Chemopreventive effect of Ardisia crispa hexane fraction on the peri-initiation phase of mouse skin tumorigenesis
Objective: To investigate the chemopreventive effect of the hexane extract of Ardisia crispa during the peri-initiation phase of mouse skin tumorigenesis. Materials and Methods: This study was conducted for 12 weeks on two-stage 7,12-dimethylbenz(α)-anthracene (DMBA)-induced tumor initiation followe...
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| Format: | Article |
| Language: | English |
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Karger
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/29531/1/Chemopreventive%20effect%20of%20Ardisia%20crispa%20hexane%20fraction%20on%20the%20peri-initiation%20phase%20of%20mouse%20skin%20tumorigenesis.pdf |
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| author | Abd Hamid @ Abdul Razak, Roslida Othman, Fezah Anthony, Jacyln Janeris Yeong, Looi Ting |
| author_facet | Abd Hamid @ Abdul Razak, Roslida Othman, Fezah Anthony, Jacyln Janeris Yeong, Looi Ting |
| author_sort | Abd Hamid @ Abdul Razak, Roslida |
| collection | UPM |
| description | Objective: To investigate the chemopreventive effect of the hexane extract of Ardisia crispa during the peri-initiation phase of mouse skin tumorigenesis. Materials and Methods: This study was conducted for 12 weeks on two-stage 7,12-dimethylbenz(α)-anthracene (DMBA)-induced tumor initiation followed by croton-oil-induced tumor promotion in mice. A. crispa root hexane extract (ACRH) was applied at various doses (30, 100, 300 mg/kg) 7 days prior to and after DMBA treatment. Throughout the study, morphological observations, i.e., tumor incidence, tumor volume and tumor burden were measured for each of the treated groups. At the end of the experiment, the mice were sacrificed and their skin tissues were examined histopathologically. Results: The highest dose of ACRH (300 mg/kg) significantly delayed tumor formation (week 9, p < 0.05) and exhibited the lowest tumor volume (0.71 ± 0.00 mm3, p < 0.05), tumor burden (2.00 ± 0.00, p < 0.05), and tumor incidence (16.67%, p < 0.05) compared to other doses of ACRH. A 100-mg/kg dose produced tumor latency at week 7, tumor volume of 2.44 ± 0.88 mm3 (p < 0.05), tumor burden of 1.60 ± 0.60 (p < 0.05), and tumor incidence of 50%; 30 mg/kg produced tumor latency at week 8, tumor volume of 2.04 ± 0.45 mm3 (p < 0.05), tumor burden of 2.17 ± 0.54, tumor incidence of 60% and carcinogen control (tumor latency at week 7; tumor volume, 3.56 mm3; tumor incidence of 66.67%). Conclusion: The highest dose of A. crispa hexane extract delayed tumor development, thus showing a chemopreventive effect on mouse skin tumorigenesis. |
| first_indexed | 2024-03-06T08:14:47Z |
| format | Article |
| id | upm.eprints-29531 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:14:47Z |
| publishDate | 2013 |
| publisher | Karger |
| record_format | dspace |
| spelling | upm.eprints-295312016-10-31T01:59:12Z http://psasir.upm.edu.my/id/eprint/29531/ Chemopreventive effect of Ardisia crispa hexane fraction on the peri-initiation phase of mouse skin tumorigenesis Abd Hamid @ Abdul Razak, Roslida Othman, Fezah Anthony, Jacyln Janeris Yeong, Looi Ting Objective: To investigate the chemopreventive effect of the hexane extract of Ardisia crispa during the peri-initiation phase of mouse skin tumorigenesis. Materials and Methods: This study was conducted for 12 weeks on two-stage 7,12-dimethylbenz(α)-anthracene (DMBA)-induced tumor initiation followed by croton-oil-induced tumor promotion in mice. A. crispa root hexane extract (ACRH) was applied at various doses (30, 100, 300 mg/kg) 7 days prior to and after DMBA treatment. Throughout the study, morphological observations, i.e., tumor incidence, tumor volume and tumor burden were measured for each of the treated groups. At the end of the experiment, the mice were sacrificed and their skin tissues were examined histopathologically. Results: The highest dose of ACRH (300 mg/kg) significantly delayed tumor formation (week 9, p < 0.05) and exhibited the lowest tumor volume (0.71 ± 0.00 mm3, p < 0.05), tumor burden (2.00 ± 0.00, p < 0.05), and tumor incidence (16.67%, p < 0.05) compared to other doses of ACRH. A 100-mg/kg dose produced tumor latency at week 7, tumor volume of 2.44 ± 0.88 mm3 (p < 0.05), tumor burden of 1.60 ± 0.60 (p < 0.05), and tumor incidence of 50%; 30 mg/kg produced tumor latency at week 8, tumor volume of 2.04 ± 0.45 mm3 (p < 0.05), tumor burden of 2.17 ± 0.54, tumor incidence of 60% and carcinogen control (tumor latency at week 7; tumor volume, 3.56 mm3; tumor incidence of 66.67%). Conclusion: The highest dose of A. crispa hexane extract delayed tumor development, thus showing a chemopreventive effect on mouse skin tumorigenesis. Karger 2013-06 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29531/1/Chemopreventive%20effect%20of%20Ardisia%20crispa%20hexane%20fraction%20on%20the%20peri-initiation%20phase%20of%20mouse%20skin%20tumorigenesis.pdf Abd Hamid @ Abdul Razak, Roslida and Othman, Fezah and Anthony, Jacyln Janeris and Yeong, Looi Ting (2013) Chemopreventive effect of Ardisia crispa hexane fraction on the peri-initiation phase of mouse skin tumorigenesis. Medical Principles and Practice, 22 (4). pp. 357-361. ISSN 1011-7571; ESSN: 1423-0151 http://www.karger.com/Article/Abstract/346622 10.1159/000346622 |
| spellingShingle | Abd Hamid @ Abdul Razak, Roslida Othman, Fezah Anthony, Jacyln Janeris Yeong, Looi Ting Chemopreventive effect of Ardisia crispa hexane fraction on the peri-initiation phase of mouse skin tumorigenesis |
| title | Chemopreventive effect of Ardisia crispa hexane fraction on the peri-initiation phase of mouse skin tumorigenesis |
| title_full | Chemopreventive effect of Ardisia crispa hexane fraction on the peri-initiation phase of mouse skin tumorigenesis |
| title_fullStr | Chemopreventive effect of Ardisia crispa hexane fraction on the peri-initiation phase of mouse skin tumorigenesis |
| title_full_unstemmed | Chemopreventive effect of Ardisia crispa hexane fraction on the peri-initiation phase of mouse skin tumorigenesis |
| title_short | Chemopreventive effect of Ardisia crispa hexane fraction on the peri-initiation phase of mouse skin tumorigenesis |
| title_sort | chemopreventive effect of ardisia crispa hexane fraction on the peri initiation phase of mouse skin tumorigenesis |
| url | http://psasir.upm.edu.my/id/eprint/29531/1/Chemopreventive%20effect%20of%20Ardisia%20crispa%20hexane%20fraction%20on%20the%20peri-initiation%20phase%20of%20mouse%20skin%20tumorigenesis.pdf |
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