Seroprevalence and detection of contagious bovine pleuropneumonia (CBPP) in northeast states of Peninsular Malaysia
Contagious Bovine Pleuropneumonia (CBPP) is a highly infectious disease of cattle caused by Mycoplasma mycoides subsp. mycoides Small Colony (MmmSC). It is a disease recognised by OIE that needs to be controlled or eradicated through surveillance system. This study establishes the sero-prevalence of...
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| Format: | Article |
| Language: | English |
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Universiti Putra Malaysia Press
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29520/1/10%20JTAS-0773-2015.pdf |
| _version_ | 1796971555602825216 |
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| author | M., Zarina Saad, Mohd Zamri Hassan, Latiffah Salisi, Mohd Shahrom O., Norlida |
| author_facet | M., Zarina Saad, Mohd Zamri Hassan, Latiffah Salisi, Mohd Shahrom O., Norlida |
| author_sort | M., Zarina |
| collection | UPM |
| description | Contagious Bovine Pleuropneumonia (CBPP) is a highly infectious disease of cattle caused by Mycoplasma mycoides subsp. mycoides Small Colony (MmmSC). It is a disease recognised by OIE that needs to be controlled or eradicated through surveillance system. This study establishes the sero-prevalence of CBPP, as well as attempts to isolate and identify the agent of CBPP from cattle in Kelantan and Terengganu, the northeastern states of Peninsular Malaysia, where cattle movement was high. A total of 3,242 sera from 428 farms were processed between 2011 and 2014 using the competitive ELISA (c-ELISA). The animal-level prevalence ranged between 5% (46/917) in Terengganu and 9% (220/2325) in Kelantan, whereas the herd prevalence ranged between 12% (22/210) in Terengganu and 24% (53/218) in Kelantan. The overall animal-level prevalence was 8% (266/3242), while the herd prevalence was 17% (75/428). Two hundred and four nasal swabs from 18 positive herds in Kelantan and 163 lung and mediastinal lymph node samples were processed for isolation of MmmSc before confirmation by PCR and immunoperoxidase. Forty-one samples showed turbidity in PPLO broth and 15 lung and mediastinal lymph node samples had 'fried-egg colony' growth on the PPLO agar, which were suggestive of Mycoplasma infection. However, all were negative for MmmSC by PCR and immunoperoxidase. Thus, the northeastern part of Peninsular Malaysia remains positive serology for CBPP without isolation. |
| first_indexed | 2024-03-06T08:14:45Z |
| format | Article |
| id | upm.eprints-29520 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:14:45Z |
| publishDate | 2016 |
| publisher | Universiti Putra Malaysia Press |
| record_format | dspace |
| spelling | upm.eprints-295202016-08-02T03:01:01Z http://psasir.upm.edu.my/id/eprint/29520/ Seroprevalence and detection of contagious bovine pleuropneumonia (CBPP) in northeast states of Peninsular Malaysia M., Zarina Saad, Mohd Zamri Hassan, Latiffah Salisi, Mohd Shahrom O., Norlida Contagious Bovine Pleuropneumonia (CBPP) is a highly infectious disease of cattle caused by Mycoplasma mycoides subsp. mycoides Small Colony (MmmSC). It is a disease recognised by OIE that needs to be controlled or eradicated through surveillance system. This study establishes the sero-prevalence of CBPP, as well as attempts to isolate and identify the agent of CBPP from cattle in Kelantan and Terengganu, the northeastern states of Peninsular Malaysia, where cattle movement was high. A total of 3,242 sera from 428 farms were processed between 2011 and 2014 using the competitive ELISA (c-ELISA). The animal-level prevalence ranged between 5% (46/917) in Terengganu and 9% (220/2325) in Kelantan, whereas the herd prevalence ranged between 12% (22/210) in Terengganu and 24% (53/218) in Kelantan. The overall animal-level prevalence was 8% (266/3242), while the herd prevalence was 17% (75/428). Two hundred and four nasal swabs from 18 positive herds in Kelantan and 163 lung and mediastinal lymph node samples were processed for isolation of MmmSc before confirmation by PCR and immunoperoxidase. Forty-one samples showed turbidity in PPLO broth and 15 lung and mediastinal lymph node samples had 'fried-egg colony' growth on the PPLO agar, which were suggestive of Mycoplasma infection. However, all were negative for MmmSC by PCR and immunoperoxidase. Thus, the northeastern part of Peninsular Malaysia remains positive serology for CBPP without isolation. Universiti Putra Malaysia Press 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29520/1/10%20JTAS-0773-2015.pdf M., Zarina and Saad, Mohd Zamri and Hassan, Latiffah and Salisi, Mohd Shahrom and O., Norlida (2016) Seroprevalence and detection of contagious bovine pleuropneumonia (CBPP) in northeast states of Peninsular Malaysia. Pertanika Journal of Tropical Agricultural Science, 39 (2). pp. 257-265. ISSN 1511-3701; ESSN: 2231-8542 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2039%20(2)%20May.%202016/10%20JTAS-0773-2015.pdf |
| spellingShingle | M., Zarina Saad, Mohd Zamri Hassan, Latiffah Salisi, Mohd Shahrom O., Norlida Seroprevalence and detection of contagious bovine pleuropneumonia (CBPP) in northeast states of Peninsular Malaysia |
| title | Seroprevalence and detection of contagious bovine pleuropneumonia (CBPP) in northeast states of Peninsular Malaysia |
| title_full | Seroprevalence and detection of contagious bovine pleuropneumonia (CBPP) in northeast states of Peninsular Malaysia |
| title_fullStr | Seroprevalence and detection of contagious bovine pleuropneumonia (CBPP) in northeast states of Peninsular Malaysia |
| title_full_unstemmed | Seroprevalence and detection of contagious bovine pleuropneumonia (CBPP) in northeast states of Peninsular Malaysia |
| title_short | Seroprevalence and detection of contagious bovine pleuropneumonia (CBPP) in northeast states of Peninsular Malaysia |
| title_sort | seroprevalence and detection of contagious bovine pleuropneumonia cbpp in northeast states of peninsular malaysia |
| url | http://psasir.upm.edu.my/id/eprint/29520/1/10%20JTAS-0773-2015.pdf |
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