Inter- and intra-population genetic variations in Jatropha curcas populations revealed by inter-simple sequence repeat molecular markers
Inter simple sequence reaction (ISSR) was employed to assess diversity in six Jatropha populations that were collected in Malaysia, Indonesia, the Philippines and India. A total of 144 accessions (24 accessions per population) were selected and analysed with 10 ISSR primer combinations to generate a...
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| Format: | Article |
| Language: | English |
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Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29255/1/58_111.pdf |
| _version_ | 1825947476778024960 |
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| author | Biabani, Alireza Yusop, Mohd Rafii Saleh, Ghizan Latif, Mohammad Abdul |
| author_facet | Biabani, Alireza Yusop, Mohd Rafii Saleh, Ghizan Latif, Mohammad Abdul |
| author_sort | Biabani, Alireza |
| collection | UPM |
| description | Inter simple sequence reaction (ISSR) was employed to assess diversity in six Jatropha populations that were collected in Malaysia, Indonesia, the Philippines and India. A total of 144 accessions (24 accessions per population) were selected and analysed with 10 ISSR primer combinations to generate a total of 143 polymorphic fragments. The number of bands generated per primer varied from 4 to 27. The percentages of polymorphic bands for the Indonesia1, Indonesia2, Malaysia1, Malaysia2, Philippines and India Jatropha populations were 54.6, 59.4, 46.2, 53.2, 60.8 and 56.4%, respectively, with an average of 55.1%. The Nei's total genetic diversity (HT), the intra-populations genetic diversity (HS) and the coefficient of genetic differentiation (GST) were 0.1136, 0.0989 and 0.1295, respectively. The coefficient of genetic differentiation (GST) was 0.1295, which indicated a frequency of approximately 13% in genetic variations observed in the inter-Jatropha populations, while an 87% variation corresponded to the intra-Jatropha populations. Analysis of molecular variance (AMOVA) analysis indicated highly significant (p ≤ 0.001) genetic differences among the six Jatropha populations. An UPGMA dendrogram was constructed, and the Jatropha populations were grouped into four major clusters at a coefficient level of 0.28. The genetic similarities between the populations ranged from 0.31 to 0.25. The principal component analysis showed a relatively similar grouping of the populations. To obtain high heterotic responses, hybridisation should be made between the two distant populations of Malaysia 1 and Indonesia1. |
| first_indexed | 2024-03-06T08:13:58Z |
| format | Article |
| id | upm.eprints-29255 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:13:58Z |
| publishDate | 2013 |
| publisher | Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria |
| record_format | dspace |
| spelling | upm.eprints-292552017-11-10T05:17:51Z http://psasir.upm.edu.my/id/eprint/29255/ Inter- and intra-population genetic variations in Jatropha curcas populations revealed by inter-simple sequence repeat molecular markers Biabani, Alireza Yusop, Mohd Rafii Saleh, Ghizan Latif, Mohammad Abdul Inter simple sequence reaction (ISSR) was employed to assess diversity in six Jatropha populations that were collected in Malaysia, Indonesia, the Philippines and India. A total of 144 accessions (24 accessions per population) were selected and analysed with 10 ISSR primer combinations to generate a total of 143 polymorphic fragments. The number of bands generated per primer varied from 4 to 27. The percentages of polymorphic bands for the Indonesia1, Indonesia2, Malaysia1, Malaysia2, Philippines and India Jatropha populations were 54.6, 59.4, 46.2, 53.2, 60.8 and 56.4%, respectively, with an average of 55.1%. The Nei's total genetic diversity (HT), the intra-populations genetic diversity (HS) and the coefficient of genetic differentiation (GST) were 0.1136, 0.0989 and 0.1295, respectively. The coefficient of genetic differentiation (GST) was 0.1295, which indicated a frequency of approximately 13% in genetic variations observed in the inter-Jatropha populations, while an 87% variation corresponded to the intra-Jatropha populations. Analysis of molecular variance (AMOVA) analysis indicated highly significant (p ≤ 0.001) genetic differences among the six Jatropha populations. An UPGMA dendrogram was constructed, and the Jatropha populations were grouped into four major clusters at a coefficient level of 0.28. The genetic similarities between the populations ranged from 0.31 to 0.25. The principal component analysis showed a relatively similar grouping of the populations. To obtain high heterotic responses, hybridisation should be made between the two distant populations of Malaysia 1 and Indonesia1. Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29255/1/58_111.pdf Biabani, Alireza and Yusop, Mohd Rafii and Saleh, Ghizan and Latif, Mohammad Abdul (2013) Inter- and intra-population genetic variations in Jatropha curcas populations revealed by inter-simple sequence repeat molecular markers. Maydica, 58 (2). pp. 111-118. ISSN 2279-8013 http://www.maydica.org/maydica2013.html |
| spellingShingle | Biabani, Alireza Yusop, Mohd Rafii Saleh, Ghizan Latif, Mohammad Abdul Inter- and intra-population genetic variations in Jatropha curcas populations revealed by inter-simple sequence repeat molecular markers |
| title | Inter- and intra-population genetic variations in Jatropha curcas populations revealed by inter-simple sequence repeat molecular markers |
| title_full | Inter- and intra-population genetic variations in Jatropha curcas populations revealed by inter-simple sequence repeat molecular markers |
| title_fullStr | Inter- and intra-population genetic variations in Jatropha curcas populations revealed by inter-simple sequence repeat molecular markers |
| title_full_unstemmed | Inter- and intra-population genetic variations in Jatropha curcas populations revealed by inter-simple sequence repeat molecular markers |
| title_short | Inter- and intra-population genetic variations in Jatropha curcas populations revealed by inter-simple sequence repeat molecular markers |
| title_sort | inter and intra population genetic variations in jatropha curcas populations revealed by inter simple sequence repeat molecular markers |
| url | http://psasir.upm.edu.my/id/eprint/29255/1/58_111.pdf |
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