The combination of mitragynine and morphine prevents the development of morphine tolerance in mice
Mitragynine (MG) is the major active alkaloid found in Mitragyna speciosa Korth. In the present study, we investigated the enhancement of analgesic action of MG when combined with morphine and the effect of the combination on the development of tolerance towards morphine. Mice were administered intr...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI
2013
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/29542/1/29542.pdf |
| _version_ | 1796971560314077184 |
|---|---|
| author | Fakurazi, Sharida Abdul Rahman, Shamima Baharuldin, Mohamad Taufik Hidayat Ithnin, Hairuszah Mohd Moklas, Mohamad Aris Arulselvan, Palanisamy |
| author_facet | Fakurazi, Sharida Abdul Rahman, Shamima Baharuldin, Mohamad Taufik Hidayat Ithnin, Hairuszah Mohd Moklas, Mohamad Aris Arulselvan, Palanisamy |
| author_sort | Fakurazi, Sharida |
| collection | UPM |
| description | Mitragynine (MG) is the major active alkaloid found in Mitragyna speciosa Korth. In the present study, we investigated the enhancement of analgesic action of MG when combined with morphine and the effect of the combination on the development of tolerance towards morphine. Mice were administered intraperitoneally with a dose of MG (15 and 25 mg/kg b.wt) combined with morphine (5 mg/kg b.wt) respectively for 9 days. The antinociceptive effect was evaluated by a hot plate test. The protein expression of cyclic adenosine monophosphate (cAMP) and cAMP response element binding (CREB) was analyzed by immunoblot. Toxicological parameters especially liver and kidney function tests were assessed after the combination treatment with MG and morphine. The concurrent administration of MG and morphine showed significant (p < 0.05) increase in latency time when compared to morphine alone group and the outstanding analgesic effects in the combination regimens were maintained until day 9. For the protein expression, there was a significant increment of cAMP and CREB levels (p < 0.05) in group treated with 5 mg/kg morphine but there was no significant change of these protein expressions when MG was combined with morphine. There was a significant changes in toxicological parameters of various treated groups. The combination treatment of MG and morphine effectively reduce the tolerance due to the chronic administration of morphine. |
| first_indexed | 2024-03-06T08:14:49Z |
| format | Article |
| id | upm.eprints-29542 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:14:49Z |
| publishDate | 2013 |
| publisher | MDPI |
| record_format | dspace |
| spelling | upm.eprints-295422016-07-12T09:23:48Z http://psasir.upm.edu.my/id/eprint/29542/ The combination of mitragynine and morphine prevents the development of morphine tolerance in mice Fakurazi, Sharida Abdul Rahman, Shamima Baharuldin, Mohamad Taufik Hidayat Ithnin, Hairuszah Mohd Moklas, Mohamad Aris Arulselvan, Palanisamy Mitragynine (MG) is the major active alkaloid found in Mitragyna speciosa Korth. In the present study, we investigated the enhancement of analgesic action of MG when combined with morphine and the effect of the combination on the development of tolerance towards morphine. Mice were administered intraperitoneally with a dose of MG (15 and 25 mg/kg b.wt) combined with morphine (5 mg/kg b.wt) respectively for 9 days. The antinociceptive effect was evaluated by a hot plate test. The protein expression of cyclic adenosine monophosphate (cAMP) and cAMP response element binding (CREB) was analyzed by immunoblot. Toxicological parameters especially liver and kidney function tests were assessed after the combination treatment with MG and morphine. The concurrent administration of MG and morphine showed significant (p < 0.05) increase in latency time when compared to morphine alone group and the outstanding analgesic effects in the combination regimens were maintained until day 9. For the protein expression, there was a significant increment of cAMP and CREB levels (p < 0.05) in group treated with 5 mg/kg morphine but there was no significant change of these protein expressions when MG was combined with morphine. There was a significant changes in toxicological parameters of various treated groups. The combination treatment of MG and morphine effectively reduce the tolerance due to the chronic administration of morphine. MDPI 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29542/1/29542.pdf Fakurazi, Sharida and Abdul Rahman, Shamima and Baharuldin, Mohamad Taufik Hidayat and Ithnin, Hairuszah and Mohd Moklas, Mohamad Aris and Arulselvan, Palanisamy (2013) The combination of mitragynine and morphine prevents the development of morphine tolerance in mice. Molecules, 18 (1). pp. 666-681. ISSN 1420-3049 http://www.mdpi.com/1420-3049/18/1/666 10.3390/molecules18010666 |
| spellingShingle | Fakurazi, Sharida Abdul Rahman, Shamima Baharuldin, Mohamad Taufik Hidayat Ithnin, Hairuszah Mohd Moklas, Mohamad Aris Arulselvan, Palanisamy The combination of mitragynine and morphine prevents the development of morphine tolerance in mice |
| title | The combination of mitragynine and morphine prevents the development of morphine tolerance in mice |
| title_full | The combination of mitragynine and morphine prevents the development of morphine tolerance in mice |
| title_fullStr | The combination of mitragynine and morphine prevents the development of morphine tolerance in mice |
| title_full_unstemmed | The combination of mitragynine and morphine prevents the development of morphine tolerance in mice |
| title_short | The combination of mitragynine and morphine prevents the development of morphine tolerance in mice |
| title_sort | combination of mitragynine and morphine prevents the development of morphine tolerance in mice |
| url | http://psasir.upm.edu.my/id/eprint/29542/1/29542.pdf |
| work_keys_str_mv | AT fakurazisharida thecombinationofmitragynineandmorphinepreventsthedevelopmentofmorphinetoleranceinmice AT abdulrahmanshamima thecombinationofmitragynineandmorphinepreventsthedevelopmentofmorphinetoleranceinmice AT baharuldinmohamadtaufikhidayat thecombinationofmitragynineandmorphinepreventsthedevelopmentofmorphinetoleranceinmice AT ithninhairuszah thecombinationofmitragynineandmorphinepreventsthedevelopmentofmorphinetoleranceinmice AT mohdmoklasmohamadaris thecombinationofmitragynineandmorphinepreventsthedevelopmentofmorphinetoleranceinmice AT arulselvanpalanisamy thecombinationofmitragynineandmorphinepreventsthedevelopmentofmorphinetoleranceinmice AT fakurazisharida combinationofmitragynineandmorphinepreventsthedevelopmentofmorphinetoleranceinmice AT abdulrahmanshamima combinationofmitragynineandmorphinepreventsthedevelopmentofmorphinetoleranceinmice AT baharuldinmohamadtaufikhidayat combinationofmitragynineandmorphinepreventsthedevelopmentofmorphinetoleranceinmice AT ithninhairuszah combinationofmitragynineandmorphinepreventsthedevelopmentofmorphinetoleranceinmice AT mohdmoklasmohamadaris combinationofmitragynineandmorphinepreventsthedevelopmentofmorphinetoleranceinmice AT arulselvanpalanisamy combinationofmitragynineandmorphinepreventsthedevelopmentofmorphinetoleranceinmice |