Isolation of a quinone-rich fraction from Ardisia crispa roots and its attenuating effects on murine skin tumorigenesis
Ardisia crispa (Family: Myrsinaceae) is an evergreen, fruiting shrub that has been traditionally used as folklore medicine. Despite a scarcity of research publications, we have succeeded in showing suppressive effects on murine skin papillomagenesis. In extension, the present research was aimed at d...
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| Format: | Article |
| Language: | English |
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Asian Pacific Organization for Cancer Prevention
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29463/1/Isolation%20of%20a%20quinone-rich%20fraction%20from%20Ardisia%20crispa%20roots%20and%20its%20attenuating%20effects%20on%20murine%20skin%20tumorigenesis.pdf |
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| author | Yeong, Looi Ting Abd Hamid @ Abdul Razak, Roslida Saiful Yazan, Latifah Khaza'ai, Huzwah |
| author_facet | Yeong, Looi Ting Abd Hamid @ Abdul Razak, Roslida Saiful Yazan, Latifah Khaza'ai, Huzwah |
| author_sort | Yeong, Looi Ting |
| collection | UPM |
| description | Ardisia crispa (Family: Myrsinaceae) is an evergreen, fruiting shrub that has been traditionally used as folklore medicine. Despite a scarcity of research publications, we have succeeded in showing suppressive effects on murine skin papillomagenesis. In extension, the present research was aimed at determining the effect of a quinone-rich fraction (QRF) isolated from the same root hexane extract on both initiation and promotion stages of carcinogenesis, at the selected dose of 30 mg/kg. Mice (groups I-IV) were initiated with a single dose of 7,12-dimethylbenz(a)anthracene (DMBA, 100 μg/100 μl) followed by repeated promotion of croton oil (1%) twice weekly for 20 weeks. In addition, group I (anti-initiation) received QRF 7 days before and after DMBA; group II (anti-promotion) received QRF 30 minutes before each croton oil application; group III (anti-initiation/ promotion) was treated with QRF as a combination of group I and II. A further two groups served as vehicle control (group V) and treated control (group VI). As carcinogen control, group IV showed the highest tumor volume (8.79±5.44) and tumor burden (3.60±1.17). Comparatively, group III revealed only 20% of tumor incidence, tumor burden (3.00±1.00) and tumor volume (2.40±1.12), which were significantly different from group IV. Group II also showed significant reduction of tumor volume (3.11), tumor burden (3.00) and tumor incidence (11.11%), along with prominent increase of latency period of tumor formation (week 12). Group I, nonetheless, demonstrated marked increment of tumor incidence by 40% with prompted latency period of tumor formation (week 7). No tumor formation was observed in groups V and VI. This study provided clear evidence of inhibitory effects of QRF during promotion period which was in agreement with our previous findings. The mechanism(s) underlying such effects have yet to be elucidated. |
| first_indexed | 2024-03-06T08:14:35Z |
| format | Article |
| id | upm.eprints-29463 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:14:35Z |
| publishDate | 2013 |
| publisher | Asian Pacific Organization for Cancer Prevention |
| record_format | dspace |
| spelling | upm.eprints-294632016-10-28T03:05:35Z http://psasir.upm.edu.my/id/eprint/29463/ Isolation of a quinone-rich fraction from Ardisia crispa roots and its attenuating effects on murine skin tumorigenesis Yeong, Looi Ting Abd Hamid @ Abdul Razak, Roslida Saiful Yazan, Latifah Khaza'ai, Huzwah Ardisia crispa (Family: Myrsinaceae) is an evergreen, fruiting shrub that has been traditionally used as folklore medicine. Despite a scarcity of research publications, we have succeeded in showing suppressive effects on murine skin papillomagenesis. In extension, the present research was aimed at determining the effect of a quinone-rich fraction (QRF) isolated from the same root hexane extract on both initiation and promotion stages of carcinogenesis, at the selected dose of 30 mg/kg. Mice (groups I-IV) were initiated with a single dose of 7,12-dimethylbenz(a)anthracene (DMBA, 100 μg/100 μl) followed by repeated promotion of croton oil (1%) twice weekly for 20 weeks. In addition, group I (anti-initiation) received QRF 7 days before and after DMBA; group II (anti-promotion) received QRF 30 minutes before each croton oil application; group III (anti-initiation/ promotion) was treated with QRF as a combination of group I and II. A further two groups served as vehicle control (group V) and treated control (group VI). As carcinogen control, group IV showed the highest tumor volume (8.79±5.44) and tumor burden (3.60±1.17). Comparatively, group III revealed only 20% of tumor incidence, tumor burden (3.00±1.00) and tumor volume (2.40±1.12), which were significantly different from group IV. Group II also showed significant reduction of tumor volume (3.11), tumor burden (3.00) and tumor incidence (11.11%), along with prominent increase of latency period of tumor formation (week 12). Group I, nonetheless, demonstrated marked increment of tumor incidence by 40% with prompted latency period of tumor formation (week 7). No tumor formation was observed in groups V and VI. This study provided clear evidence of inhibitory effects of QRF during promotion period which was in agreement with our previous findings. The mechanism(s) underlying such effects have yet to be elucidated. Asian Pacific Organization for Cancer Prevention 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29463/1/Isolation%20of%20a%20quinone-rich%20fraction%20from%20Ardisia%20crispa%20roots%20and%20its%20attenuating%20effects%20on%20murine%20skin%20tumorigenesis.pdf Yeong, Looi Ting and Abd Hamid @ Abdul Razak, Roslida and Saiful Yazan, Latifah and Khaza'ai, Huzwah (2013) Isolation of a quinone-rich fraction from Ardisia crispa roots and its attenuating effects on murine skin tumorigenesis. Asian Pacific Journal of Cancer Prevention, 14 (4). pp. 2301-2305. ISSN 1513-7368 http://koreascience.or.kr/article/ArticleFullRecord.jsp?cn=POCPA9_2013_v14n4_2301 10.7314/APJCP.2013.14.4.2301 |
| spellingShingle | Yeong, Looi Ting Abd Hamid @ Abdul Razak, Roslida Saiful Yazan, Latifah Khaza'ai, Huzwah Isolation of a quinone-rich fraction from Ardisia crispa roots and its attenuating effects on murine skin tumorigenesis |
| title | Isolation of a quinone-rich fraction from Ardisia crispa roots and its attenuating effects on murine skin tumorigenesis |
| title_full | Isolation of a quinone-rich fraction from Ardisia crispa roots and its attenuating effects on murine skin tumorigenesis |
| title_fullStr | Isolation of a quinone-rich fraction from Ardisia crispa roots and its attenuating effects on murine skin tumorigenesis |
| title_full_unstemmed | Isolation of a quinone-rich fraction from Ardisia crispa roots and its attenuating effects on murine skin tumorigenesis |
| title_short | Isolation of a quinone-rich fraction from Ardisia crispa roots and its attenuating effects on murine skin tumorigenesis |
| title_sort | isolation of a quinone rich fraction from ardisia crispa roots and its attenuating effects on murine skin tumorigenesis |
| url | http://psasir.upm.edu.my/id/eprint/29463/1/Isolation%20of%20a%20quinone-rich%20fraction%20from%20Ardisia%20crispa%20roots%20and%20its%20attenuating%20effects%20on%20murine%20skin%20tumorigenesis.pdf |
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