytochemical%20screening%20and%20antioxidant%20activity%20assessment%20of%20the%20leaf%20stem%20and%20root%20of%20%28Labisia%20paucifolia%29.pdf |
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| author | Jaafar, Hawa Z. E. Karimi, Ehsan Ibrahim, Mohd Hafiz Daghigh, Ali Ghasemzadeh |
| author_facet | Jaafar, Hawa Z. E. Karimi, Ehsan Ibrahim, Mohd Hafiz Daghigh, Ali Ghasemzadeh |
| author_sort | Jaafar, Hawa Z. E. |
| collection | UPM |
| description | Researches have focused on medicinal plants in order to extract natural and low cost antioxidants that can help to protect the human body against oxidative stress and replace the synthetic additives that might be carcinogenic. In the present study, the crude extracts of leaves, stems and roots of Labisia paucifolia were investigated for their phytochemical constituents and antioxidant activities. The chemical compositions of bioactive compounds were determined using HPLC and GC-MS and the antioxidant activities measured by DPPH and FRAP methods. The results indicated that leaves methanolic extract had highest total phenolic and flavonoid contents with respective values of 2.51 mg gallic acid equivalent g DW-1 and 1.29 mg rutin equivalent g DW-1, compared to roots and stems. Meanwhile, the HPLC analysis showed the presence of gallic acid and kaempferol as the major phenolic and flavonoid compounds in all parts of L. paucifolia. Other metabolites were detected by GC-MS screening including 9,12,15-Octadecatrienoic acid, methyl ester (22.72%), 4H-Pyran-4-0ne,2,3-dihydro-35-dihydroxy-6-methyl (7.93%) and acetic acid (9.02%) as the main compounds in the leaf, stem and root extracts of L. paucifolia. The extracts possess antioxidant activity because the free radical scavenging and ferric reducing power activities were higher in leaf extract at a concentration of 500 μg mL-1 with values of 53% and 51%, respectively, as compared to the stem and root, but the activities were around 32-53% lower than those of antioxidant standards such as BHT and α-tocopherol. The present result revealed that L. paucifolia extracts contain variable patterns of flavonoids, phenolics and various bioactive volatile compounds and it could be applied as a natural antioxidant source for industrial purposes. |
| first_indexed | 2024-03-06T08:13:51Z |
| format | Article |
| id | upm.eprints-29217 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:13:51Z |
| publishDate | 2013 |
| publisher | Southern Cross Publishing |
| record_format | dspace |
| spelling | upm.eprints-292172016-06-20T07:05:22Z http://psasir.upm.edu.my/id/eprint/29217/ Phytochemical screening and antioxidant activity assessment of the leaf stem and root of (Labisia paucifolia) Jaafar, Hawa Z. E. Karimi, Ehsan Ibrahim, Mohd Hafiz Daghigh, Ali Ghasemzadeh Researches have focused on medicinal plants in order to extract natural and low cost antioxidants that can help to protect the human body against oxidative stress and replace the synthetic additives that might be carcinogenic. In the present study, the crude extracts of leaves, stems and roots of Labisia paucifolia were investigated for their phytochemical constituents and antioxidant activities. The chemical compositions of bioactive compounds were determined using HPLC and GC-MS and the antioxidant activities measured by DPPH and FRAP methods. The results indicated that leaves methanolic extract had highest total phenolic and flavonoid contents with respective values of 2.51 mg gallic acid equivalent g DW-1 and 1.29 mg rutin equivalent g DW-1, compared to roots and stems. Meanwhile, the HPLC analysis showed the presence of gallic acid and kaempferol as the major phenolic and flavonoid compounds in all parts of L. paucifolia. Other metabolites were detected by GC-MS screening including 9,12,15-Octadecatrienoic acid, methyl ester (22.72%), 4H-Pyran-4-0ne,2,3-dihydro-35-dihydroxy-6-methyl (7.93%) and acetic acid (9.02%) as the main compounds in the leaf, stem and root extracts of L. paucifolia. The extracts possess antioxidant activity because the free radical scavenging and ferric reducing power activities were higher in leaf extract at a concentration of 500 μg mL-1 with values of 53% and 51%, respectively, as compared to the stem and root, but the activities were around 32-53% lower than those of antioxidant standards such as BHT and α-tocopherol. The present result revealed that L. paucifolia extracts contain variable patterns of flavonoids, phenolics and various bioactive volatile compounds and it could be applied as a natural antioxidant source for industrial purposes. Southern Cross Publishing 2013-02 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29217/1/Phytochemical%20screening%20and%20antioxidant%20activity%20assessment%20of%20the%20leaf%20stem%20and%20root%20of%20%28Labisia%20paucifolia%29.pdf Jaafar, Hawa Z. E. and Karimi, Ehsan and Ibrahim, Mohd Hafiz and Daghigh, Ali Ghasemzadeh (2013) Phytochemical screening and antioxidant activity assessment of the leaf stem and root of (Labisia paucifolia). Australian Journal of Crop Science, 7 (2). pp. 276-280. ISSN 1835-2693; ESSN: 1835-2707 http://www.cropj.com/february2013.html |
| spellingShingle | Jaafar, Hawa Z. E. Karimi, Ehsan Ibrahim, Mohd Hafiz Daghigh, Ali Ghasemzadeh Phytochemical screening and antioxidant activity assessment of the leaf stem and root of (Labisia paucifolia) |
| title | Phytochemical screening and antioxidant activity assessment of the leaf stem and root of (Labisia paucifolia) |
| title_full | Phytochemical screening and antioxidant activity assessment of the leaf stem and root of (Labisia paucifolia) |
| title_fullStr | Phytochemical screening and antioxidant activity assessment of the leaf stem and root of (Labisia paucifolia) |
| title_full_unstemmed | Phytochemical screening and antioxidant activity assessment of the leaf stem and root of (Labisia paucifolia) |
| title_short | Phytochemical screening and antioxidant activity assessment of the leaf stem and root of (Labisia paucifolia) |
| title_sort | phytochemical screening and antioxidant activity assessment of the leaf stem and root of labisia paucifolia |
| url | http://psasir.upm.edu.my/id/eprint/29217/1/Phytochemical%20screening%20and%20antioxidant%20activity%20assessment%20of%20the%20leaf%20stem%20and%20root%20of%20%28Labisia%20paucifolia%29.pdf |
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