Effect of PVS2 vitrification on Brassidium shooting star orchid using protocorm-like bodies (PLBs)
A cryopreservation procedure was developed to preserve protocorm-like bodies (PLBs) of Brassidium Shooting Star using the PVS2 vitrification technique. The optimised protocol involved the preculture of 3-4mm PLBs in half-strength Murashige and Skoog (MS) semi-solid medium supplemented with 0.8M sucr...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Southern Cross Publishing
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29390/1/Effect%20of%20PVS2%20vitrification%20on%20Brassidium%20shooting%20star%20orchid%20using%20protocorm-like%20bodies%20%28PLBs%29.pdf |
| _version_ | 1825947502998716416 |
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| author | James Antony, Jessica Jeyanthi Burkhan, Hazirah Sinniah, Uma Rani Poobathy, Ranjetta Subramaniam, Sreeramanan |
| author_facet | James Antony, Jessica Jeyanthi Burkhan, Hazirah Sinniah, Uma Rani Poobathy, Ranjetta Subramaniam, Sreeramanan |
| author_sort | James Antony, Jessica Jeyanthi |
| collection | UPM |
| description | A cryopreservation procedure was developed to preserve protocorm-like bodies (PLBs) of Brassidium Shooting Star using the PVS2 vitrification technique. The optimised protocol involved the preculture of 3-4mm PLBs in half-strength Murashige and Skoog (MS) semi-solid medium supplemented with 0.8M sucrose, followed by dehydration in PVS2 solution for 20 minutes at 0°C, prior to storage in liquid nitrogen. The viability of non-cryopreserved and cryopreserved PLBs was determined by the 2,3,5-triphenyltetrazolium chloride (TTC) assay, after two weeks of recovery. The chlorophyll contents, total soluble protein and peroxidase activities of both non-cryopreserved and cryopreserved PLBs were assayed after three weeks of recovery. The results from the biochemical analyses indicated that control PLBs produced the highest viability, followed by treatment on non-cryopreserved PLBs (-LN) and cryostored PLBs (+LN), except in the peroxidase activity assay. The peroxidase activity was detected as the highest in cryostored PLBs followed by treated but non-cryopreserved PLBs, and control PLBs. |
| first_indexed | 2024-03-06T08:14:22Z |
| format | Article |
| id | upm.eprints-29390 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:14:22Z |
| publishDate | 2013 |
| publisher | Southern Cross Publishing |
| record_format | dspace |
| spelling | upm.eprints-293902016-07-13T01:44:21Z http://psasir.upm.edu.my/id/eprint/29390/ Effect of PVS2 vitrification on Brassidium shooting star orchid using protocorm-like bodies (PLBs) James Antony, Jessica Jeyanthi Burkhan, Hazirah Sinniah, Uma Rani Poobathy, Ranjetta Subramaniam, Sreeramanan A cryopreservation procedure was developed to preserve protocorm-like bodies (PLBs) of Brassidium Shooting Star using the PVS2 vitrification technique. The optimised protocol involved the preculture of 3-4mm PLBs in half-strength Murashige and Skoog (MS) semi-solid medium supplemented with 0.8M sucrose, followed by dehydration in PVS2 solution for 20 minutes at 0°C, prior to storage in liquid nitrogen. The viability of non-cryopreserved and cryopreserved PLBs was determined by the 2,3,5-triphenyltetrazolium chloride (TTC) assay, after two weeks of recovery. The chlorophyll contents, total soluble protein and peroxidase activities of both non-cryopreserved and cryopreserved PLBs were assayed after three weeks of recovery. The results from the biochemical analyses indicated that control PLBs produced the highest viability, followed by treatment on non-cryopreserved PLBs (-LN) and cryostored PLBs (+LN), except in the peroxidase activity assay. The peroxidase activity was detected as the highest in cryostored PLBs followed by treated but non-cryopreserved PLBs, and control PLBs. Southern Cross Publishing 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29390/1/Effect%20of%20PVS2%20vitrification%20on%20Brassidium%20shooting%20star%20orchid%20using%20protocorm-like%20bodies%20%28PLBs%29.pdf James Antony, Jessica Jeyanthi and Burkhan, Hazirah and Sinniah, Uma Rani and Poobathy, Ranjetta and Subramaniam, Sreeramanan (2013) Effect of PVS2 vitrification on Brassidium shooting star orchid using protocorm-like bodies (PLBs). Australian Journal of Crop Science, 7 (8). pp. 1078-1084. ISSN 1835-2693; ESSN: 1835-2707 http://www.cropj.com/july2013.html |
| spellingShingle | James Antony, Jessica Jeyanthi Burkhan, Hazirah Sinniah, Uma Rani Poobathy, Ranjetta Subramaniam, Sreeramanan Effect of PVS2 vitrification on Brassidium shooting star orchid using protocorm-like bodies (PLBs) |
| title | Effect of PVS2 vitrification on Brassidium shooting star orchid using protocorm-like bodies (PLBs) |
| title_full | Effect of PVS2 vitrification on Brassidium shooting star orchid using protocorm-like bodies (PLBs) |
| title_fullStr | Effect of PVS2 vitrification on Brassidium shooting star orchid using protocorm-like bodies (PLBs) |
| title_full_unstemmed | Effect of PVS2 vitrification on Brassidium shooting star orchid using protocorm-like bodies (PLBs) |
| title_short | Effect of PVS2 vitrification on Brassidium shooting star orchid using protocorm-like bodies (PLBs) |
| title_sort | effect of pvs2 vitrification on brassidium shooting star orchid using protocorm like bodies plbs |
| url | http://psasir.upm.edu.my/id/eprint/29390/1/Effect%20of%20PVS2%20vitrification%20on%20Brassidium%20shooting%20star%20orchid%20using%20protocorm-like%20bodies%20%28PLBs%29.pdf |
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