Preliminary assessment of differential expression of candidate genes associated with atherosclerosis
Identifying susceptible genes associated with the pathogenesis of atherosclerosis (ATH) may contribute toward better management of this condition. This preliminary study was aimed at assessing the expression levels of 11 candidate genes, namely tumor protein (TP53), transforming growth factor, beta...
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| Format: | Article |
| Language: | English |
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The Genetics Society of Japan
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29461/1/Preliminary%20assessment%20of%20differential%20expression%20of%20candidate%20genes%20associated%20with%20atherosclerosis.pdf |
| _version_ | 1796971544018157568 |
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| author | Blin, Joan Ahmad, Zalinah Gyanchand Rampal, Lekhraj Rampal Mohtarrudin, Norhafizah Tajudin, Abdul Karim Shahar Adnan, Rohayu |
| author_facet | Blin, Joan Ahmad, Zalinah Gyanchand Rampal, Lekhraj Rampal Mohtarrudin, Norhafizah Tajudin, Abdul Karim Shahar Adnan, Rohayu |
| author_sort | Blin, Joan |
| collection | UPM |
| description | Identifying susceptible genes associated with the pathogenesis of atherosclerosis (ATH) may contribute toward better management of this condition. This preliminary study was aimed at assessing the expression levels of 11 candidate genes, namely tumor protein (TP53), transforming growth factor, beta receptor II (TGFBR2), cysthathionenine-beta-synthase (CBS), insulin receptor substrate 1 (IRS1), lipoprotein lipase (LPL), methylenetetrahydrofolate reductase (MTHFR), thrombomodulin (THBD), lecithin-cholesterol acyltransferase (LCAT), matrix metallopeptidase 9 (MMP9), low density lipoprotein receptor (LDLR), and arachidonate 5-lipoxygenase-activating protein (ALOX5AP) genes associated with ATH. Twelve human coronary artery tissues (HCATs) were obtained from deceased subjects who underwent post-mortem procedures. Six atherosclerotic coronary artery tissue (ACAT) samples representing the cases and non-atherosclerotic coronary artery tissue (NCAT) samples as controls were gathered based on predetermined inclusion and exclusion criteria. Gene expression levels were assessed using the GenomeLab Genetic Analysis System (GeXP). The results showed that LDLR, TP53, and MMP9 expression levels were significantly increased in ACAT compared to NCAT samples (p < 0.05). Thus, LDLR, TP53, and MMP9 genes may play important roles in the development of ATH in a Malaysian study population. |
| first_indexed | 2024-03-06T08:14:34Z |
| format | Article |
| id | upm.eprints-29461 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:14:34Z |
| publishDate | 2013 |
| publisher | The Genetics Society of Japan |
| record_format | dspace |
| spelling | upm.eprints-294612016-07-12T09:36:44Z http://psasir.upm.edu.my/id/eprint/29461/ Preliminary assessment of differential expression of candidate genes associated with atherosclerosis Blin, Joan Ahmad, Zalinah Gyanchand Rampal, Lekhraj Rampal Mohtarrudin, Norhafizah Tajudin, Abdul Karim Shahar Adnan, Rohayu Identifying susceptible genes associated with the pathogenesis of atherosclerosis (ATH) may contribute toward better management of this condition. This preliminary study was aimed at assessing the expression levels of 11 candidate genes, namely tumor protein (TP53), transforming growth factor, beta receptor II (TGFBR2), cysthathionenine-beta-synthase (CBS), insulin receptor substrate 1 (IRS1), lipoprotein lipase (LPL), methylenetetrahydrofolate reductase (MTHFR), thrombomodulin (THBD), lecithin-cholesterol acyltransferase (LCAT), matrix metallopeptidase 9 (MMP9), low density lipoprotein receptor (LDLR), and arachidonate 5-lipoxygenase-activating protein (ALOX5AP) genes associated with ATH. Twelve human coronary artery tissues (HCATs) were obtained from deceased subjects who underwent post-mortem procedures. Six atherosclerotic coronary artery tissue (ACAT) samples representing the cases and non-atherosclerotic coronary artery tissue (NCAT) samples as controls were gathered based on predetermined inclusion and exclusion criteria. Gene expression levels were assessed using the GenomeLab Genetic Analysis System (GeXP). The results showed that LDLR, TP53, and MMP9 expression levels were significantly increased in ACAT compared to NCAT samples (p < 0.05). Thus, LDLR, TP53, and MMP9 genes may play important roles in the development of ATH in a Malaysian study population. The Genetics Society of Japan 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29461/1/Preliminary%20assessment%20of%20differential%20expression%20of%20candidate%20genes%20associated%20with%20atherosclerosis.pdf Blin, Joan and Ahmad, Zalinah and Gyanchand Rampal, Lekhraj Rampal and Mohtarrudin, Norhafizah and Tajudin, Abdul Karim and Shahar Adnan, Rohayu (2013) Preliminary assessment of differential expression of candidate genes associated with atherosclerosis. Genes & Genetic Systems, 88 (3). pp. 199-209. ISSN 1341-7568; ESSN: 1880-5779 https://www.jstage.jst.go.jp/article/ggs/88/3/88_199/_article 10.1266/ggs.88.199 |
| spellingShingle | Blin, Joan Ahmad, Zalinah Gyanchand Rampal, Lekhraj Rampal Mohtarrudin, Norhafizah Tajudin, Abdul Karim Shahar Adnan, Rohayu Preliminary assessment of differential expression of candidate genes associated with atherosclerosis |
| title | Preliminary assessment of differential expression of candidate genes associated with atherosclerosis |
| title_full | Preliminary assessment of differential expression of candidate genes associated with atherosclerosis |
| title_fullStr | Preliminary assessment of differential expression of candidate genes associated with atherosclerosis |
| title_full_unstemmed | Preliminary assessment of differential expression of candidate genes associated with atherosclerosis |
| title_short | Preliminary assessment of differential expression of candidate genes associated with atherosclerosis |
| title_sort | preliminary assessment of differential expression of candidate genes associated with atherosclerosis |
| url | http://psasir.upm.edu.my/id/eprint/29461/1/Preliminary%20assessment%20of%20differential%20expression%20of%20candidate%20genes%20associated%20with%20atherosclerosis.pdf |
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